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All Journal The Journal of Pure and Applied Chemistry Research Biocelebes KOVALEN: Jurnal Riset Kimia al Kimiya : Jurnal Ilmu Kimia dan Terapan
Pasjan Satrimafitrah
Department Of Chemistry, Faculty Of Mathematics And Science, Tadulako University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology Medicine & Pharmacology

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ANALISIS KADAR α-TOKOFEROL (VITAMIN E) DALAM DAUN KELOR (Moringa oleifera Lam) DARI DAERAH PESISIR DAN PEGUNUNGAN SERTA POTENSINYA SEBAGAI ANTIOKSIDAN Khalil Mubarak; Hasnah Natsir; Abd. Wahid Wahab; Pasjan Satrimafitrah
KOVALEN: Jurnal Riset Kimia Vol. 3 No. 1 (2017): Edisi April
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (780.652 KB)

Abstract

Alpha-tocopherol, a major vitamin E component, functions as an antioxidant that neutralizes free radicals in a living organism. Moringa oleifera Lam. leaves are rich source of carotenoids and -tocopherol. This research was aimed to determine the concentration of α-tocopherol and examine the activity of moringa leaves (Moringa  oleifera L.) crude extracts as an antioxidant. Sample used in this research were juvenile and adult moringa leaves (Moringa oleifera L), which were taken from seashore of Barru regency and highland of Soppeng regency. Extraction of the leaves was carried out by reflux method using ethanol as a solvent, and then evaporated to produce concentrated ethanol extract. Analyzes were detected at wavelenght of 510.20 nm using UV-Vis Spectroscopy, the results indicated that concentration of α-tocopherol extracted from juvenile and adult moringa leaves from seashore area were 104.52 mg/100g and 11.419 mg/100g respectively, whilst the leaves from highland area gave a concentration of 134.4 mg/100g and 114.918 mg/100g. Next, we examined the antioxidant activity of each type of leaf using DPPH (1,1-diphenyl-2-picrylhydrazyl) methods at the wavelength of 515 nm. The IC50 results data showed that ethanol extract of juvenile and adult leaves from seashore area were 172.71 μg/mL and 258.92 μg/mL respectively, and categorized as weak antioxidant. Adversely, juvenile leaves from highland area gave a result of 97.79 μg/mL and categorized as strong antioxidant; while adult leaves from the same area gave a values of 143.14 μL/mL and categorized as medium antioxidant. Vitamin E and BHT were used as control and had higher values compared to all samples.Keywords: α-tocopherol, antioxidant, Moringa oleifera leaves, DPPH, UV-Vis Spectrophotometer.
PEMANFAATAN KULIT UBI KAYU SEBAGAI BIOSORBEN PADA PENJERNIHAN AIR DI KELURAHAN BALAROA Syaiful Bahri; Musafira Musafira; Jaya Hardi; Pasjan Satrimafitrah
KOVALEN: Jurnal Riset Kimia Vol. 3 No. 2 (2017): Edisi Agustus
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

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Abstract

Kajian mengenai penjerapan ion logam Fe dalam air tanah menggunakan biosorben kulit ubi kayu telah berhasil dilakukan. Tujuan dari kajian ini adalah untuk menentukan pengaruh biosorben kulit ubi kayu terhadap sejumlah ion Fe dengan modifikasi perangkat alat penjernihan. Air tanah diperoleh dari Kelurahan Balaroa, Kota Palu, Indonesia. Biosorben kulit ubi kayu dikombinasikan dengan arang dan kerikil dalam perangkat alat penjernihan. Hasil kajian menunjukkan bahwa air tanah hasil penyaringan lebih jernih dibandingkan sebelum perlakuan. Air tanah hasil penyaringan tidak menghasilkan endapan besi setelah penyimpanan selama dua minggu. Kata kunci: ion Fe, biosorben, kulit ubi kayu, air tanah
PENERAPAN METODE EKSTRAKSI PELARUT DALAM PEMISAHAN MINYAK ATSIRI JAHE MERAH (Zingiber officinale Var.Rubrum) Nina Rahmadani; Ruslan Ruslan; Pasjan Satrimafitrah
KOVALEN: Jurnal Riset Kimia Vol. 4 No. 1 (2018): Edisi April
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.365 KB) | DOI: 10.22487/kovalen.2018.v4.i1.10186

Abstract

Telah dilakukan penelitian tentang penerapan metode ekstraksi pelarut dalam pemisahan minyak atsiri jahe merah (Zingiber officinale Var.rubrum). Tujuan penelitian ini adalah untuk mengetahui rasio pelarut n-heksan terhadap serbuk jahe merah yang menghasilkan ekstrak minyak atsiri jahe merah dengan rendemen yang tinggi dan komponen senyawa minyak atsiri jahe merah.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakuan yang diterapkan adalah pengaruh rasio pelarut n-heksan terhadap serbuk jahe merah yang terdiri atas lima perbandingan rasio yaitu 1:7, 1:8, 1:9, 1:10, dan 1:11 (b/v) dengan dua kali pengulangan. Parameter yang diamati adalah rendemen ekstrak minyak atsiri jahe merah. Hasil penelitian menunjukkan bahwa rendemen ekstrak minyak atsiri jahe merah yang tertinggi tardapat pada rasio 1:11 sebesar 6,695%. Analisis GC-MS minyak atsiri jahe merah menunjukkan bahwa terdapat 6 komponen senyawa yang dihasilkan. Kata Kunci  :  Minyak Atsiri Jahe, Ekstraksi, Gas Cromatography-Mass Spektrofotometri (GC-MS)
KADAR LIKOPEN EKSTRAK KASAR BUAH TOMAT DALAM VCO (VIRGIN COCONUT OIL) PADA PERENDAMAN SUHU RUANG Enni Indriani; Ruslan Ruslan; Prismawiryanti Prismawiryanti; Pasjan Satrimafitrah
KOVALEN: Jurnal Riset Kimia Vol. 4 No. 2 (2018): Edisi Agustus
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.882 KB) | DOI: 10.22487/kovalen.2018.v4.i2.10226

Abstract

Likopen merupakan salah satu senyawa karotenoid yang dapat berperan sebagai antioksidan yang memiliki sifat larut dalam minyak karena bersifat lipofilik. Penelitian ini memproduksi likopen yang berasal dari ekstrak kasat buah tomat dengan merendamnya dalam VCO. Tujuan penelitian ini untuk mengetahui rasio terbaik dariekstrak kasar buah tomat terhadap VCO dalam menghasilkan likopen, dan juga waktu ekstraksi untuk menghasilkan konsentrasi likopen tertinggi dari ekstraksi atau perendaman ekstrak kasar buah tomat dalam  VCO pada suhu ruang. Rasio antara ekstrak kasar buah tomat dan VCO adalah 1:40, 2:40, 3:40, 4:40, 5:40 (b/v) dengan masa ekstraksi mencapai 7 minggu. Hasil penelitian menunjukkan rasio ekstrak likopen kasar buah tomat terbaik pada penelitian ini yaitu 5:40 dan waktu ekstraksi terbaik adalah pada minggu pertama, dengan kadar likopen tertinggi sebesar  0,283 g.Kata kunci: Ekstrak tomat, likopen, VCO, waktu perendaman
PENENTUAN SUHU DAN pH HIDROLISIS KITOSAN DARI CANGKANG KEONG SAWAH (Pila ampullacea) TERHADAP BERAT MOLEKUL HIDROLISATNYA Nurhaeni Nurhaeni; Angriani Sambali; Pasjan Satrimafitrah; Jusman Jusman
KOVALEN: Jurnal Riset Kimia Vol. 5 No. 1 (2019): Edisi April
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (815.085 KB) | DOI: 10.22487/kovalen.2019.v5.i1.11454

Abstract

Telah dilakukan penelitian tentang penentuan suhu dan pH hidrolisis kitosan dari cangkang keong sawah (Pila ampullacea) terhadap berat molekul hidrolisatnya dengan menggunakan enzim α-amilase. Penelitian ini bertujuan untuk menentukan suhu dan pH hidrolisis terbaik yang dapat menghasilkan hidrolisat kitosan dengan berat molekul yang rendah. Variasi suhu yang digunakan untuk menghidroisis kitosan dari keong sawah antara lain 300C, 400C, 500C, 600C dan 700C. Sedangkan pH hidrolisis yang diterapkan  untuk menghidrolisis kitosan dari cangkang keong sawah antara lain 4,5; 5,0; 5,5; 6,0 dan 6,5. Pengujian berat molekul hidrolisat kitosan dilakukan dengan menerapkan metode Mark-Houwink dan parameter yang diamati adalah berat molekul hidrolisat kitosan. Hasil penelitian menunjukan bahwa suhu optimum hidrolisis kitosan yaitu suhu 500C dan menurunkan berat molekul menjadi 14.488 g/mol. Sedangkan untuk pH optimum hidrolisis adalah pH 5,5 dan menurunkan berat molekul 12.850 g/mol.Kata Kunci : hidrolisat kitosan, cangkang keong sawah, enzim α-amilase
UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TANAMAN TEMBELEKAN (Lantana camara Linn) DARI BEBERAPA TINGKAT KEPOLARAN PELARUT Ikatami Putri Lestari; Mappiratu Mappiratu; Ruslan Ruslan; Pasjan Satrimafitrah
KOVALEN: Jurnal Riset Kimia Vol. 4 No. 3 (2018): Edisi Desember
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.534 KB) | DOI: 10.22487/kovalen.2018.v4.i3.11850

Abstract

Penelitian tentang ekstrak daun tembelekan menggunakan berbagai tingkat kepolaran sebagai antibakteri telah dilakukan. Penelitian ini bertujuan untuk mengetahui senyawa antibakteri dalam daun tembelekan (Lantana camara Linn) bersifat polar, semipolar atau nonpolar, serta daya hambat ekstrak daun tembelekan terhadap bakteri gram positif (Staphylococcus pyogenesis dan Micrococcus luteus) dan bakteri gram negative (Vibrio cholera dan Shigella dysenteriae). Metode ekstraksi yang digunakan dalam penelitian ini, yaitu metode maserasi dengan 3 tingkat polaritas pelarut yang dimulai dari pelarut nonpolar (n­-heksan), diikuti dengan pelarut semipolar (etil asetat) dan pelarut polar (etanol). Uji daya hambat ekstrak terhadap bakteri uji dilakukan menggunakan metode sumur difusi. Hasil penelitian menunjukkan bahwa ekstrak etanol memberikan daya hambat tertinggi pada gram postif (Staphylococcus pyogenesis dan Micrococcus luteus) dan bakteri gram negative (Vibrio cholera dan Shigella dysenteriae) berturut-turut 20,89, 12, 18,56, dan 5,33 mm serta menunjukkan bahwa daun tembelekan mengandung senyawa antibakteri yang bersifat nonpolar, semipolar, dan polar.Kata Kunci : Tembelekan, ekstrak, daya hambat, antibakteri.
STABILITAS EKSTRAK WARNA BUNGA ASOKA (Ixora javanica) BERDASARKAN VARIASI pH SELAMA MASA PENYIMPANAN ellditna jenianti; Nurhaeni; Pasjan Satrimafitrah; Dwi Juli Pusptasari
KOVALEN: Jurnal Riset Kimia Vol. 5 No. 2 (2019): Edisi Agustus
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (552.354 KB) | DOI: 10.22487/kovalen.2019.v5.i2.12820

Abstract

Research on the potential of Ashoka (Ixora javanica) flower as a natural food and beverage coloring agent in terms of pH stability during the storage period. This study aims to determine the stability of Asoka flower extract on the effect of pH during the storage period. The time used was 10 days and the variation of pH of 2,3,4,5 and 6, respectively. The results show that Asoka flower extract was stable at pH 2 after a 10-day storage period with degradation presentation of 36.21%, while Ashoka flower extract dyes were unstable at pH 6 after a 10-day storage period with degradation percentage is 78.02%. This proves that the different acidic atmosphere influences the stability of Ashoka flower extract. Keywords : Dyes, stability of Ashoka flowers, degradation
Aktivitas Antibakteri Ekstrak Metanol dari Kulit Batang Kelor (Moringa oleifera Lam.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli: Antibacterial Activity of Methanol Extracts from The Stem Bark of Moringa oleifera Lam. Against Staphylococcus aureus and Escherichia coli Nurul Cholifah; Ahmad Ridhay; Pasjan Satrimafitrah; Ruslan; Hardi Ys
KOVALEN: Jurnal Riset Kimia Vol. 6 No. 1 (2020): Edisi April
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2020.v6.i1.12854

Abstract

Antibacterial activity of Moringa oleifera Lam. stem bark has been tested against Staphylococcus aureus and Escherichia coli. The extraction of Moringa oleifera Lam. stem bark was used maceration method with methanol solvent and has obtained extract yield of 6.1%. The antibacterial activity test of Moringa oleifera stem bark extracts used a well diffusion method. The concentration of Moringa oleifera stem bark extract was varied to four concentrations of 1% 2% 3% 4% (w/v). The inhibition zone of methanol extract of Moringa oleifera stem bark against Staphylococcus aureus at extract concentrations of 1%, 2%, 3%, and 4% was 10.08 mm, 11.8 mm, 15.00 mm, and 17.02 mm, respectively. The methanol extract of Moringa oleifera stem bark at concentrations of 1%, 2%, 3%, and 4% could also inhibition of Escherichia coli growth with inhibition zone of 14.01 mm, 16.50 mm, 17.09 mm, and 17.10 mm, respectively. Keywords: Moringa Oleifera Lam., Antibacterial, Staphylococcus aureus, Escherichia coli
Viskositas dan Aktivitas Antibakteri Sabun Cair Berbasis VCO dengan Penambahan Ekstrak Etanol Daun Kelor (Moringa oleifera) Terhadap Bakteri Patogen: Viscosity and Antibacterial Activity of VCO-Based Liquid Soap with Addition of Ethanol Extract of Moringa Leaf (Moringa oleifera) Against Pathogenic Bacteria Pasjan Satrimafitrah; Mohamad Afdal; Jusman; Abd. Rahman Razak; Ahmad Ridhay; Nov Irmawati Inda
KOVALEN: Jurnal Riset Kimia Vol. 8 No. 1 (2022): April Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2022.v8.i1.15846

Abstract

This research was conducted by making VCO-based liquid soap with the addition of ethanol extract from Moringa leaves (Moringa oleifera). The purpose of this research was to determine the mass ratio of ethanol extracts of Moringa leaves and Virgin Coconut Oil (VCO) which produces soap with the highest antibacterial activity against tested bacteria. The other purpose is to determine the effect of the mass ratio against the viscosity of liquid soap. The research design used was a Completely Randomized Design (CRD) with the independent variables being the mass ratio of Moringa leaf ethanol extract and VCO which consists of five levels of 0:75 (F1), 1.5:73.5 (F2), 3:72(F3), and 4.5:70.5(F4) (w/w). The soap produced was tested for antibacterial activity againts Staphylococcus aureus, Escherichia coli, and Staphylococcus epidermidis using agar diffusion method as well as viscosity tests. The results showed that the antibacterial activity of liquid soap tends to be higher with increasing levels of ethanol extract of Moringa leaves. The liquid soap formula with the ratio of Moringa leaf ethanol extract: VCO of 3:72 (w/w) (Formula F3) had relatively higher antibacterial activity against the three tested bacteria. The inhibition zones against Staphylococcus aureus, Escherichia coli, and Staphylococcus epidermidis were 22.302 mm, 33.230 mm, and 36.490 mm, respectively, while the viscosity value of liquid soap was 47.433 cP.
Analisis in-Silico pGEM-GLL Recombinan Glukoamilase Saccharomycopsis fibuligera R64 Pasjan Satrimafitrah
Biocelebes Vol. 4 No. 1 (2010)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Saccharomycopsis fibuligera is yeast with high amylolitic activity. The local strain, S. fibuligera R64 has been reported to produce starch hydrolyzing enzymes, -amylase dan glucoamylase. Glucoamylase is one of the extracellular enzymes that enable to breakdown starch in starch processing, thus has potential to be developed and applied in starch processing industry, such as glucose syrup production. Wild type microorganism-producing glucoamylase normaly express low level of enzyme, while high level production and low cost enzymes are needed in many industries. To obtain strains with superior properties, a recombinant DNA is used and Pichia pastoris is a suitable expression host for producing glucoamylase at high level. Gene encoding glucoamylase S. fibuligera R64 (GLL) has been amplified and cloned into pGEM-T vector. A set of primers (Glu-F and Glu-R) was designed based on the published nucleotide sequence (Acc. No M17355). Restriction analysis of pGEM-GLL by using EcoRI and XhoI showed a ~4.500 kb DNA fragment which confirmed the size of pGEM-T vector containing GLL. This research aimed to analyze pGEM-GLL S. fibuligera R64 in silico. Gene alignment of GLL S. fibuligera R64 with GLU1 S. fibuligera showed 99 % homology. Based on in silico translation analysis, there were several nucleotide difference compared to the published sequence which result in amino acid differences, namely E27D, N46D, dan A376V. Hence it is possible that S. fibuligera R64 has different properties from other known S. fibuligera. Key words: Glucoamylase, S. fibuligera, E. coli TOP10F’, PCR, in silico analysis.
Co-Authors Abd. Rahman Razak Abd. Rahman Razak Abd. Wahid Wahab Ahmad Ridhay Ahmad Ridhay Aini Auliana Amar Angriani Sambali Dwi Juli Puspitasari Dwi Juli Pusptasari ellditna jenianti Enni Indriani Fridian Aprilia, Natasya Hardi Ys Hasdinda Hasdinda Hasnah Natsir Ikatami Putri Lestari Inda Hastuti N Naser Inda, Nov Irmawati Indriani Indriani Indriani Jaya Hardi Jaya Hardi Jusman, Jusman Khairuddin Khalil Mubarak Mappiratu Mappiratu Mohamad Afdal Musafira Musafira Ni Kadek Atmiyanti Ni Ketut Sumarni Nina Rahmadani Nov Irmawati Inda Nurhaeni Nurhaeni Nurhaeni Nurhaeni Nurhaeni NURUL CHOLIFAH Prismawiryanti Prismawiryanti Rismawaty Sikanna Ruslan Ruslan Ruslan Sarapun, Veky Shiomori, Koichiro SITTI HADIJAH SABARWATI Syaiful Bahri Syaiful Bahri Syaiful Bahri