ANTIBACTERIAL ACTIVITY PROFILE OF MANGROVE ENDOPHYTIC FUNGI ISOLATED FROM BERAU REGENCY, INDONESIA

Ferdina Tri Laksmita (Food Science Study Program, Graduate School, IPB University, Bogor 16680, Indonesia)
. Sukarno (Dept. of Food Science & Technology, Faculty of Agricultural Engineering & Technology, IPB University, Bogor 16680, Indonesia)
Slamet Budjianto (Dept. of Food Science & Technology, Faculty of Agricultural Engineering & Technology, IPB University, Bogor 16680, Indonesia)
Siti Irma Rahmawati (Research Center for Biotechnology, Indonesian Institute of Science, Cibinong Science Center, Cibinong, Bogor 16911, Indonesia)
Rikno Harmoko (Research Center for Biotechnology, Indonesian Institute of Science, Cibinong Science Center, Cibinong, Bogor 16911, Indonesia)
Fauzia Nurul Izzati (Research Center for Biotechnology, Indonesian Institute of Science, Cibinong Science Center, Cibinong, Bogor 16911, Indonesia)
Saiful Bachri (Southeast Asian Regional Centre for Tropical Biology (SEAMEO BIOTROP), Tajur, Bogor 16134, Indonesia)
. Anidah (Southeast Asian Regional Centre for Tropical Biology (SEAMEO BIOTROP), Tajur, Bogor 16134, Indonesia)
Salcia Inka Nelanda (Department of Biology, College of Arts and Science, Boston University, Boston, Massachusetts 02215, USA)
Armaiki Yusmur (Southeast Asian Regional Centre for Tropical Biology (SEAMEO BIOTROP), Tajur, Bogor 16134, Indonesia)
. Aslan (Innovation Center for Tropical Science (ICTS), Sukadamai Green Residence D2, Tanah Sareal, Bogor 16165, Indonesia)
Muhammad Ilman (Yayasan Konservasi Alam Nusantara (The Nature Conservancy partner in Indonesia), Graha Iskandarsyah 3rd Floor, Iskandarsyah Raya Street No. 66C, Kebayoran Baru, Jakarta 12160, Indonesia)

Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Vol. 9 No. 1 (2022): June 2022

Mangrove endophytic fungi have the potential to produce secondary metabolites with antibacterial properties. This study aimed to obtain endophytic mold isolates associated with mangrove plants and their antimicrobial activity. Seventeen endophytic molds were isolated from 7 mangrove species originating from Berau, East Kalimantan, Indonesia. Three extracts from the mold isolates OJ-B, OJ-Bu, and 6Xg-Bh showed the highest antibacterial activity with an inhibition zone of 9.63–25.4 mm and with MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values ??of around 0.625–5 and 2.5–10 mg mL–1, respectively. LC-MS (liquid chromatography-mass spectrometry) analysis identified several compounds with antibacterial potential, namely beauvericin, choline, nicotinic acid, pyridoxine, quinoline, cyclo (phenylalanyl-prolyl), fusarin c, oleamide and borrelidin. The 3 species were molecularly identified as Diaporthe phaseolorum, Fusarium proliferatum and Phomopsis sp. Thus, the endophytic molds from several of the mangrove species produced bioactive compounds as antibacterial.  Kapang endofit mangrove berpotensi menghasilkan metabolit sekunder yang bersifat antibakteri. Penelitian ini bertujuan mendapatkan isolat kapang endofit yang berasosiasi dengan tanaman mangrove beserta aktivitas antimikrobanya. Tujuh belas kapang endofit diisolasi dari 7 spesies mangrove asal Berau, Kalimantan Timur, Indonesia. Tiga ekstrak dari isolat kapang OJ-B, OJ-Bu, dan 6Xg-Bh memiliki aktivitas antibakteri tertinggi dengan zona hambat 9.63–25.4 mm dan dengan nilai MIC dan MBC masing-masing sekitar 0.625-5 dan 2.5–10 mg mL–1. Analisis LC-MS menunjukkan beberapa senyawa dengan potensi antibakteri yaitu beauvericin, choline, nicotinic acid, pyridoxine, quinoline, cyclo (phenylalanyl-prolyl), fusarin c, oleamide dan borrelidin. Uji molekuler menunjukkan 3 spesies terindentifikasi sebagai Diaporthe phaseolorum, Fusarium proliferatum dan Phomopsis sp.  Hasil menunjukkan kapang endofit dari beberapa spesies mangrove tersebut mampu menghasilkan senyawa bioaktif sebagai antibakteri.