Bioscientist : Jurnal Ilmiah Biologi
Vol 11, No 1 (2023): June

Efek Perbedaan Temperatur terhadap Aktivitas Tripsin-Like dan Amilase Digesti Ikan Lunjar (Rasbora lateristriata Blkr.)

Fadhna Alunka Majid (Program Studi Biologi, Fakultas Biologi, Universitas Jenderal Soedirman)
Untung Susilo (Program Studi Biologi, Fakultas Biologi, Universitas Jenderal Soedirman)
Eko Setio Wibowo (Program Studi Biologi, Fakultas Biologi, Universitas Jenderal Soedirman)
Yulia Sistina (Program Studi Biologi, Fakultas Biologi, Universitas Jenderal Soedirman)



Article Info

Publish Date
30 Jun 2023

Abstract

Previous studies related to digestive enzyme activity have been carried out, but there is no information related to the effect of temperature on the trypsin-like activity and digestive amylase in Rsbora lateristriata. Therefore, the purpose of this study was to determine the effect of temperature differences on the activity of trypsin-like and amylase digestion of Rasbora lateristriata fish. The temperature treatments applied were 20, 30, 40, 50, 60, and 70ºC and each treatment was repeated five times. A total of 125 Rasbora fish with a body weight of 0.81 ± 0.09 grams were used in this study. Trypsin-like and amylase activities were measured using a spectrophotometer. The results showed that there were significant differences (p < 0.05) in the trypsin-like and amylase activities at different temperatures. The trypsin-like activity was not significantly different (p < 0.05) between 20-50ºC, but the activity was lower at 60-70ºC. Amylase activity at 30°C and 40°C was not significantly different (p < 0.05), but significantly different from 20, 50, 60, and 70°C. In conclusion, trypsin-like decreased activity at 60°C and amylase at 50°C.

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Journal Info

Abbrev

bioscientist

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

Description

Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing ...