RP-HPLC Method Development and Validation for the Estimation of Methylcobalamin in Bulk

BS Muddukrishna

Journal of Global Pharma Technology
Volume 09 Issue 03

The subsequent artefact entail of extremely subtle and informal Reversed phase HPLC (RP-HPLC) technique for approximation ofMethylcobalamin in large quantity. Shimadzu LC-20-AD(Kyoto, Japan), with auto sampler and (PDA/UV) detector was used for the separation of Methylcobalamin.C18 Hypersil BDS (5.0 micron,150 x 4.6mm) column was aimed at the separation and mobile phase(MP) comprising of(55:45 v/v) Methanol:o-phosphoric acid 0.02%v/v pH 2.3 was cast-offby keeping the rate offlow1 ml/minalong the effluents supervised on 223nm. linearity is undeviating having line equationf(x) =4.87512e-006*x-0.702284 with correlation coefficient(R2) of 0.997. Theprocessremained precise with 0.5% RSD(intra-day precision)&(Inter-day precision) is 0.43%. The LOD & LOQ was0.1499μg/ml and 0.4542μg/ml respectively.The establishedprocess wasvalidated forlinearity,system suitability,precision, and robustness; henceforthverifies in order that this one is exceedinglyrelevantto approximate Methylcobalamin in routine analysis.Methods name: Estimation of Methylcobalaminby HPLC.Keywords: Methylcobalamin, Reverse phase hplc (RP-HPLC), Validation and chromatography.