OPTIMIZATION OF ENZYME-MICROWAVE ASSISTED EXTRACTION, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF POLYSACCHARIDE FROM Ganoderma lucidum

Lira Windriawati Listriyani (a:1:{s:5:"en_US"s:4:"BRIN"})
Sumi Hudiyono (Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Indonesia, Kampus UI Depok, Depok, 16424, Indonesia)
. Rudiyono (Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency, Indonesia)
Siti Zulaeha (Research Center for Genetic Engineering, National Research and Innovation Agency, Indonesia)
Ahmad Wibisana (Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency, Indonesia)

Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Vol. 10 No. 1 (2023): Juni 2023

Enzyme-Microwave Assisted Extraction (EMAE) is a new process for extracting Ganoderma lucidum polysaccharides (GLPs). Cellic® CTec2 was chosen as an enzyme that assists in microwave extraction. The four variables involved in this study were enzyme concentration (%), enzymatic reaction time (minutes), solvent-to-solid ratio (mL/g), and microwave extraction time (minutes). This study showed that the enzyme concentration and solvent-to-solid ratio had a significant effect on the response in the range studied. Yield extraction of polysaccharides from experiments conducted at optimum conditions showed good agreement with the predictions from the model. The EMAE method showed a higher polysaccharide extraction yield than hot water extraction (HWE) method. GLPs from EMAE method had antioxidant activity of 79.47 ± 0.71% (DPPH) and 0.884 ± 0.013 mM Fe2+/L (FRAP), where these values were higher than those of the HWE method.