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silvia werdhy lestari
Universitas Indonesia
Medicine & Pharmacology

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Human sperm DNA integrity : A difference of storage temperature and period of freeze-drying process silvia werdhy lestari; ahmad silalahi; hamdani lunardi; agustinus agustinus
Journal of Global Pharma Technology Volume 12 Issue 09 (2020) Sept. 2020
Publisher : Journal of Global Pharma Technology

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The cryopreserve technique using liquid nitrogen is used to preserve human sperm. Nevertheless, this technique has several disadvantages, such as bacterial/viral contamination and its expensive cost. Freeze-drying process offers more simple and cheaper of sperm storage technique. This study aimed to investigate the effect of storage temperature and period of freeze-dried human sperm on DNA integrity. Human sperm samples of 15 donors were prepared with simple washing techniques. Post-washing samples (T0) were divided into 4 groups with the same volume. The four group of samples carried on the freeze-drying process and stored based on different temperature and period: 4°C for 1 week (T1), room temperature (24-25ºC) for 1 week (T2), 4°C for 3 months (T3), and room temperature for 3 months (T4). Sperm DNA integrity was analyzed before and after the storage period of each group. There was a statistically significant difference of sperm DNA integrity of freezes - dried at 4°C in 1 week versus 3 months (T1 vs T3), at room temperature in 1 week versus 3 months (T2 vs T4) (p
Immunohistochemistry Detection Method of Rejection Reaction of Human Umbilical Cord Derived Mesenchymal Stem Cell on Rat Sciatic Nerve Tissue Ria Margiana; Ahmad Aulia Jusuf; Silvia Werdhy Lestari
Journal of Global Pharma Technology Volume 10 Issue 07: (2018) July 2018
Publisher : Journal of Global Pharma Technology

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Abstract:Human umbilical cord derived mesenchymal stromal cells (UC-MSCs) that are accessible from cell banks can be actuated to separate into different cell sorts, accordingly making them handy potential hotspots for cell-based treatments. In injured peripheral nerves, Schwann cells (SCs) add to useful recuperation by supporting axonal recovery and myelin remaking. Here, we first exhibit a framework to induce UC-MSCs to separate into cells with SC properties (UC-SCs) by treatment with β-mercaptoethanol took after by retinoic corrosive and an arrangement of particular cytokines. The UC-SCs are morphologically like SCs and express SC markers, including P0, as evaluated by immunocytochemistry and converse interpretation polymerase chain response. Transplantation of UC-SCs into transected sciatic nerves in grown-up rats improved nerve recovery. The viability of UC-SCs for axonal recovery was tantamount to that of legitimate human SCs taking into account histological criteria and useful recuperation. Immunohistochemistry and immunoelectron microscopy additionally showed myelination of recovered axons by UC-SCs. These discoveries demonstrate that cells with SC properties and with the capacity to bolster axonal recovery and reproduce myelin can be effectively actuated from UC-MSCs to advance practical recuperation after peripheral nerve injury. This framework might be pertinent for the advancement of cell-based treatments.
Sperm DNA Fragmentation and Chromatin Maturation of Prepared Sperm with ALA Supplementation: The Effect of Embryo Development Quality silvia werdhy lestari; aucky hinting; Petrus Supardi; anom Bowolaksono; asmarinah asmarinah
Journal of Global Pharma Technology Volume 11 Issue 09: (2019) September 2019
Publisher : Journal of Global Pharma Technology

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Abstract: This study was aimed to determine the differences in embryo quality between spermatozoa with good or poor of DNA fragmentation index and chromatin maturation, through spermatozoa preparation with the addition of ALA. This experimental study compared the quality of embryos that fertilized by spermatozoa with DNA fragmentation and chromatin maturation, with and without ALA supplementation. In this study semen samples from 20 patients were collected, which were then prepared and examined for DNA fragmentation index (DF) and chromatin maturation (CM). Then the sample was divided into 4 groups, namely the good DF - good CM, good DF - poor CM, poor DF - good CM and poor CM - poor CM, further divided again with and without treatment (ALA supplementation). After injection of spermatozoa into the oocyte, the development of embryo was observed in each group. In the group without treatment, significant differences in embryo quality were obtained between groups of good DF - good CM with poor DF - good CM, good DF - good CM with poor DF - poor CM, good DF - poor CM with poor DF - good CM and good DF - poor CM with poor DF - poor CM (p <0.05). In the treatment group, there were not significant difference in the quality of the embryo between treatment groups, namely with or without ALA, in the group with good or poor DF, and with good or poor CM (p> 0.05). Embryo fertilized by spermatozoa with good or poor DF and with good or poor CM, with the supplementation of ALA, the quality was not significantly better than without the supplementation of ALA.    Keywords: spermatozoa DNA fragmentation, spermatozoa chromatin maturation, ALA, in vitro fertilization, embryo quality 
Co-Authors agustinus agustinus Ahmad Aulia Jusuf ahmad silalahi ANOM BOWOLAKSONO Asmarinah Asmarinah Aucky Hinting hamdani lunardi Petrus Supardi Ria Margiana