First Record of Free-Living Nematode Mylonchulus hawaiiensis from Bondowoso Regency-Indonesia

Ankardiansyah Pandu Pradana (Department of Plant Protection, Faculty of Agriculture, University of Jember, Jember 68121, Indonesia)
Toyoshi Yoshiga (Department of Applied Biological Sciences, Saga University, Saga 840-8502, Japan)

Jurnal Sumberdaya Hayati
Vol. 9 No. 1 (2023): 2023

Monochids are predatory nematodes that can be found in various ecosystems. In this study, we analyzed monochids from the rhizosphere of Arabica coffee in Bondowoso Regency-Indonesia. The morphological analysis showed that the nematode belonged to the Mylonchulus hawaiiensis (Cassidy, 1931) species. The analysis results were also confirmed by a molecular analysis using DNA from a single nematode. The study also involved a polymerase chain reaction process using a forward primer SSU F07 (5’-AAA GAT TAA GCC ATG CAT G-3’) and reverse primer SSU R 81 (5’-TGA TCC WKC YGC AGG TTC AC-3’), which amplifies the small subunit (SSU) region of rDNA. Phylogenetic analysis of the sequence obtained formed a cluster with those of M. hawaiiensis found in GenBank. The sequences obtained had homology ranging from 99.64 to 99.70% compared with M. hawaiiensis sequences found in GenBank. This study is the first report of M. hawaiiensis from Indonesia.