Synthesis of Human Antibodies Against HBsAg in Newly Established Chinese Hamster Lung (CHL-YN) Cell Line

Meutia Diva Hakim (Institut Teknologi Bandung, School of Life Sciences and Technology, Department of Biotechnology, Bandung, 40132, Indonesia)
Noriko Yamano- Adachi (Osaka University, Graduate School of Engineering, Department of Biotechnology, and OTRI, Industrial Biotechnology Initiative Division, Osaka, 565-0871, Japan)
Takeshi Omasa (Osaka University, Graduate School of Engineering, Department of Biotechnology, and OTRI, Industrial Biotechnology Initiative Division, Osaka, 565-0871, Japan)
Marselina Irasonia Tan (Institut Teknologi Bandung, School of Life Sciences and Technology, Department of Biotechnology, Bandung, 40132, Indonesia)
Lia Dewi Juliawaty (Institut Teknologi Bandung, Faculty of Mathematics and Natural Sciences, Department of Chemistry, Bandung, 40132, Indonesia)
Ernawati Arifin Giri-Rachman (Institut Teknologi Bandung, School of Life Sciences and Technology, Department of Microbiology, Bandung, 40132, Indonesia)

Journal of Mathematical and Fundamental Sciences
Vol. 54 No. 3 (2023)

Hepatitis B immunoglobulin (HBIG) is an effective treatment for hepatitis B, including postexposure prophylaxis of HBV infection, prevention of HBV reinfection in liver transplant patients, and reducing sexual transmission. This study investigated the synthesis of human IgG antibodies that specifically target HBsAg subtype adr in CHL-YN cells, a newly established cell line that grows faster than CHO-K1. To achieve the synthesis of human IgG antibodies, a plasmid vector encoding DNA sequences for human IgG antibodies against HBsAg was constructed and then transiently transfected into CHL-YN cells. The expression and antigen-binding capacity of the recombinant human IgG antibodies were analyzed using western blot and ELISA. The results showed successful expression and secretion of human IgG antibodies that recognize HBsAg subtype adr in CHL-YN cells. The ELISA test confirmed the specificity of the human IgG antibodies towards HBsAg subtype adr. Thus, this study concluded that human IgG antibodies that target HBsAg subtype adr were transiently expressed in CHL-YN cells.