Human sperm DNA integrity : A difference of storage temperature and period of freeze-drying process

silvia werdhy lestari (Universitas Indonesia)
ahmad silalahi (Andrology Specialist Program, Medical Faculty of the University of Airlangga / Dr. Soetomo Hospital Surabaya, Indonesia)
hamdani lunardi (Medical Biology Department, Medical Faculty of the University of Airlangga, Surabaya, Indonesia.)
agustinus agustinus (Medical Biology Department, Medical Faculty of the University of Airlangga, Surabaya, Indonesia.)

Journal of Global Pharma Technology
Volume 12 Issue 09 (2020) Sept. 2020

The cryopreserve technique using liquid nitrogen is used to preserve human sperm. Nevertheless, this technique has several disadvantages, such as bacterial/viral contamination and its expensive cost. Freeze-drying process offers more simple and cheaper of sperm storage technique. This study aimed to investigate the effect of storage temperature and period of freeze-dried human sperm on DNA integrity. Human sperm samples of 15 donors were prepared with simple washing techniques. Post-washing samples (T0) were divided into 4 groups with the same volume. The four group of samples carried on the freeze-drying process and stored based on different temperature and period: 4°C for 1 week (T1), room temperature (24-25ºC) for 1 week (T2), 4°C for 3 months (T3), and room temperature for 3 months (T4). Sperm DNA integrity was analyzed before and after the storage period of each group. There was a statistically significant difference of sperm DNA integrity of freezes - dried at 4°C in 1 week versus 3 months (T1 vs T3), at room temperature in 1 week versus 3 months (T2 vs T4) (p