Articles
<![CDATA[Correlation Between Onset of Diabetes Mellitus and Nitric Oxide Levels in Patient with Type 2 Diabetes Mellitus ]]>
<![CDATA[Theosobia Grace Orno]]>;
<![CDATA[Mansyur Arif]]>;
<![CDATA[Irfan Idris]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 4 No. 1 (2018): June ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v4i1.175
<![CDATA[Type 2 diabetes mellitus (DM) is associated with increased risk of endothelial dysfunction if it lasts a long time without control. This study aims to connect the Onset of Diabetes Mellitus (DM) with Nitric Oxide levels in patients of type 2 diabetes mellitus. The study used cross-sectional study method. The samples were 86 subjects, consisting of 38 subjects of Type 2 DM controlled and 48 subjects of Type 2 DM uncontrolled. The results of the Kruskal-Wallis statistical test showed no significant difference between the Onset of DM and Nitric Oxide levels in the categories of 4-6 years (19.4 ± 10.1), 7-9 years (17.3 ± 9.3) and 10-12 years (13.3 ± 8.5) (p=0.06). Furthermore, the Spearman correlation test revealed a negative correlation between the Onset of DM and Nitric Oxide level in patients with Type 2 DM with and without control (r =-0.217). The level of Nitric Oxide (NO) can consider as a predictor of long-term complication in patients with type 2 DM.]]>
<![CDATA[INVITRO CITOTOXICITY ASSAYS OF SEAGRASS (Enhalus acoroides) METHANOL EXTRACT FROM SOROPIA COASTAL WATERS SOUTHEAST SULAWESI REGENCY ]]>
<![CDATA[Theosobia Grace Orno]]>;
<![CDATA[Agnes Rantesalu]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v2i1.1463
<![CDATA[The studies analysing the use of natural ingredients as an alternative treatment in the field of pharmacology are developing very rapidly. One of researches that is quite promising in the pharmaceutical industry is the application of marine materials. Marine materials that are frequently used consist of shellfish, algae, sponges and seagrass. Several studies on toxicity tests have shown that the methanol extract of seagrass (from species Enhalus acoroides) is more toxic than the other seagrass family. This study aims to test the toxicity level of Seagrass (E. acoroides) extract from Soropia coastal waters. The research method in this study was an experimental laboratory using E. acoroides Seagrass as a sample that was obtained from Soropia Coast, Konawe Regency, Southeast Sulawesi Province. The sample was extracted using methanol as a solvent by macerating it and was tested for its toxicity using the Brine Shrimp Lethality Test (BSLT) method. Toxicity test results showed that the samples with a concentration of 10 ppm, 100 ppm and 1000 ppm in leaves extracts produced an LC50 value of 404.88 ppm, while the stem and root extracts has a value of LC50 >1000 ppm. The test was continued with higher concentration of leaves extracts consisted of 250 ppm, 500 ppm, and 1000 ppm. The toxicity test showed an LC50 value of 0.7309; which means that it was very toxic. The methanol extract of Seagrass (E. Acoroides) is potential to be used for further analysis and anticancer formulations.]]>
<![CDATA[THE EARLY DETECTION OF METABOLIC SYNDROME USED NCEP ATP-III METHOD IN WAAI VILLAGE COMMUNITY CENTRAL MALUKU REGENCY ]]>
<![CDATA[Theosobia Grace Orno]]>
<![CDATA[ Molucca Medica Vol 15 No 1 (2022): VOLUME 15, NOMOR 1, APRIL 2022 ]]>
Publisher : <![CDATA[Pattimura University ]]>
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DOI: 10.30598/molmed.2022.v15.i1.1
<![CDATA[Metabolic syndrome is a metabolic disorder that complexly includes carbohydrate, lipid and protein metabolism disorders which are known to be the main cause of diabetes mellitus, hypertension and coronary heart disease. Waai Village, Central Maluku Regency is one of the villages affected by the earthquake for a long time so that social assistance in the form of fast food is consumed in the long term which is thought to be one of the causes of metabolic syndrome. The purpose of this research is to detect the presence of metabolic syndrome with the NCEP ATP-III criteria consists of measuring blood pressure, fasting blood glucose levels and serum triglyceride levels using the photometric method. This type of research is descriptive research with a cross sectional approach. The sampling technique used is purposive sampling, in accordance with the predetermined sample criteria. The results showed that 29 of 50 subjects (58%) had metabolic syndrome with an average blood pressure of 148/94 mmHg, an average fasting blood glucose level of 158 mg/dL, and an average triglyceride level of 198 mg/dL.]]>
<![CDATA[Correlation Analysis of Interleukin-6 on Blood Glucose in Prediabetes and Normal Glycemic Status ]]>
<![CDATA[Ratih Feraritra Danu Atmaja]]>;
<![CDATA[Theosobia Grace Orno]]>;
<![CDATA[Tuty Yuniarty]]>;
<![CDATA[Handayani Malik]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 9 No. 1 (2023): June ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v8i2.496
<![CDATA[Diabetes mellitus is a global health problem whose incidence rate continues to increase yearly. Most people with diabetes mellitus go through the prediabetes phase. Prediabetes is a condition where blood glucose levels are elevated but have not yet reached the criteria for diabetes mellitus. Low-grade chronic inflammation is one of the pathways known to interfere with insulin signalling that ultimately affects blood glucose levels. One of the most studied inflammatory pathways in the pathogenesis of diabetes mellitus is interleukin-6 (IL-6). This study aims to determine whether there were differences in IL-6 levels between groups of prediabetes subjects and normal subjects and to observe the correlation between IL-6 levels and blood glucose. This study is useful in providing additional scientific evidence on the development of diabetes mellitus, especially in blood glucose regulation through inflammatory pathways. The design of this study was analytic observational in 71 subjects with prediabetes or normal glycemic status. Prediabetes status was established based on fasting blood glucose levels and glucose levels 2 hours post oral glucose tolerance test. Subjects with fasting blood glucose levels> 125mg/ dl and who had a fever in the last week were excluded from the study. Interleukin 6 levels were measured based on the principle of enzyme-linked immunoassay. The correlation of interleukin 6 with glucose levels and other variables was analyzed using the spearmen test. The results showed that interleukin 6 levels did not differ between the prediabetes group and the normal group ((5.27 ± 2.55 pg/ml) vs (4.44 ± 2.46) respectively; (p=0.105)). There was no correlation between interleukin 6 level and fasting blood glucose level (r=0.014, p=0.908) and glucose level after the oral glucose tolerance test (r=-0.085, p=0.480). In this study, there was a significant correlation between body mass index with waist circumference (r=0.772, p=0.000) and glucose levels after the oral glucose tolerance test (r=0.240; p<0.001). Recommends the addition of anti-inflammatory cytokines and variable insulin to assess further the effect of the inflammatory process on the glucose metabolism of subjects in future studies.]]>
<![CDATA[Analysis of Urine Podocalyxin in Type 2 Diabetes Mellitus Subjects With and Without Diabetic Nephropathy ]]>
<![CDATA[Jusni Ekasari Pelu]]>;
<![CDATA[Liong Boy Kurniawan]]>;
<![CDATA[Yuyun Widaningsih]]>;
<![CDATA[Alfian Zainuddin]]>;
<![CDATA[Husaini Umar]]>;
<![CDATA[Nurahmi Nurahmi]]>;
<![CDATA[Theosobia Grace Orno]]>
<![CDATA[ JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care ]]>
Publisher : <![CDATA[Universitas Nahdlatul Ulama Surabaya ]]>
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DOI: 10.33086/ijmlst.v5i1.3933
<![CDATA[Type 2 diabetes mellitus is the most common cause of diabetes, consist from about 85% of cases. Diabetic nephropathy is a complication of diabetes mellitus in the kidneys which can end up as kidney failure. Podocalyxin (PDX) is a protein expressed in kidney podocytes that is involved in various cancers, and is also essential for kidney development. The research design was carried out using observational and cross-sectional analytic methods with total participants of 25 DM with diabetic nephropathy and 25 DM without diabetic nephropathy with a purposive probability sampling technique. This research conducted at the Endocrine Polyclinic, Clinical Pathology Laboratory, Hasanuddin University Medical Research Center (HUM-RC) Laboratory, Hasanuddin University Hospital, Makassar. The results showed that the urinary PDX level in DM subjects with nephropathy were 1.160 ng/mL and DM without nephropathy were 0.167 ng/mL (p<0.001), the urine albumin/creatinine ratio (ACR) of DM subjects with nephropathy were 644.74 mg/ g and DM without nephropathy of 10.071 mg/g (p<0.001) and the correlation test results of urine PDX and urine ACR in DM subjects with nephropathy (r=0.510; p=0.001). This study concluded that there was a significant difference between urinary PDX in DM with and without diabetic nephropathy, there was a significant difference between urine ACR levels in DM with and without diabetic nephropathy, and there was a relationship between urinary PDX levels and urine ACR in DM subjects with diabetic nephropathy.]]>
<![CDATA[SKRINING ANEMIA MELALUI PEMERIKSAAN LABORATORIUM PADA MASYARAKAT PESISIR DESA MEKAR KECAMATAN SOROPIA ]]>
<![CDATA[Julianti Isma Sari]]>;
<![CDATA[Theosobia Grace Orno]]>;
<![CDATA[Fonnie Esther Hasan]]>
<![CDATA[ PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 3 No 1 (2023): Pakem : Jurnal Pengabdian Kepada Masyarakat ]]>
Publisher : <![CDATA[Universitas Pattimura ]]>
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DOI: 10.30598/pakem.3.1.7-12
<![CDATA[Anemia was detected from laboratory tests with hemoglobin, hematocrit and erythrocyte levels below the reference value. Anemia can be detected with Point of Care Testing method using and is effective for conducting examinations in areas with a relatively small number of health facilities. The type of method used is analytic observation. This community service aims to screen for anemia by knowing hemoglobin and hematocrit levels. The results of the examination showed that from 50 respondents, the average hemoglobin level had a result below the reference value of 12.0 – 17.0 g/dl and the hematocrit value having a result below the reference value of 37-48%. The average hemoglobin value for men is 12.0 g/dl and for women is 11.9 g/dl. The average hematocrit value for both men and women is 35%. %. With anemia screening conducted, the public can find out hemoglobin and hematocrit levels as an effort to prevent anemia]]>
<![CDATA[Eliminasi Vektor Demam Berdarah Dengue melalui Penyuluhan dan Pemasangan Ovitrap di Kelurahan Puuwatu Kecamatan Puuwatu ]]>
<![CDATA[Reni Yunus]]>;
<![CDATA[Supiati Supiati]]>;
<![CDATA[Theosobia Grace Orno]]>
<![CDATA[ Jurnal Inovasi, Pemberdayaan dan Pengabdian Masyarakat Vol 1 No 1 (2021): Mei ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Kendari ]]>
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DOI: 10.36990/jippm.v1i1.244
<![CDATA[Dengue Hemorrhagic Fever (DHF) is a contagious disease that occurs due to infection with the Dengue virus, which is carried by the Aedes aegypti mosquito as the main vector. Research on ovitrap placement in DHF endemic areas has been conducted before in Kendari City. The results of this research need to be implemented in the form of community service activities. This community service activity aims to increase stakeholder understanding through dissemination of research results in the Puuwatu village area, increase public knowledge about the concept of handling dengue fever and its prevention, and to install ovitraps to find out how much success ovitrap has in reducing adult mosquito density. Post-test results after counseling about DHF showed an increase in public knowledge about DHF and its prevention. Ovitrap observations posted on 50 houses of Puuwatu village residents showed that ovitrap installed in all zones contained positive ovitrap eggs of Aedes sp.]]>
<![CDATA[Basic Medical Laboratory Services as an Effort to Detect Metabolic Syndrome in Anduonohu Sub-District, Kendari City ]]>
<![CDATA[Ratih Feraritra Danu Atmaja]]>;
<![CDATA[Theosobia Grace]]>;
<![CDATA[Tuty Yuniarty]]>;
<![CDATA[Julianti Isma Sari]]>
<![CDATA[ Jurnal Berkala Kesehatan Vol 9, No 1 (2023): JURNAL BERKALA KESEHATAN ]]>
Publisher : <![CDATA[Lambung Mangkurat ]]>
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DOI: 10.20527/jbk.v9i1.14530
<![CDATA[Metabolic syndrome is an accumulation of metabolic disorders that can increase the risk of cardiovascular-related diseases and type 2 diabetes mellitus. Metabolic syndrome is characterized by central obesity, hyperglycemia, hypertriglyceridemia, hypertension and decreased of HDL level. Community basically already knows each parameter separately but still rarely knows about the condition if these parameters occur together as metabolic syndrome. This study aimed to socialize as well as brought laboratory services closer to detect metabolic syndrome. This activity was carried out on 50 residents of the Anduonohu Village, Poasia District, Kendari City. Laboratory services performed include measurement of body mass index (BMI), waist circumference, blood pressure, blood glucose and blood cholesterol. Detection of metabolic syndrome using the definition of the International Diabetes Foundation 2009 by assessing the parameters of central obesity, blood pressure and glucose levels showed that 48% of the participants had metabolic syndrome]]>
<![CDATA[EFEKTIVITAS EKSTRAK ETANOL DAUN KERSEN (Muntingia Calabura) TERHADAP PROFIL HISTOLOGI HEPAR TIKUS DIABETES ]]>
<![CDATA[Theosobia Grace Orno]]>;
<![CDATA[Aswiro Hasan]]>;
<![CDATA[Fonnie Esther Hasan]]>
<![CDATA[ Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023 ]]>
Publisher : <![CDATA[Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar ]]>
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DOI: 10.33992/meditory.v11i1.2403
<![CDATA[Background: Diabetes mellitus occurs due to insulin resistance or absolute insulin deficiency as a result of damage to pancreatic beta cells. Long-term conditions of gluconeogenesis lead to auto-oxidation of glucose, protein glycation and activation of the polyol pathway which result in increased production of free radicals which is characterized by the formation of reactive oxygen compounds. Antioxidant compounds contained in Muntingia calabura L. leaves are expected to be able to protect liver cells from damage caused by free radicals with flavonoids as the dominant active compound. Aims: This study aims to test the effectiveness of Muntingia calabura L. leaf ethanol extract on the histological profile of diabetic rats.Methode:This research method is a laboratory experimental research, tissue staining using the hematoxylin eosin staining method. The result : showed that the liver histology of diabetic ratsafter therapy with ethanol extract of Muntingia calabura L. leaves at dose I, dose II and dose III did not appear to be significantly different. Similar to the group of DM rats treated with glibenclamide, the hepatocyte cells appeared to be still expe riencing enlargement, widening of the sinusoids and changes in parenchymatous cells. In addition, the ethanol extract of Muntingia calabura L. leaves dose I, II and III were also unable to regenerate the central vein. Conclusion: ethanol extract of Muntingia calabura L. leaf dose groups I, II and III were not effective in repairing hepatocyte necrosis.]]>
<![CDATA[Analysıs of Urınary Albumın and Urınary Synaptopodın Levels in Type 2 Dıabetes Mellıtus Subjects ]]>
<![CDATA[Afni Juhairia Laisouw]]>;
<![CDATA[Liong Boy Kurniawan]]>;
<![CDATA[Yuyun Widaningsih]]>;
<![CDATA[Tenri Esa]]>;
<![CDATA[Himawan Sanusi]]>;
<![CDATA[Andi Alfian Zainuddin]]>;
<![CDATA[Theosobia Grace Orno]]>
<![CDATA[ Medical Laboratory Technology Journal Vol. 9 No. 1 (2023): June ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan ]]>
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DOI: 10.31964/mltj.v8i2.514
<![CDATA[Hyperglycemia from diabetes mellitus affects many body organs and interferes with normal function. Kidney function decreased in approximately one-third of patients with diabetes mellitus before the development of albuminuria. The purpose of this study was to analyze differences in urine albumin and urine synaptopodin levels in type 2 diabetes mellitus (T2DM) and non-DM subjects, differences in urine synaptopodin levels in T2DM subjects with and without nephropathy, to analyze the correlation between urine albumin and urine synaptopodin in T2DM subjects and the cut-off analysis of sensitivity and specificity of urinary synaptopodin in diagnosing diabetic nephropathy. A sample of 60 subjects comprised 40 T2DM subjects and 20 non-DM subjects. Urinary synaptopodin levels were examined using the ELISA method, and albuminuria levels using the immunoturbidimetric method. Based on statistical analysis, the results showed that there were differences in urine albumin levels in T2DM and non-DM subjects (p*= < 0.001), there were differences in urine synaptopodin levels in T2DM and non-DM subjects (p*= < 0.001), there were no differences in urine synaptopodin levels with and without nephropathy in T2DM subjects (p*= 0.090), a relationship was found between urine albumin and urinary synaptopodin in T2DM subjects (p*= 0.048, r= 0.314) and the cut off of urinary synaptopodin in diagnosing nephropathy was ³0.39 ng/mL, sensitivity 64.7% and specificity 56.5%. We recommend further prospective studies with larger sample sizes to compare urinary synaptopodin levels and microalbuminuria (MAU) as markers for early detection of DN in T2DM subjects.]]>
