agriTECH
Vol 43, No 3 (2023)

Preliminary Study of Sonication Time for Extracting Natural Dye and Antioxidant from Sargassum duplicatum

Ummi Roudhoh (Fisheries Product Technology Study Program, Faculty of Fisheries and Marine Sciences, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang 50275)
Romadhon Romadhon (Fisheries Product Technology Study Program, Faculty of Fisheries and Marine Sciences, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang 50275)
Ahmad Suhaeli Fahmi (Fisheries Product Technology Study Program, Faculty of Fisheries and Marine Sciences, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang 50275)



Article Info

Publish Date
30 Sep 2023

Abstract

Sargassum duplicatum is a brown seaweed containing various chemical compounds with potential applications as a natural dye and antioxidant. These compounds include alginate, protein, vitamin C, tannins, iodine, phenols, and pigments such as chlorophyll a, chlorophyll c, fucoxanthin, xanthophyll, and carotene. Therefore, this study aimed to determine the effect of varying extraction times on the color and antioxidant quality of Sargassum duplicatum extract. One of the methods used to extract Sargassum duplicatum is ultrasonication, which involves using ultrasonic waves to break down the cell walls of the material and facilitate efficient compound extraction. The extraction was carried out at 15 (A), 30 (B), and 45 minutes (C), with three repetitions for each treatment. The assessed parameters included yield calculation, total carotenoids, antioxidant activity, and color intensity. Parametric data were analyzed using ANOVA and Tukey HSD follow-up tests. The extraction process used an 80% ethanol solvent at 40 °C and a frequency of 42 kHz. This study employed an experimental laboratory approach using a completely randomized design (CRD) with different extraction times. The results showed significant differences (p<5%) in yield value, total carotenoids, antioxidant activity, and color intensity of Sargassum duplicatum extract at different extraction times. The best results were obtained at 45 minutes, yielding 86.66%, with 9.62% total carotenoids, an IC50 value of 1241.02 ppm, and lightness (L*), redness (a*), and yellowness (b*) values of 4.44, -3.46, and 37.90, respectively.

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Journal Info

Abbrev

agritech

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Agritech with registered number ISSN 0216-0455 (print) and ISSN 2527-3825 (online) is a scientific journal that publishes the results of research in the field of food and agricultural product technology, agricultural and bio-system engineering, and agroindustrial technology. This journal is ...