JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)
Vol. 5 No. 1 (2023): Journal of Chemical Natural Resources

Analysis of Fatty Acid Composition Using GC-MS and Antibacterial Activity Test of n-Hexane Extract from Jengkol Seeds (Pithecellobium lobatum Benth.)

Marpaung, Lamek (Unknown)
Siahaan, Surya Graha P. (Unknown)



Article Info

Publish Date
18 May 2023

Abstract

Analysis of fatty acid composition using GC-MS and antibacterial activity test of n-hexane extract from Jengkol seeds (Pithecellobium lobatum Benth) was performed with the maceration method to obtain n-hexane extract of Jengkol seeds. The n-hexane extract of Jengkol seeds was first esterified using methanol (1.7 ml): 98% H2SO4 (0.3 ml): Chloroform (2 ml), then analyzed its fatty acid composition by GC-MS method. The results of fatty acid composition analysis found in Jengkol seeds are saturated fatty acids, namely: Margaric Acid (1.10%), Palmitate Acid (2.64%), stearic acid (12.01%), and unsaturated fatty acids, namely: Linolelaidate acid (10.02%) %), and oleic acid (74.23%). The antibacterial activity test against Escherichia coli and Staphylococcus aureus using n-hexane extract of Jengkol seed was done by agar diffusion method. The concentration variations were 50 mg/ml, 150 mg/ml, and 250 mg/ml. From the results of this test, n-hexane Jengkol seeds have an antibacterial activity that can inhibit bacterial growth but is weak (≤14 mm).

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Journal Info

Abbrev

JCNaR

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

Description

Journal of Chemical Natural Resources (JCNaR) is a peer-reviewed biannual journal (February and August) published by TALENTA as an open access journal. The aim of the journal is to provide a medium to exchange ideas and information related to research and knowledge in disciplines of organic ...