Analysis of Urine Podocalyxin in Type 2 Diabetes Mellitus Subjects With and Without Diabetic Nephropathy

Jusni Ekasari Pelu (Master of Biomedical Sciences, Graduate School Hasanuddin University, Makassar, South Sulawesi, Indonesia)
Liong Boy Kurniawan (Master of Biomedical Sciences, Graduate School Hasanuddin University, Makassar, South Sulawesi, Indonesia)
Yuyun Widaningsih (Hasanuddin University Hospital, Makassar, South Sulawesi, Indonesia)
Alfian Zainuddin (Department of Public Health and Community Medicine, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia)
Husaini Umar (Department of Internal Medicine, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia)
Nurahmi Nurahmi (Department of Clinical Pathology, Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia)
Theosobia Grace Orno (Department of Medical Laboratory Technology, Health Polytecnhnic of Health Ministry Kendari, Southeast Sulawesi, Indonesia)

INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care

Type 2 diabetes mellitus is the most common cause of diabetes, consist from about 85% of cases. Diabetic nephropathy is a complication of diabetes mellitus in the kidneys which can end up as kidney failure. Podocalyxin (PDX) is a protein expressed in kidney podocytes that is involved in various cancers, and is also essential for kidney development. The research design was carried out using observational and cross-sectional analytic methods with total participants of 25 DM with diabetic nephropathy and 25 DM without diabetic nephropathy with a purposive probability sampling technique. This research conducted at the Endocrine Polyclinic, Clinical Pathology Laboratory, Hasanuddin University Medical Research Center (HUM-RC) Laboratory, Hasanuddin University Hospital, Makassar. The results showed that the urinary PDX level in DM subjects with nephropathy were 1.160 ng/mL and DM without nephropathy were 0.167 ng/mL (p<0.001), the urine albumin/creatinine ratio (ACR) of DM subjects with nephropathy were 644.74 mg/ g and DM without nephropathy of 10.071 mg/g (p<0.001) and the correlation test results of urine PDX and urine ACR in DM subjects with nephropathy (r=0.510; p=0.001). This study concluded that there was a significant difference between urinary PDX in DM with and without diabetic nephropathy, there was a significant difference between urine ACR levels in DM with and without diabetic nephropathy, and there was a relationship between urinary PDX levels and urine ACR in DM subjects with diabetic nephropathy.