<![CDATA[The purpose of this research was to evaluate antibacterial activity of Neem (Azadirachta indica A. Juss) leaves fraction against some pathogenic bacterial and to identificate the most active fraction. Leaves powder of Neem was macerated with ethanol and factionated by vacuum liquid chromatography using hexane, ethyl acetate and ethanol as eluent, respectively. The antibacterial activity of the fraction was evaluated by diffusion method, then the most active fraction of antibacterial was evaluated by zone of inhibition. The most active fraction of antibacterial was evaluated for Minimum Inhibitory Concentration (MIC) and equivalent value, compared with amoxicillin and chloramphenicol, then identified using phytochemical screening and Gass Chromatography-Mass Spectroscopy (GC-MS).The fraction of Neem leaves fractionated by vacuum liquid chromatography had antibacterial activity againstStaphylococcus epidermidis, Bacillus cereus, and Shigella flexneri. The ethyl acetate fraction showed the most active fraction of antibacterial against all bacterial tested. Ethyl acetate fraction had MIC 0.075% againstStaphylococcus epidermidis 0.05% against Bacillus cereus and Shigella flexneri. The antibacterial activity of ethyl acetat fraction compared to amoxicillin was 0.01% for S. epidermidis, 0.02% for B. cereus, and 0.02% for S. flexneri. Then compared to chloramphenicol was 0.04% for S. epidermidis, 0.02% for B. cereus, and 0.06% for S. flexneri. The result of GC-MS showed that the ethyl acetate fraction contained palmitic acid, ethyl linoleolate, stearic acid, trans-phytol, DOP (di octyl phthalate), and suggested 1 class of compound fatty acid and 5 classes of compound triterpenoids.]]>
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