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All Journal Chimica et Natura Acta FITOFARMAKA: Jurnal Ilmiah Farmasi JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)
Lamek Marpaung, Lamek
Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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AKTIVITAS ANTIOKSIDAN SENYAWA FLAVONOIDA DARI DAUN BENALU KAKAO (Dendrophthoe pentandra (L.) Miq.) Sembiring, Helmina Br.; Lenny, Sovia; Marpaung, Lamek
Chimica et Natura Acta Vol 4, No 3 (2016)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.266 KB) | DOI: 10.24198/cna.v4.n3.10920

Abstract

Penelitian ini bertujuan untuk mendeteksi senyawa fitokimia dan menguji aktivitas antioksidan ekstrak metanol, ekstrak  etil asetat, total flavonoida dan flavonoid aglikona  daun benalu kakao (Dendrophthoe pentandra (L.) Miq.). Serbuk kering daun D. pentandra (L.) Miq. (10 kg) dimaserasi dengan menggunakan metanol, ekstrak metanol diekstraksi kembali dengan etil asetat, ekstrak etil asetat dipartisi dengan n-heksana. Residu yang tersisa (total flavonoida) dihidrolisis dengan HCl 2 N sehingga diperoleh flavonoid aglikona.  Diperoleh ekstrak metanol sebanyak  830 g (8,3%), ekstrak etil asetat 108 g (1,08%), total flavonoida 45 g (0,06%) dan flavonoid aglikona 6,2 g (0,06%) dan semua ekstrak  berwarna hitam. Berdasarkan hasil skrining fitokimia yang dilakukan ekstrak metanol D. pentandra (L.) Miq.  mengandung senyawa flavonoida, tanin, terpenoid, saponin dan tidak terdeteksi adanya alkaloid. Ekstrak etil asetat mengandung senyawa flavonoida, terpenoid dan saponin dan tidak terdeteksi adanya tanin dan alkaloid. Total flavonoida hanya mengandung flavonoida dan tidak terdeteksi adanya saponin, tanin, alkaloid dan terpenoid. Ekstrak metanol, etil asetat, total flavonoida dan flavonoid aglikona daun D. pentandra (L.) Miq. memiliki aktivitas antioksidan dengan nilai IC50  berturut-turut sebesar 30,31; 36,23; 24,07 dan 18,22 mg/mL. Sedangkan asam askorbat sebagai kontrol menunjukkan nilai IC50  12,08  mg/mL. Senyawa flavonoida dari daun benalu kakao (D. pentandra (L.) Miq.) dapat berfungsi sebagai antioksidan namun aktivitasnya lebih rendah dari pada asam askorbat.
AKTIVITAS SITOTOKSIK SENYAWA GOLONGAN FENOLIK DARI EKSTRAK DAUN SIRIH (Piper betle L.) Malik, Abdul; Marpaung, Lamek; Simanjuntak, Partomuan; Nasution, Pandapotan
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 7, No 2 (2017): Vol.7, No.2, Desember 2017
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.997 KB)

Abstract

Senyawa golongan fenolik adalah senyawa aktif dari tanaman sirih yang berpotensi digunakan sebagai bahan obat alami. Penelitian ini bertujuan untuk menguji aktivitas sitotoksik senyawa golongan fenolik dari daun sirih (Piper betle  L.). Aktivitas sitotoksik diuji dengan mengukur nilai LC50 menggunakan metode Brine Shrimp Lethality Test (BSLT). Ekstraksi dilakukan dengan cara maserasi dengan pelarut metanol 96%. Data yang diperoleh dianalisis dengan Probit Analysis. Hasil penelitian menunjukan bahwa senyawa golongan fenolik dari ekstrak metanol daun sirih memiliki aktifitas sitotoksik yang dengan nilai LC50 = 3,92 ppm dengan standart error 0,42 ppm. Kata kunci: Senyawa fenolik, sirih, Piper betle, sitotoksik
Isolation of Flavonoid Compound from Kareumbi Fruit (Homalanthus populneus (Geiseler) Pax) Marpaung, Lamek; Simbolon, Ronal
Journal of Chemical Natural Resources Vol. 6 No. 2 (2024): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v6i2.18120

Abstract

Flavonoid compounds were isolated from Kareumbi fruit (Homalanthus populneus) using a sequential extraction and purification process. Methanol-concentrated extract was partitioned with ethyl acetate and n-hexane. The concentrated methanol extract was separated by column chromatography using a gradient elution of chloroform and methanol, varying the ratio 90:10; 80:20; 70:30; and 60:40 (v/v). Subsequent column chromatography yielded a yellowish-orange paste (19.6 mg, Rf = 0.73) from the chloroform:methanol (70:30) fraction. UV-visible, FT-IR, and 1H-NMR spectroscopic analysis confirmed that the isolated compounds belonged to the flavonol class of flavonoids.
Isolation of Flavonoid Compounds Derived from Buni Leaves (Antidesma bunius (L) Spreng.) Barus, Tonel; Manalu, Ria Agnes Adelina; Marpaung, Lamek
Journal of Chemical Natural Resources Vol. 6 No. 2 (2024): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v6i2.18379

Abstract

The flavonoid compounds from the leaves of buni (A. bunius (L) Spreng.) have been isolated through a process of maceration utilizing methanol as the solvent. The concentrated methanol extract was combined with ethyl acetate. The concentrated ethyl acetate extract was subsequently dissolved in methanol and subjected to partition extraction using n-hexane. The concentrated methanol extract was acidified with 6% HCl, followed by partition extraction using chloroform. The concentrated chloroform extract was isolated using column chromatography, employing an eluent mixture of n-hexane and ethyl acetate in the following ratios: 90:10, 80:20, 70:30, and 60:40 (v/v). The compounds underwent purification via TLC preparative, resulting in a tawny paste with a mass of 30 mg and an Rf value of 0.31. The compound underwent additional identification through the application of Ultraviolet Visible (UV-Vis) spectroscopy, Fourier Transform-Infrared Spectroscopy (FT-IR), and Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), leading to the conclusion that the flavonoid in question is isoflavone.
Isolation of Flavonoids Compounds from Kesumba Keling Leaves (Bixa orellana L.) Lenny, Sovia; Sihombing, Debynati; Marpaung, Lamek
Journal of Chemical Natural Resources Vol. 4 No. 2 (2022): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v4i2.11966

Abstract

Isolation of flavonoid compounds from leaves of Kesumba Keling (Bixa orellana L.). Extraction has been done with maceration by methanol solvent. The concentrated extract of methanol was added with ethyl acetate. The concentrated ethyl acetate extract was then dissolved with methanol and partitioned with n-hexane. The concentrated methanol extract was acidified by 6% HCl, then partition extracted with chloroform. The concentrated chloroform extract was separated by column chromatography with eluent n-hexane: ethyl acetate 90:10; 80:20; 70:30; 60:40; 50:50 (v/ v). The compounds were purified with TLC preparative yielding yellow gum weighing 29.7 mg with Rf=0.51. The compound was further identified by Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infra Red Spectroscopy (FT-IR), and Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR). Spectroscopic data show that the compound was isoflavone.
Analysis of Fatty Acid Composition Using GC-MS Method and Antibacterial Activity Test of n-Hexane Extract from Petai Seeds (Parkia speciosa Hassk.) Marpaung, Lamek; Sinaga, Rio Maretanto
Journal of Chemical Natural Resources Vol. 4 No. 2 (2022): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v4i2.11973

Abstract

Analysis of fatty acid composition using GC-MS method and antibacterial activity test of n-hexane extract from Petai seed (Parkia speciosa Hassk.) has been performed. Fatty acid was extracted from petai seed by maceration method using n-hexane solvent to produce n-hexane extract converted into fatty acid methyl ester through a methanolysis reaction. Then fatty acid composition was analyzed using the GC-MS method. The result of GC-MS analysis showed that the fatty acid composition found in 760 gr petai seeds which produced 11.16 g of fatty acids, was 1.0088 gr palmitic acid methyl ester (9.04%), 1.2700 gr linoleic acid methyl ester (11.38 %), 7.3556 gr oleic acid methyl ester (65.91%) and 1.4887 gr stearic acid methyl ester (13.34%). The antibacterial activity test showed that all concentrations of n-hexane extracts of 50 mg/ml, 150 mg/ml, and 250 mg/ml tested were able to inhibit the growth of E. coli and S. aureus bacteria in n-hexane extract, wherein the inhibited zone by the n-hexane extract is included in the weak category (≤14 mm) except at the concentration of 250 mg/ml in the S. aureus test bacteria belonging to the medium type (15-19 mm).
Isolation of Flavonoids Compounds from Akalifa (Acalypha wilkesiana Muell. Arc.) Plant Leaves Simangunsong, Siska NA; Lenny, Sovia; Marpaung, Lamek
Journal of Chemical Natural Resources Vol. 5 No. 1 (2023): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v5i1.11990

Abstract

Flavonoid compounds from the leaves of Akalifa (Acalypha wilkesiana Muell. Arc.) have been extracted with maceration by methanol solvent, then added with ethyl acetate and partition extracted with n-hexane. 6% HCl acidifies the concentrated methanol extract, then partition extracted with chloroform. The concentrated chloroform extract was separated by column chromatography with silica gel 40 as the stationary phase and n-hexane: ethyl acetate 90:10; 80:20; 70:30; 60:40; 50:50 (v/v) as the mobile phase. The compounds were purified with TLC preparative yielding tawny paste weighing 4 mg with Rf= 0.42 with eluent n-hexane: ethyl acetate 60:40 (v/v). The identified analysis by using Ultraviolet-Visible (UV-Vis) spectroscopy, Fourier Transform Infra Red Spectroscopy (FT-IR), and Proton Nuclear Magnetic Resonance (1H-NMR) Spectroscopy was estimated as a flavonoid is an isoflavone.
Analysis of Fatty Acid Composition Using GC-MS and Antibacterial Activity Test of n-Hexane Extract from Jengkol Seeds (Pithecellobium lobatum Benth.) Marpaung, Lamek; Siahaan, Surya Graha P.
Journal of Chemical Natural Resources Vol. 5 No. 1 (2023): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v5i1.11991

Abstract

Analysis of fatty acid composition using GC-MS and antibacterial activity test of n-hexane extract from Jengkol seeds (Pithecellobium lobatum Benth) was performed with the maceration method to obtain n-hexane extract of Jengkol seeds. The n-hexane extract of Jengkol seeds was first esterified using methanol (1.7 ml): 98% H2SO4 (0.3 ml): Chloroform (2 ml), then analyzed its fatty acid composition by GC-MS method. The results of fatty acid composition analysis found in Jengkol seeds are saturated fatty acids, namely: Margaric Acid (1.10%), Palmitate Acid (2.64%), stearic acid (12.01%), and unsaturated fatty acids, namely: Linolelaidate acid (10.02%) %), and oleic acid (74.23%). The antibacterial activity test against Escherichia coli and Staphylococcus aureus using n-hexane extract of Jengkol seed was done by agar diffusion method. The concentration variations were 50 mg/ml, 150 mg/ml, and 250 mg/ml. From the results of this test, n-hexane Jengkol seeds have an antibacterial activity that can inhibit bacterial growth but is weak (≤14 mm).
Isolation and Identification of Flavonoid Compounds and Antibacterial Activity Test of the Canyere Badak Plant (Bridelia glauca Blume) (Phyllanthaceae) Marpaung, Lamek; Toruan, Ade Theresia Ulina Lumban
Journal of Chemical Natural Resources Vol. 5 No. 2 (2023): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v5i2.13816

Abstract

Flavonoid compounds from the stem bark of the Canyere Badak plant (Bridelia glauca Blume) have been isolated. 2500 g of Canyere Badak plant stem bark was macerated by methanol, and the methanol extract dissolved in distilled water. The distilled water solution was extracted by partitioning with ethyl acetate repeatedly until negative to 5% FeCl3. The ethyl acetate extract was dissolved in methanol and extracted by partitioning with n-hexane until the n-hexane layer was transparent. The methanol extract was analyzed by thin layer chromatography and separated by column chromatography with a stationary phase of silica gel and a mobile phase of chloroform: methanol (90:10; 80:20; 70:30; 60:40) v/v. Fractions 148-174 were purified by preparative thin layer chromatography using benzene: acetone (80:20) v/v eluent to produce a brownish yellow paste of 10 mg at Rf value of 0,66 using chloroform: methanol (80:20) v/v  as eluent. Based on the analysis of the UV-visible spectrophotometer, it had a wavelength (λ max) of 275 nm. The FT-IR spectrum shows the presence of OH, C=C aromatic, C=O ketones, C-H, C-O-C and C-O groups. The proton Nucleus Magnetic Resonance Spectrum (1H-NMR) indicates the presence of H-2’ & H-6’ protons, H-3’ & H-5’ protons and methoxy protons. Based on the data analysis and interpretation, the isolated compound was a flavonoid of the flavanones group. The antibacterial activity of total flavonoids was determined by agar disk diffusion against Staphylococcus aureus and Escherichia coli bacteria. The results showed that total flavonoids strongly inhibited the growth of Staphylococcus aureus and Escherichia coli bacteria with MIC (Minimum Inhibitory Concentration) value at a concentration of 100 mg/mL obtained a clear zone of 9.5 mm against Staphylococcus aureus bacteria and at a concentration of 100 mg/mL obtained a clear zone of 12.15 mm against Escherichia coli bacteria.
Co-Authors A.A. Ketut Agung Cahyawan W Manalu, Ria Agnes Adelina Mastura, Mastura Nasution, Pandapotan Partomuan Simanjuntak Sembiring, Helmina Br. Siahaan, Surya Graha P. Sihombing, Debynati Simangunsong, Siska NA Simbolon, Ronal Sinaga, Rio Maretanto Sovia Lenny Tonel Barus, Tonel Toruan, Ade Theresia Ulina Lumban