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All Journal Jurnal Sumberdaya Hayati
Ankardiansyah Pandu Pradana
Department of Plant Protection, Faculty of Agriculture, University of Jember, Jember 68121, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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First Record of Free-Living Nematode Mylonchulus hawaiiensis from Bondowoso Regency-Indonesia Ankardiansyah Pandu Pradana; Toyoshi Yoshiga
Jurnal Sumberdaya Hayati Vol. 9 No. 1 (2023): 2023
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.9.1.17-23

Abstract

Monochids are predatory nematodes that can be found in various ecosystems. In this study, we analyzed monochids from the rhizosphere of Arabica coffee in Bondowoso Regency-Indonesia. The morphological analysis showed that the nematode belonged to the Mylonchulus hawaiiensis (Cassidy, 1931) species. The analysis results were also confirmed by a molecular analysis using DNA from a single nematode. The study also involved a polymerase chain reaction process using a forward primer SSU F07 (5’-AAA GAT TAA GCC ATG CAT G-3’) and reverse primer SSU R 81 (5’-TGA TCC WKC YGC AGG TTC AC-3’), which amplifies the small subunit (SSU) region of rDNA. Phylogenetic analysis of the sequence obtained formed a cluster with those of M. hawaiiensis found in GenBank. The sequences obtained had homology ranging from 99.64 to 99.70% compared with M. hawaiiensis sequences found in GenBank. This study is the first report of M. hawaiiensis from Indonesia.
Bioefficacy of Steinernema sp. as a Biocontrol Agent Against Spodoptera frugiperda Under Laboratory Conditions Wagiyana; Imielda Yuli Hartika Sari; Bakhroini Habriantono; Ankardiansyah Pandu Pradana
Jurnal Sumberdaya Hayati Vol. 12 No. 2 (2026): 2026
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.12.2.39-45

Abstract

Spodoptera frugiperda control in agricultural crops, particularly corn crops, is a major challenge. The bioefficacy of the entomopathogenic nematode Steinernema sp. in suppressing S. frugiperda larvae was studied in this work. The Steinernema sp. isolate was obtained from the Biological Control Agent Service Post in Sidodai, while the S. frugiperda isolate was propagated at the University of Jember's Plant Pest Laboratory. In bioefficacy evaluations, different doses of Steinernema sp. were applied to third-instar S. frugiperda larvae, and mortality rates, lethal time 50% (LT50), and infection rates were monitored over 120 hours. The findings indicated the ability of Steinernema sp. to cause mortality in S. frugiperda larvae. Mortality increased with time, showing effective pest suppression. Significant differences among Steinernema sp. doses indicated a density-dependent impact. Greater nematode populations resulted in lower LT50 values, indicating quicker mortality induction. Infection rates decreased over time at all doses, although the highest concentration of 1,000 IJ mL⁻¹ consistently had the highest infection rate. These data confirm the potential of Steinernema sp. as an environmentally friendly biological control agent for S. frugiperda in corn crops.
Co-Authors Bakhroini Habriantono Imielda Yuli Hartika Sari Toyoshi Yoshiga Wagiyana