Medical Technology and Public Health Journal
Vol 7 No 1 (2023): Medical Technology and Public Health Journal March 2023

Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD

setiyo rini, chylen (Unknown)



Article Info

Publish Date
26 Apr 2023

Abstract

Kaffir lime (Citrus hystrix) is a horticultural plant that is widely used as a natural flavor in various food and beverage products. The content in lime leaves is useful for health, including flavonoids, minerals, vitamin C, limonoids, and carotenoids. Bacillus cereus causes a diarrheal disease that attacks the intestines. This study aims to determine the kaffir lime leaf extract in inhibiting the growth of Bacillus cereus bacteria using the Time-Kill Test method. This study used the Minimum Inhibitory Concentration (MIC) and Time-Kill Test methods. The MIC results in kaffir lime leaf extract that can inhibit bacteria at 2 mg/mL, while the Time-Kill Test shows a death phase at 24 hours. Kaffir lime leaf extract has a bacteriostatic effect at 0, 4, 8, and 24 hours, while amoxicillin and the combination of amoxicillin and extract have a bacteriostatic effect at 4, 8, and 24 hours. Based on the results of the One Way ANOVA test with a 95% confidence level (p <0,05) showed a significant effect of kaffir lime leaf extract on the growth of Bacillus cereus.

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Journal Info

Abbrev

MTPHJ

Publisher

Subject

Environmental Science Health Professions Nursing Public Health

Description

Medical Technology and Public Health Journal with registered number ISSN 2549-189X (printed) and e-ISSN 2549-2993 (online) is a scientific open access journal published by Health Faculty, Universitas Nahdlatul Ulama Surabaya, Indonesia. Our journal aims to become a media of research results ...