JURNAL AGROTEKNOLOGI
Vol 5, No 2 (2015): Februari 2015

OPTIMASI NAA DAN BAP TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TUNAS MIKRO TANAMAN KANTONG SEMAR (Nepenthes mirabilis) SECARA IN VITRO

ROSMAINA ROSMAINA (Fakultas Pertanian dan Peternakan UIN Sultan Syarif Kasim Riau)
DINNI ARYANI (Fakultas Pertanian dan Peternakan UIN Sultan Syarif Kasim Riau)



Article Info

Publish Date
01 Feb 2015

Abstract

Conventional propagation of Nepenthes was difficult to do. To overcome the problems were required alternative method such as in vitro propagation. The objective of this research was to obtain the best treatment of BAP + NAA on shoot multiplication of Nepenthes through in vitro culture. The research design used Randomized Completely Design consist of seven treatments, e.g. 1) ½ MS0 (control); 2) ½ MS + 1 ppm BAP + 0.5 ppm NAA; 3) ½ MS + 1 ppm BAP + 1 ppm NAA; 4) ½ MS + 1.5 ppm BAP + 0.5 ppm NAA; 5) ½ MS + 1.5 ppm BAP + 1 ppm NAA; 6) ½ MS + 2 ppm BAP + 0.5 ppm NAA dan 7) ½ MS + 2 ppm BAP + 1 ppm NAA. The parameter observed were number of shoot, number of nodul, number of leafs, number of pitcher and number of root. The result of this research showed that treatment of ½ MS + 1 ppm BAP + 1 ppm NAA is the best treatment compared to others. At induction stage, this treatment can produce the number of shoot, number of nodul, and number of root were 1.6 shoots/explant, 10.8 nodul/explant and 3.6 root/explant, respectively. At subculture, this treatment can produce the number of shoot, number of leafs, and number of pitcher were 5.8 shoots/explant, 12.4 leafs/explant and 5.2 pitcher/explant, respectively.

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Journal Info

Abbrev

AGROTEKNOLOGI

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal Agroteknologi diterbitkan Fakultas Pertanian dan Peternakan Universitas Islam Negeri Sultan Syarif Kasim Riau. Jurnal ini mengakomodir artikel/karya ilmiah meliputi agronomi, tanah, hama dan penyakit, teknologi benih, pemuliaan, teknologi hasil pertanian, agroindustri, dan sosial ekonomi ...