International Journal of Agricultural Sciences
Vol 6, No 2 (2022)

Nanoemulsion from Piper aduncum, Cymbopogon nardus, and Bacillus thuringiensis to Control Xanthomonas axonopodis pv. allii

Ly Lan Phuong (Student of Plant Protection, Post Graduated Program, Andalas University, Kampus Unand Limau Manis, Padang 25163, Indonesia)
Eka Candra Lina (Plant Protection Department, Faculty of Agriculture, Andalas University, Kampus Unand Limau Manis, Padang 25163, Indonesia)
Yulmira Yanti (Plant Protection Department, Faculty of Agriculture, Andalas University, Kampus Unand Limau Manis, Padang 25163, Indonesia)



Article Info

Publish Date
31 Dec 2022

Abstract

The bacterial species Xanthomonas axonopodis pv allii (Xaa) is an important pathogen causing leaf blight in shallots. The use of botanical pesticides with nanoemulsion formulations has become a common alternative. This study aims to determine the characteristics and optimum concentration of the mixture of essential oil of Piper aduncum and fragrant Cymbopogon nardus waste. Nanoemulsion formulations are made using spontaneous emulsification methods. Besides, testing Bacillus thuringiensis strain MRSNR3.1 and its secondary metabolites toxicity against Xaa was carried out by the diffusion method using paper discs to determine the diameter of the inhibition zone. The results demonstrate that all four concentrations, 1%, 2.5%, 5%, and 7.5%, could control Xaa bacteria. A concentration of 1% is considered more optimal than the other three   concentrations in bactericidal effects against Xaa, as manifested in the formed clear zone (diameter of 3.17 cm). Besides, Bacillus thuringiensis strain MRSNR3.1 and its secondary metabolites were also effective againstXaa after four days of incubation with inhibition zones of 3.04 ± 0.44and 2.21 ± 0.28, respectively. Hence, it is concluded that nanoemulsion at 1% concentration and Bacillus thuringiensis strain MRSNR3.1 have  bactericidal properties that can be used to control Xaa

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Journal Info

Abbrev

ijac

Publisher

Subject

Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Social Sciences

Description

International Journal of Agricultural Sciences is an open access journal published by the Graduate Program of Andalas University. IJASC publishes original research findings from throughout the world related to strategic and applied studies in all aspects of agricultural science, as well as reviews ...