Produksi Konsentrat Protein dari Ampas Kelapa (Cocos nucifera L.) Menggunakan NaOH dan (NH4)2SO4 : Production of Protein Concentrate from Coconut Pulp (Cocos nucifera L.) using NaOH and (NH4)2SO4

Hasdinda Hasdinda
Pasjan Satrimafitrah (Jurusan Kimia FMIPA Universitas Tadulako)
Syaiful Bahri (Jurusan Kimia FMIPA Universitas Tadulako)
Abd. Rahman Razak (Jurusan Kimia FMIPA Universitas Tadulako)
Dwi Juli Puspitasari (Jurusan Kimia FMIPA Universitas Tadulako)
Indriani (Jurusan Kimia FMIPA Universitas Tadulako)
Aini Auliana Amar (Jurusan Kimia FMIPA Universitas Tadulako)

KOVALEN: Jurnal Riset Kimia
Vol. 9 No. 1 (2023): April Edition

Coconut pulp wasted from processed coconut oil has a protein content of up to 18.20%. The potential coconut pulp protein can be processed in protein concentrate which has many benefits. This research was conducted to determine the effect of NaOH and (NH4)2SO4 concentrations in isolating protein and producing protein concentrates that have the highest yield and protein content. Isolation of coconut pulp protein was carried out using NaOH at several concentrations, namely 0, 0.2, 0.4, 0.6, 0.8, and 1 M, while the protein concentrate was prepared using the salting out method with a saturation level of the ammonium sulfate salt of 55%, 60%, and 65%. Analysis of protein content was carried out using the Kjeldahl method. The use of NaOH 0.2 M and (NH4)2SO4 with a saturation level of 60% was the chosen treatment with crude protein content and yield of 75.63% and 33.83%, respectively.