Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

1.745

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia Biota Jurnal Bioteknologi & Biosains Indonesia (JBBI) JURNAL PANGAN

Sukarno Sukarno

Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680

Author-ID : 2465380

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

Published : 15 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

Endophytic Fungi Associated With Turmeric (Curcuma longa L.) Can Inhibit Histamine-Forming Bacteria in Fish Eris Septiana; Nampiah Sukarno; . Sukarno; Partomuan Simanjuntak
HAYATI Journal of Biosciences Vol. 24 No. 1 (2017): January 2017
Publisher : Bogor Agricultural University, Indonesia

Turmeric (Curcuma longa L.) is a medicinal plant that is commonly used as spice and preservative. Many types of endophytic fungi have been reported as being associated with medicinal plants and able to synthesize secondary metabolites. In this study, endophytic fungi were isolated from all plant parts of turmeric plants. Identification of the endophytic fungi was done using morphological characteristics and sequencing of the internal transcribed spacer (ITS) region of ribosomal DNA. The dual culture method was used for screening antibacterial activity of the endophytic fungi against Morganella morganii, a common histamine-producing bacteria. The disc diffusion method was used to test the ability of water fractions of selected endophytic fungi to inhibit M. morganii growth. Two-dimensional thin layer chromatography was used to determine the fungal extract inhibition activity on histamine formation. In total, 11 endophytic fungi were successfully isolated and identified as Arthrobotrys foliicola, Cochliobolus kusanoi, Daldinia eschscholzii, Fusariumoxysporum, Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Phanerochaete chrysosporium, and Phaeosphaeria ammophilae. Five isolates showed inhibition activity against M. morganii in the dual culture tests. Based on the disc diffusion assay, A. foliicola and F. verticillioides inhibited the growth of M. morganii as a histamine-producing bacteria, and inhibiting histamine formation in fish. The best effects in inhibiting growth of the histamine-producing bacteria and histamine formation inhibition in fish were produced with F. verticillioides water fraction at 0°C incubation.

Karakterisasi Sifat Fisikokimia Sereal Berbasis Tepung Beras Merah Pecah Kulit Sukarno Sukarno; Nova Kushandita; Slamet Budijanto
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 1 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

The purpose of this study was to formulate cereals from red rice, red beans, and sesame into the right food as a food consumed at breakfast and characterization of its physical chemistry. The cereal was made by extrusion method using an extruder with a temperature of 130°C and a rotating speed of auger, screw, and cutter 50 Hz. This study used a completely randomized design with 9 formulas. Based on the Bayes method, the best formula was F8 with a composition of brown red rice (85%), red beans (10%), and sesame (5%) cereals contained a total phenol compound of 0.10± 0.01 mg GAE/g, water content of 7.35±0.58%, ash content of 0.02±0.00%, protein content of 10.85±0.29%, fat content of 1.77±0.08, and carbohydrate content of 80.01±0.79%. Keywords: breakfast cereal, brown rice, red beans, red rice, sesame

Pengembangan Sereal Sarapan Tersubstitusi Bekatul dan Tepung Pisang Sukarno Sukarno; Dwyana Izza Augusta; Azis Boing Sitanggang; Afwa Nururrahmah Munawaroh; Slamet Budijanto
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 1 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.1.76

Rice bran and banana flour contain nutrients and bioactive compounds that have potential to be used for the development of functional food, such as breakfast cereal. The study is aimed to develope functional breakfast cereal from corn flour (54%, 59%, 64%), rice bran (20%), and banana flour (10%) (raja bandung and kepok) with the addition of coconut sugar (0 %, 5 %, 10%) using twin-screw extruder. The best-accepted formula based on sensory test was cereal with the substitution of 0% coconut sugar and 10% raja bandung banana flour. The breakfast cereal has moisture content of 10.38 ± 0.01%, ash content of 3.36 ± 0.00%, protein content of 8.92 ± 0.11%, fat content of 6.14 ± 0.07%, carbohydrate content of 81.58 ± 0.18%, and dietary fiber content of 13.22 ± 0.46% which can be claimed as high-fiber foods. It contained a total phenol content of 74.13 ± 0.87 mg GAE / 100 g, antioxidant activity of 40.67 ± 1.71 mg AEAC/ 100 g, and IC50 of 84.48 ± 0.9 mg/mL. Saturated fatty acids in breakfast cereal were dominated by palmitic acid (14.22 ± 0.17%), while for unsaturated fatty acids were dominated by oleic acid (30.76 ± 0.04%) and linoleic acid (45.40 ± 0.03%). Keywords: banana flour, breakfast cereal, functional food, rice bran

PENGARUH FRAKSI NONPROTEIN KACANG KOMAK (Lablab purpureus (L.) Sweet) TERHADAP KADAR GLUKOSA DARAH DAN MALONALDEHIDA TIKUS DIABETES [Effect of Nonprotein Fraction of Hyacinth Bean (Lablab purpureus (L.) Sweet) Diet on Glucose and Malonaldehyde Serum of Arif Hartoyo; Sukarno .; Erma Rohmawati
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.135 KB)

The hypoglycemic response to Lablab nonprotein fraction (NPK) was evaluated in alloxan-induced diabetic rats. The objectives of this research were to evaluate the effect of Lablab nonprotein fraction diet on the blood glucose concentration and the lipid peroxide level of alloxan-induced diabetic rats. Two months old male Sprague Dawley rats were divided into 4 groups, each group contained of 5 rats. Three groups were diabetic rats induced by alloxan injection (110 mg/kg of body weight by intra-pheritonial injection) while one group was a control,normal rat. The experiment groups were (1) normal (group I), (2) diabetic (group II), (3) diabetic+cholesteol 0.5% (control group, group III), and (4) diabetic+cholesterol 0.5% + lablab NPK (group IV). The concentration of rat’s blood glucose were periodically measured during diet intervenion (day 0,14,27, and 42). The Lipid peroxide was evaluated as the concentration of malonaldehyde (MDA) both in serum and liver of the rats by Thiobarbituric Acid Reactivity Test methode. The result demonstrated that after 42 days of intervention, the Lablab nonprotein diet decreased the blood glucose concentrations from 444.00 + 143.00 mg/dl to 310.50 +111.40 mg/dl (30%), while control group has decreased the blood glucose concentration from 458.00 +164.99 mg/dl to 455.33 + 81.95 mg/dl (0.6%). Lablab nonprotein diet significantly (P<0.05) reduced the concentration of blood glucose as compared to the control group. However, Lablab nonprotein fraction diet did not give a significant diferrence on the level of serum MDA and liver MDA as compared to the control group.

KARAKTERISTIK FUNGSIONAL PROTEIN MISELIUM JAMUR TIRAM MERAH MUDA DAN MERANG [Functional Characteristics of Protein Mycelium of Pink Oyster and Paddy Straw Mushrooms] - Sukarno; Nadia T. Hendartina; Dedi Fardiaz; Nampiah Sukarno
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

KARAKTERISTIK FUNGSIONAL PROTEIN MISELIUM JAMUR TIRAM MERAH MUDA DAN MERANG [Functional Characteristics of Protein Mycelium of Pink Oyster and Paddy Straw Mushrooms]Sukarno1)*, Nadia T. Hendartina1), Dedi Fardiaz1) dan Nampiah Sukarno2)1) Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor2) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor Diterima 12 Maret 2014 / Disetujui 13 Juni 2014ABSTRACT Mycelium of mushroom contained high protein, which determined its functional characteristics such as water holding capacity (WHC), oil holding capacity (OAC), emulsion stability, and gel formation. This study aimed to determine the protein functional properties of Pleurotus flabellatus and Volvariella volvacea mycelia. Information obtained can be used to increase utilization of the mycelia as source of food. Mycelia biomass were obtained by growing the fungal cultures in Potato Dextrose Broth (PDB) on shaker at 100-150 rpm. Mycelia were harvested three times at 7, 8, and 9-days after inoculation for measuring their protein contents by kjehdahl method. Functional properties of mycelium protein measured were WHC, OAC, emulsion stability, and gel formation by folding test method. Based on the analysis of protein content in dry weight basis, 8-day old P. flabellatus and V. volvacea mycelia produced the highest protein contents with the value were 31.72 and 19.98%, respectively. Further analysis of protein functional properties showed that P. flabellatus mycelium had 10.38% of WHC, 0.52 mL/g of OAC, 57.14% of emulsion stability and gel strength level with the valueof 2, whereas the V. volvacea mycelium had 15.89% of WHC, 0.80 mL/g of OAC, 48.69% of emulsion stability, and did not form a gel. Protein functional properties of P. flabellatus were better than that of V. volvacea mycelium in terms of protein content, emulsion stability, and gel formation.

AKTIVITAS PENGHAMBATAN EKSTRAK BERBAGAI JENIS BAWANG TERHADAP PEMBENTUKAN BLACKSPOT PADA UDANG VANAME Tatty Yuniati; Sukarno Sukarno; Nancy Dewi Yuliana; Slamet Budijanto
Jurnal Teknologi dan Industri Pangan Vol. 29 No. 1 (2018): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Blackening is a problem occurring in crustaceans, such as whiteleg shrimps (Litopenaeus vannamei), during post-mortem storage. Although blackspot seems to be harmless to consumers, it decreases the product’s market value, consumer’s acceptance and causes a considerable financial loss. The objective of the study was to determine the best Allium extracts from five Allium types with the ability to inhibit blackspot and apply the extracts on whiteleg shrimp. The five types of Allium were red onion (Allium cepa L. var Aggregatum), garlic (Allium sativum), yellow onion (Allium cepa Linneaus), leek (Allium fistulosum) and chives (Allium schoenoprasum L). Extraction of Allium was conducted using 80% methanol as the solvent. The dry extracts were tested in vitro using a commercial polyphenol oxidase (PPO) enzyme and a melanosis test was conducted on whiteleg shrimp treated with the extracts and stored at 0°C for 10 days. The results showed that the highest inhibition of blackspot formation was obtained by red onion extract at 69.79±1.91% and the lowest was by garlic extract at 14.06±1.41%. The shrimps dipped in red onion extract had the lowest melanosis values and browning index values at 5.1±0.8 and 16.76±0.94, respectively. Red onion extract had the best ability to inhibit blackspot and has the potential to be used in shrimp.

KARAKTERISTIK MUTU FISIK TEKWAN KERING DENGAN RASIO IKAN BERBEDA Muhammad Irfan Febriansyah; Sukarno Sukarno; Dedi Fardiaz
Jurnal Teknologi dan Industri Pangan Vol. 30 No. 1 (2019): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Dried tekwan is a traditional restructured-fish product of South Sumatera processed by convectional drying. The study aimed to determine the effect of fish and tapioka ratio and tekwan’s length on the physical quality of the dried tekwan. The physical quality of dried tekwan made with different ratio of fish and tapioca ratio (1:1, 1.5:1 and 2:1 (w:w)) and length 1, 2, and 3 cm were investigated. The physical quality evaluated were the degree of whiteness, rehydration ratio, water holding capacity (WHC) and microstucture. The drying profile and proximate composition were also evaluated. There was no difference in the rate of drying of different fish:tapioca ratio as well a different tekwan length when free water evaporation was progressing. However, difference in drying rate was observed when the water content reached the equilibrium. Ratio 2:1 of fish and tapioca and 2 cm length of tekwan was found the to be best treatment and resulting in dried tekwan with moisture content 10.22%, ash 1.9%, protein 27.9%, fat 1.38%, carbohydrate 58.6%, whiteness 45.96% and expressible moisture content 2.06%. Microstructure of dried tekwan with 2:1 ratio showed more compact structure and slight damage than 1.5:1 ratio. However, dried tekwan with 2:1 ratio still had lower rehydration properties than other treatment.

PERBAIKAN SIFAT FUNGSIONAL TEKNIS TEPUNG BIJI KELOR (MORINGA OLEIFERA) DENGAN PERKECAMBAHAN Candytias Puspitasari; Sukarno Sukarno; Slamet Budijanto
Jurnal Teknologi dan Industri Pangan Vol. 30 No. 2 (2019): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Germination constitutes one of bio-processing techniques and has attracted a tremendous attention due to its advantageous effects on the improvement of technical functional properties of grains. Mean-while, blanching has been used as the complementary treatment in germination. The aim of this study was to evaluate the changes in the functional properties of Moringa oleifera seed flour due to germination and blanching that affect the characteristics of the resulting food the products. Germination was performed at four levels of incubation time (0, 48, 72, and 96 hours) at room temperature in the dark room and two levels of blanching time (0 and 30 second). The functional properties analyzed consisted of bulk density, flour solubility, water and oil absorption, and capacity and stability of emulsion. The results of this study showed that germinated Moringa seed flour had significantly better technical functional characteristics than the ungerminated ones, i.e. bulk density (0.33-0.43 g/mL), flour solubility (16.82-21.00 g/g), water absorp-tion capacity (0.93-0.99 g water/g), oil absorption capacity (1.18-1.58 mL oil/g), and emulsion capacity (88.80-91.70%). Blanching decreased the functional properties of the flour. The statistical analysis showed that there was no significant interaction between two factors in all parameters. This study shows that ger-mination is a good method to improve the functional properties of Moringa seed flour, but blanching dec-reases them. Germination without blanching is recommended to improve the technical functional charac-teristics of the flour.

Karakterisasi Sifat Fisikokimia dan Fungsional Beras Pecah Kulit Berpigmen dan Tanpa Pigmen Budi suarti; sukarno sukarno; ardiansyah ardiansyah; slamet budijanto
JURNAL PANGAN Vol. 30 No. 1 (2021): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v30i1.515

Beras pecah kulit kaya dengan kandungan gzi dan komponen bioaktif yang bermanfaat terhadap kesehatan. Penelitian ini bertujuan untuk mengevaluasi perbedaan karakterisasi sifat fisikokimia, fungsional, dan aktivitas penghambatan α-glukosidase beras pecah kulit non pigmen (Mentik wangi susu), dan pigmen (Cempo merah dan Jowo melik). Beras pecah kulit yang digunakan mentik wangi susu, cempo merah dan jowo melik, analisis yang dilakukan yaitu warna, proksimat, amilosa, total fenol, antosianin, antioksidan, dan inhibisi α-glukosidase. Hasil dari penelitian ini menunjukkan bahwa kandungan total senyawa fenolik dan antosianin, lebih tinggi pada beras pecah kulit berpigmen daripada beras non pigmen dengan hasil tertinggi pada varietas Jowo melik. Aktivitas antioksidan varietas Cempo merah lebih tinggi dibandingkan varietas Jowo melik, dan Mentik wangi susu. Parameter nilai inhibisi α-glukosidase (IC50), varietas Cempo merah memiliki aktivitas inhibitor α-glucosidase yang lebih tinggi daripada varietas Jowo melik, dan Mentik wangi susu dengan nilai masing-masing yaitu 231,66 ppm, 1175,89 ppm, dan 46314 ppm. Penelitian ini menunjukkan bahwa beras merah varietas Cempo merah berpotensi untuk dijadikan pertimbangan dalam pengembangan produk pangan bagi penderita diabetes.

ANTIBACTERIAL ACTIVITY PROFILE OF MANGROVE ENDOPHYTIC FUNGI ISOLATED FROM BERAU REGENCY, INDONESIA Ferdina Tri Laksmita; . Sukarno; Slamet Budjianto; Siti Irma Rahmawati; Rikno Harmoko; Fauzia Nurul Izzati; Saiful Bachri; . Anidah; Salcia Inka Nelanda; Armaiki Yusmur; . Aslan; Muhammad Ilman
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Mangrove endophytic fungi have the potential to produce secondary metabolites with antibacterial properties. This study aimed to obtain endophytic mold isolates associated with mangrove plants and their antimicrobial activity. Seventeen endophytic molds were isolated from 7 mangrove species originating from Berau, East Kalimantan, Indonesia. Three extracts from the mold isolates OJ-B, OJ-Bu, and 6Xg-Bh showed the highest antibacterial activity with an inhibition zone of 9.63–25.4 mm and with MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values ??of around 0.625–5 and 2.5–10 mg mL–1, respectively. LC-MS (liquid chromatography-mass spectrometry) analysis identified several compounds with antibacterial potential, namely beauvericin, choline, nicotinic acid, pyridoxine, quinoline, cyclo (phenylalanyl-prolyl), fusarin c, oleamide and borrelidin. The 3 species were molecularly identified as Diaporthe phaseolorum, Fusarium proliferatum and Phomopsis sp. Thus, the endophytic molds from several of the mangrove species produced bioactive compounds as antibacterial. Kapang endofit mangrove berpotensi menghasilkan metabolit sekunder yang bersifat antibakteri. Penelitian ini bertujuan mendapatkan isolat kapang endofit yang berasosiasi dengan tanaman mangrove beserta aktivitas antimikrobanya. Tujuh belas kapang endofit diisolasi dari 7 spesies mangrove asal Berau, Kalimantan Timur, Indonesia. Tiga ekstrak dari isolat kapang OJ-B, OJ-Bu, dan 6Xg-Bh memiliki aktivitas antibakteri tertinggi dengan zona hambat 9.63–25.4 mm dan dengan nilai MIC dan MBC masing-masing sekitar 0.625-5 dan 2.5–10 mg mL–1. Analisis LC-MS menunjukkan beberapa senyawa dengan potensi antibakteri yaitu beauvericin, choline, nicotinic acid, pyridoxine, quinoline, cyclo (phenylalanyl-prolyl), fusarin c, oleamide dan borrelidin. Uji molekuler menunjukkan 3 spesies terindentifikasi sebagai Diaporthe phaseolorum, Fusarium proliferatum dan Phomopsis sp. Hasil menunjukkan kapang endofit dari beberapa spesies mangrove tersebut mampu menghasilkan senyawa bioaktif sebagai antibakteri.

Co-Authors . Anidah . Aslan Adham Prayudi Afwa Nururrahmah Munawaroh Ahmad Fahrizal Ardiansyah, Ardiansyah Arif Hartoyo Armaiki Yusmur Azis Boing Sitanggang Budi Suarti Candytias Puspitasari David Yudianto Dedi Fardiaz Dwyana Izza Augusta Dyah Ayu Rakhmayeni Eko Hari Purnomo Eris Septiana Erma Rohmawati Fauzia Nurul Izzati Febriansyah, Muhammad Irfan Ferdina Tri Laksmita Ida Ayu Iska Rakhmawati Meta Mahendradatta Metusalach Metusalach Muhammad Ilman Muhammad Nur Nadia T. Hendartina Nampiah Sukarno Nancy Dewi Yuliana Nova Kushandita Nurfaidah Nurfaidah Partomuan Simanjuntak Rahmawati, Siti Irma Ratih Dewanti -Hariyadi Rikno Harmoko Riza Firmansyah Saiful Bachri Salcia Inka Nelanda Slamet Budijanto Slamet Budjianto Sufardin Sufardin Sulfiana Sulfiana Tatty Yuniarti Tatty Yuniati Utut Widyastuti Suharsono Wendi Nurul Fadillah

Title

Found 15 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 15 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 15 Documents
Search