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FORMULASI PADAT RHIZOBAKTERIA INDIGENUS BACILLUS THURINGIENSIS TS2 DAN WAKTU PENYIMPANAN UNTUK MENGENDALIKAN PENYAKIT PUSTUL BAKTERI XANTHOMONAS AXONOPODIS PV. GLYCINES
Yanti, Yulmira;
Habazar, Trimurti;
Resti, Zurai
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 1 (2017): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung
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DOI: 10.23960/j.hptt.1179-18
Solid formulations of indigenous rhizobacteria Bacillus thuringiensis TS2 and storage time to control bacterial pustule disease Xanthomonas axonopodis pv. glycines. Bacterial pustule disease caused by Xanthomonas axonopodis pv. Glycines is a major constraint in soybean cultivation. Indigenous rhizobacteria Bacillus thuringiensis TS2 from soybean rhizosphere acquired from previous research is the best isolate which can control soybean bacterial pustule disease and increase growth rate of soybean. To increased its stability and interaction with soybean plants, Bacillus thuringiensis TS2 was urged to test furthermore especially its formulation with based formula tapioca powder, peat and bulk. The most effective storage time also need to test. Result showed that all rhizobacterial formula had ability to decrease incidence of bacterial pustule disease compared to control. Moreover, all the three formula could increase plant growth, total of leaves, total of branch and yields. Flowering time was also advanced by 1-8 days compared to control. Decreasing of disease rate and increasing of plant growth rate variated between different formulations.
Penapisan Isolat Rizobakteri Indigenos untuk Pengendalian Ganoderma boninense pada Bibit Kelapa Sawit (Elaeis guineensis Jacq.)
Yanti, Yulmira;
Arnetti, Arnetti;
Rifai, Imam
Jurnal Agro Industri Perkebunan Volume 7 No. 2, Oktober 2019
Publisher : Politeknik Negeri Lampung
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DOI: 10.25181/jaip.v7i2.1156
Basal stem rot (Ganoderma boninense) is one of the main diseases of oil palm. The objective of the research was to obtain indigenous rhizobacteria (RBI) isolate which have the ability increase growth and to control basal stem rot on oil palm seedlings in planta. Experimental research consists of 3 stages by using Completely Randomized Design (CRD). Indigenous rhizobacteria isolate testing as plant growth promoting rhizobacteria (PGPR) and to control of G. boninense on pre-nursery of oil palm consisted of 29 treatments (27 RBI isolates, without G. boninense inoculation as positive control, and G. boninense inoculation as negative control) with 5 replications each. Data were analyzed by variance, if the result significantly different, it was continued by using Least Significance Different (LSD) at 5% level. The results showed that two of the best isolates were RZ2E 2.1 and RZ2E 1.2 which were able to increase growth and were able to suppress the development of basal stem rot G. boninense.
Aktivitas Peroksidase Mutan Pisang Kepok dengan Ethyl Methane Sulphonate (EMS) secara In Vitro 1
Yanti, Yulmira
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.14.1.32-36
The activity of peroxidase were observed in leave of banana clone Kepok resulting by treatments EMS. The phenotype of peroxidase wasanalyzed from the banana clones resulting the induced by EMS and without induction after inoculated with pathogen Banana Blood deasesBacterium (BBD). The objectives of research are know variation of activity and band pattern of peroxidase. Induced mutation treatmentsconsist of control, 0.2% EMS for 1 and 3 hours, 0.5% for 1 and 3 hours, each treatments was provided five banana clones. The result showedthat variant value and coefisien variant of peroxidase activity in leaf tissue of clone treated by EMS increased compare to the control. Avariant control is 0.28 with coefisien variant is 29.92%, while variant value of treatment is 8.45 with coefisien variant is 75.75%.Appearance of peroxidase bands on clone resulting by EMS treatments were emerged four band pattern. The first and control band patternhas relative migration distance is 20 and 30, the second 15, 40 and 60, the third is 15, 35 and 50. There are four bands with different relativemigration distance that indicated polymorphic.
Perubahan Bentuk Planlet Pisang Raja Sereh Hasil Mutasi dengan Ethyl Methane Sulphonate (EMS) Secara In Vitro
Yanti, Yulmira;
Habazar, Trimurti;
', Mardinus;
', Mansyurdin
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau
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DOI: 10.31258/jnat.11.2.104-108
The shoot of “rajasereh” banana was treated by 0,2% and 0,5% of ethyl methane sulfonate (EMS), for 2 and 4 hoursthourgh in vitro. The results showed that treatment of EMS mutagen would be changed morphologycal characterseither in planlet. In planlet stage was obtained four morphololgycal variations. One of the variant have characterizedthe colour yellowish; the leaves and stem are yellowish: the leaves were small and spiral. The value of coefficientof variant for morfphologycal characters of planlet “raja sereh” banana increased compare to the control. Theextreme value of coefficient of variant in planlet stage was found the times shoots is 84,31%, while control that is8,24%. EMS mutagen could caused several planlets shorter, total of leaves could of shoot are mostly
INDIGENOUS RHIZOBACTERIA SCREENING FROM TOMATO TO CONTROL RALSTONIA SYZIGII SUBSP. INDONESIENSIS AND PROMOTE PLANT GROWTH RATE AND YIELD
Yanti, Yulmira;
Hamid, Hasmiandy;
Reflin, Reflin
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 2 (2018): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung
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DOI: 10.23960/j.hptt.218177-185
Indigenous rhizobacteria screening from tomato to control Ralstonia syzigii subsp. indonesiensis and promote plant growth rate and yield. Bacterial wilt is the most damaging vascular pathogen on tomato and many other crops in tropical, subtropical and warm temperate areas of the world which limits the production. Rhizobacteria have been concerned as potential biological control agents due to their ability to promote plant growth and health, and their role as antagonists of plant pathogens. The purpose of this research was to screen the best indigenous rhizobacteria (IRB) that able to control bacterial wilt disease and increase growth rate and yield of tomato plant. This research was conducted in 3 stages: (1) Isolation and selection of indigenous rhizobacteria as PGPR on tomato seedlings, consisted of 27 IRB isolates and a control, with triplications; (2) Selection of IRB isolates that control R. syzigii subsp. indonesiensis on tomato plants, which consisted of 8 treatments including 7 IRB and a control with 5 replications; (3) Characterization of IRB isolates ability to promote plant growth (indicated with IAA production & phosphate solubilizing). The variables observed were disease development, growth enhancement and IRB isolate ability to produce IAA and solubilize phosphate. The results showed that all IRB isolates were able to control bacterial wilt disease and increase the growth rate and yield of tomato. IR2.3.5, IR1.3.4 and IR1.4.2 were the best isolates in controlling R. syzigii subsp. indonesiensis and increasing the growth rate and yield by 81.25% and 68.72% respectively. All isolates showed various abilities to produce IAA, however, only isolates IR2.3.5 and IR1.3.4 that had abilities to solubilize phosphate.
PENERAPAN PENGGUNAAN Trichoderma sp UNTUK PENGENDALIAN PENYAKIT PADI DI NAGARI SIMABUR KECAMATAN PARIANGAN KABUPATEN TANAH DATAR
Yanti, Yulmira;
Hamid, Hasmiandy;
Nurbailis, Nurbailis;
Hermeria, Noveriza;
Tanjung, Megha Putri
Jurnal Hilirisasi IPTEKS Vol 4 No 4 (2021)
Publisher : LPPM Universitas Andalas
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DOI: 10.25077/jhi.v4i4.579
Nagari Simabur is one of the nagari which is included in the Pariangan District, Tanah Datar Regency with the main sector only in the agricultural sector with the main crop being lowland rice. The problem that is often faced by Simabur farmers is the problem of pests and diseases which are the cause of the low growth and yield of the lowland rice plant. One of the main components of the IPM program that can be used as a biological control agent is the use of Trichoderma indigenous. In addition to the use of biological agents, the system or method of planting also affects the production results of the cultivated plants. The purpose of this community service is to increase public knowledge about the use of Trichoderma which can increase the growth and production of rice plants with the jajar legowo system. The method used in this activity is structured training and direct demonstration in the field. The results obtained from this activity were the production of grain weight/plant clump treated with Trichoderma was higher than the control with a difference of 42.45 grams. The conclusion of this activity is that the growth and yield of plants that were applied with Trichoderma were higher than those that were not treated with Trichoderma (control). The use of rhizobacteria is highly recommended because in addition to being easy to obtain, it also provides a positive value for plant growth and is good for environmental technology around cultivated plants.
Penapisan Bakteri Endofit Akar Kedelai Secara in Planta untuk Mengendalikan Penyakit Pustul Bakteri
Trimurti Habazar;
Zurai Resti;
Yulmira Yanti;
Jumsu Trisno;
Afrika Diana
Jurnal Fitopatologi Indonesia Vol 8 No 4 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)
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DOI: 10.14692/jfi.8.4.103
Endophytic bacteria reside within plant hosts without causing disease symptoms. Bacterial endophytes promote plant growth and yield, suppress pathogens, may help to remove contaminants, solubilize phosphate, or contribute assimilable nitrogen to plants. The aim of this study were to obtain indigenous endophytic bacterial isolates, which have the ability to control bacterial pustule cause by Xanthomonas axonopodis pv. glycines and to increase growth and yield of soybean. The bacterial endophyte isolates were introduced as seed treatment (108 cfu/ml) and soil drench on 3 weeks old soybean seedling. Xanthomonas axonopodis pv. glycines were inoculated on one month old of soybean by pricking leaf methodes. The effect of bacterial endophyte on disease incidence, disease severity, plant growth and yield of soybean were evaluated. We have found two selected bacterial endophyte isolates from soybean’s rhizosphere (St4E1.1 and St1E1.1.), with the ability to control bacterial pustule and to increase growth and yield of soybean.Key words: bacterial endophyte, bacterial pustule, in planta technique, soybean
Formulasi Bakteri Endofit Akar Kedelai untuk Pengendalian Pustul Bakteri
Trimurti Habazar;
Zurai Resti;
Yulmira Yanti;
Sutoyo Sutoyo;
Imelda Imelda
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)
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DOI: 10.14692/jfi.11.2.51
Two isolates of bacterial endophyte from soybean root were found to be effective to control bacterial pustule caused by Xanthomonas axonopodis pv. glycines. Formulation of the bacterial isolates is required to maintain the effectivity of this bacterial isolates during storage, transportation and application. The aim of this research was to obtain the best carrier for formulation to maintain the effectivity of bacterial endophyte in storage to control bacterial pustule on soybean. Three kind of carrier agent was evaluated for formulation of bacterial endophyte i.e. peat soil, tapioca flour and coconut water + 1% palm oil. Each carrier agent was combined with 5 treatment of storage time i.e. 0, 1, 3, 5 and 7 weeks. Soybean plants was inoculated by. X. axonopodis pv. glycines 2 weeks after planting by rubbing bacterial suspension (106 cel mL-1) on lower surface of leaves. The results showed that all formulas of bacterial endophyte were able to suppress the bacterial pustule on soybean. The best formulations were bacterial endophyte in peat soil stored for 1 and 7 weeks (effectivity rate 79.85% and 77.02%) and coconut water + palm oil and storaged for 3 weeks (effectivity rate 77.46%).
Penapisan Isolat Rizobakteri Indigenos untuk Pengendalian Ganoderma boninense pada Bibit Kelapa Sawit (Elaeis guineensis Jacq.)
Yulmira Yanti;
Arnetti Arnetti;
Imam Rifai
Jurnal Agro Industri Perkebunan Volume 7 No. 2, Oktober 2019
Publisher : Politeknik Negeri Lampung
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DOI: 10.25181/jaip.v7i2.1156
Basal stem rot (Ganoderma boninense) is one of the main diseases of oil palm. The objective of the research was to obtain indigenous rhizobacteria (RBI) isolate which have the ability increase growth and to control basal stem rot on oil palm seedlings in planta. Experimental research consists of 3 stages by using Completely Randomized Design (CRD). Indigenous rhizobacteria isolate testing as plant growth promoting rhizobacteria (PGPR) and to control of G. boninense on pre-nursery of oil palm consisted of 29 treatments (27 RBI isolates, without G. boninense inoculation as positive control, and G. boninense inoculation as negative control) with 5 replications each. Data were analyzed by variance, if the result significantly different, it was continued by using Least Significance Different (LSD) at 5% level. The results showed that two of the best isolates were RZ2E 2.1 and RZ2E 1.2 which were able to increase growth and were able to suppress the development of basal stem rot G. boninense.
Formula padat Bacillus cereus STRAIN TLE1.1 untuk pengendalian penyakit busuk pangkal batang (Sclerotium rolfsii) pada tanaman tomat
Yulmira Yanti;
Hasmiandy Hamid;
Reflin Reflin;
Yaherwandi Yaherwandi;
Febri Yani Chrismont
Jurnal Agro Vol 8, No 2 (2021)
Publisher : Jurusan Agroteknologi
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DOI: 10.15575/14679
Penyakit utama tanaman tomat yaitu busuk pangkal batang yang disebabkan oleh Sclerotium rolfsii dapat menimbulkan kerugian mencapai 80-100%. Tujuan penelitian yaitu untuk mendapatkan formula padat Bacillus cereus strain TLE1.1 yang efektif untuk pengendalian penyakit busuk pangkal batang pada tanaman tomat. Penelitian bersifat eksperimen dengan mengamati kemampuan formula padat B.cereus strain TLE1.1 dalam pengendalian penyakit busuk pangkal batang dengan Rancangan Acak Lengkap yang terdiri atas 9 perlakuan dan 3 ulangan. Perlakuan terdiri atas kombinasi bahan pembawa formula padat yang terdiri atas limbah padat ampas tebu, ampas tahu dan tongkol jagung, fungisida serta kontrol. Masing-masing formula padat B. cereus strain TLE1.1 diintroduksi pada benih dan bibit tomat. Hasil penelitian menunjukkan bahwa hampir semua formula mampu menekan penyakit busuk pangkal batang tanaman tomat. Formula terbaik dalam menurunkan penyakit busuk pangkal batang pada tanaman yaitu formula ampas tahu dan ampas tahu + tongkol jagung. Main disease of tomato plant, namely stem rot caused by Sclerotium rolfsii which can cause losses up to 80-100%. The aim of the study was to obtain a solid formula of Bacillus cereus strain TLE1.1 which was effective for controlling stem rot disease in tomato plant. This research was an experimental study to know the ability of the solid formula of B. cereus strain TLE1.1 in controlling stem rot disease which was carried out in a completely randomized design consisting of 9 treatments and 3 replications. The treatment consisted of a combination of solid formula carriers consisting of sugarcane solid waste, tofu dreg and corncob, fungicides and controls. Each solid formula of B. cereus strain TLE1.1 was introduced into tomato seeds and seedlings. The results showed that almost all of the formulas were able to suppress stem base disease of tomato plants. The best formula that reduced stem rot in plants were the tofu dreg and tofu dreg + corncob formula.