POTENSI PENGINDUKSI APOPTOSIS SENYAWA NIKOTINIL DIBUTIL GLUTAMAT ESTER TERHADAP SEL KANKER T47D

Chandra Risdian (LIPI)
Tjandrawati Tjandrawati (LIPI)
Muhamad Hanafi (LIPI)
Yulia Anita (LIPI)
AMJ Putra (LIPI)

Widyariset
Vol 12, No 1 (2009): Widyariset

Nicotinil Dibuthyl Glutamate Ester (NDBGE) is a synthetic compound that is an analog of antibiotic UK3A from mycelium of Streptomyces sp.517-02. This compound was estimated to have anticancer activity similar to UK-3A. The aim of this research was to know the NDBGEs apoptotic inducing potency to human breast cancer T47D in vitro. DMEM + 1% PS + 10% FBS was added to human breast cancer T47D cells until cell concentration reached 1-5x/ 05eells/ml. NDBGE then was diluted by DMSO until 100ppm concentration. Propidium iodide with 50pg/m1 concentration was added to the T47D cells. The analysis was conducted by flow cytometry method. The result was that the apoptosis percentage of the cells was 12,35%.