PAMERI : Pattimura Medical Review
Vol 3 No 1 (2021)

UJI AKTIVITAS ANTIOKSIDAN ALGA COKELAT Saragassum sp. DENGAN METODE 1,1- DIFENIL-2-PIKRIHIDRASIL (DPPH)

Anugrah P.M.D. Kamoda (Fakultas Kedokteran Universitas Pattimura)
Maria Nindatu (Fakultas Kedokteran Universitas Pattimura)
Indrawanti Kusadhiani (Fakultas Kedokteran Universitas Pattimura)
Eka Astuty (Fakultas Kedokteran Universitas Pattimura)
Halidah Rahawarin (Fakultas Kedokteran Universitas Pattimura)
Elpira Asmin (Fakultas Kedokteran Universitas Pattimura)



Article Info

Publish Date
22 Jun 2021

Abstract

Antioxidants are chemical compounds that are naturally present in the human body, which can donate hydrogen atoms to free radicals, resulting in chain reactions and converting free radicals into stable forms. Based on the source, antioxidants can be divided into 2 namely natural antioxidants and synthetic antioxidants that can overcome the formation of free radicals. One of the natural ingredients that can overcome the formation of free radicals is the brown algae Sargassum sp. The purpose of this study was to test antioxidant activity using the 1,1-Diphenyl-2-Pikrihydrasil (DPPH) method. This research is a type of experimental laboratory research. The research methodology included and prepared ingredients, extract preparation, and antioxidant activity testing using the DPPH method. Data analysis in this study was carried out by calculating the percent (%) of activity obtained from the absorbance of the data from each concentration. After obtaining the percent (%) antioxidant activity data for each absorbance sample, the IC50 value is calculated using a non-linear regression equation by substituting y = ax + b which states the relationship between log concentration and percent (%) antioxidant activity (inhibition). The results showed 50% concentration inhibition (IC50) of brown algae Sargassum sp. is 5,864. So it can be conclude that the algae Sargassum sp. has a very active antioxidant activity.

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Journal Info

Abbrev

pameri

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Description

PAMERI is a peer-reviewed and open access journal that focuses on promoting medical sciences generated from basic sciences, clinical, and community research to integrate researches in all aspects of human health. This journal publishes original articles, reviews, and also interesting case reports. ...