Rosmaina Rosmaina
Department Of Agrotechnology, Faculty Of Agriculture And Animal Husbandary, Universitas Islam Negeri Sultan Syarif Kasim Riau, Pekanbaru

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INDUKSI KALUS PASAK BUMI (Eurycoma longifolia Jack) MELALUI EKSPLAN DAUN DAN PETIOL ULFIATUN, ULFIATUN; MAISUPRATINA, MAISUPRATINA; ROSMAINA, ROSMAINA; ZULFAHMI, ZULFAHMI; SUTEJO, PROBO
JURNAL AGROTEKNOLOGI Vol 6, No 1 (2015): Agustus 2015
Publisher : JURNAL AGROTEKNOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One of the problem of Eurycoma longifolia Jack propagation was low germination percentage due to recalcitrant seeds and slow growth of seedling from cutting propagation. To overcome this problem is required propagation of Eurycoma longifolia via in vitro culture. The objective of this research was to know the effect of Auxin (2,4-D and NAA) and Cytokines (BAP and Kinetin)  on Eurycoma longifolia callus induction via leaf and petiole explants. In this study, we used plant growth regulator of 2,4 D, NAA, BAP and Kinetin in several levels.  The observed variables were appearing callus time, callus color and callus texture. The results of this study showed that MS medium supplemented with 1 ppm NAA+ 1 ppm BAP was able to induce callus formation in leaf explant for 6 months after culture. While MS medium supplemented with 1 ppm 2,4-D, 1 ppm BAP, combination of 2,4-D and Kinetin and combination of 2,4-D and BAP can induce callus formation from petiole. All the callus formation has yellow color and yellow brown color. The petiole explant that is grown in MS medium supplemented with 1 ppm BAP induced of callus in short time (18 days after culture).
NEW RECORD OF EURYCOMA APICULATA A.W. BENN (SIMAROUBACEAE) FROM FOREST RESERVE OF KENEGERIAN RUMBIO, RIAU, INDONESIA Zulfahmi, Zulfahmi; Aryanti, Ervina; Rosmaina, Rosmaina
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3683

Abstract

During extensive field surveys in the 2015 year, Eurycoma apiculata A.W. Benn has been found for the first time in the forest reserve of Kenegerian Rumbio, Kampar District of Riau province. This finding is a new record of the medicinal plant in Indonesia. The taxonomic description and photographs of the species are given in this article.  
MORFOLOGI PERMUKAAN DAUN TANAMAN TERUNG (Solanum melongena L.) SEBAGAI RESPONS TERHADAP CEKAMAN KEKERINGAN Ilahi, Rizky Nanda Kurnia; Isda, Mayta Novaliza; Rosmaina, Rosmaina
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.312 KB) | DOI: 10.15408/kauniyah.v11i1.5662

Abstract

Abstrak Terung (Solanum melongena L.) tergolong tanaman yang sensitif terhadap kekeringan selama tahap pertumbuhan dan perkembangannya. Karakteristik stomata dan trikoma merupakan kriteria yang dapat digunakan untuk mengidentifikasi tanaman yang toleran terhadap kekeringan. Penelitian bertujuan menguji respons karakteristik anatomi daun berupa trikoma dan stomata tanaman terung terhadap cekaman kekeringan melalui empat taraf interval penyiraman. Penelitian menggunakan Rancangan Acak Lengkap melalui empat taraf interval penyiraman, yakni 3, 6, 9, dan 12 hari.  Pengaruh cekaman kekeringan terhadap morfologi permukaan daun pada terung menyebabkan terjadinya peningkatan kerapatan trikoma tiga kali lebih banyak dibandingkan dengan kontrol, penurunan ukuran lebar trikoma mencapai 59,02%, penurunan ukuran lebar stomata mencapai 78,34%, dan penurunan ukuran lebar porus stomata mencapai 80,80%. Hasil penelitian menunjukkan bahwa peningkatan jumlah trikoma dengan ukuran trikoma yang semakin kecil diduga sebagai bentuk proteksi tanaman terhadap kerusakan jaringan dan mekanisme adaptasi tanaman untuk dapat memenuhi jumlah asimilasi CO2 perluas daun yang dibutuhkan untuk fotosintesis pada kondisi kekeringan. Sensitifitas tanaman terhadap kondisi kekeringan berupa mekanisme adaptasi dengan cara memperkecil ukuran stomata dan bukaan lebar porus, sehingga laju fotosintesis tetap terjaga pada kondisi kekeringandan mekanisme tanaman menjaga efisiensi penggunaan air dengan cara mengurangi ukuran stomata dan memperkecil bukaan porus stomata.    Abstract Eggplant (Solanum melongena L.) belongs to a group of plants that are sensitive to drought (water stress) during their growth and development stages. Characteristics of stomata and trichomes are criteria that can be used to identify drought-tolerant plants. This study aims to determine the response of leaf anatomical characteristics of the eggplant as well as trichome and stomata to drought stress through four levels of watering interval. The research using completely randomized design with watering intervals of 3, 6, 9, and 12 days. The effect of drought stress on leaf surface morphology of the eggplant resulted in three times greater trichomes density than control, decreased trichomes width by 59.02%, stomata width by 73.84%, and size of stomata porch width by 80.80%.. The result was showing that increasing number of trichome with smaller trichome size was thought to be a form of crop protection against tissue damage and plant adaptation mechanism in order to meet the amount of CO2 leaf expansion assimilation required for photosynthesis in drought stress condition. The sensitivity of plants to drought stress conditions is the mechanism of adaptation by reducing the size of stomata and wide porous opening, so that the rate of photosynthesis has been maintaining in the dry conditions and the mechanism of the plant maintain the efficiency of water use by reducing the size of stomata and minimizing stomata porous opening.   Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 5667
Pengaruh Praperlakuan Dingin Antera terhadap Pembentukan Kalus Cabai Merah Keriting (Capsicum annuum L.) Pretreatment on the Callus Formation of Curly Red Chilli Pepper (Capsicum annuum L.) M Solin, Nida Wafiqah Nabila; Adriani, Dian; Zulfahmi, Zulfahmi; Irfan, Mokhamad; Rosmaina, Rosmaina
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 4, No 2 (2020): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.103 KB) | DOI: 10.31289/agr.v4i2.3533

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The production of the double haploid plant in vitro through anther culture technique is a plant breeding technique used to obtain pure strain rapidly. A variety of pretreatment has been reported to induce callus and regenerate planlets efficiently. This study aims at describing the influence of cold anther pretreatment towards the callus formation of curly red chili pepper (Capsicum annuum L.). This research was conducted in the laboratory of Genetics and Breeding, Faculty of Agriculture and Animal Science, Universtas Islam Negeri Sultan Syarif Kasim Riau. The explants used are anther of local genotype of curly red chili pepper. The anthers are stored at low temperatures (4 °c) with different time intervals of 0, 24, 48 and 72 hours. The results showed that the percentage of highest callus formation was obtained at 24 and 72 hours length storage, ie 50%. Cold pretreatment of 72 hours anther storage results in a faster callus with a percentage of the highest yellowish white callus color of 17.65% and a compact structure. The cold pretreatment with 72 hours anther storage is the most optimal acceleration in the development stage of anther culture and induces te formation of curly red chili pepper (Capsicum annuum L.) local genotypes.
The Application Of PCR (Polymerase Chain Reaction) Using Specific Primer To Detect Chillies Drought Tolerant Ria Oktavianti
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol 1 No 2 (2019): July. Jurnal Agronomi Tanaman Tropika (JUATIKA)
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.24 KB) | DOI: 10.36378/juatika.v1i2.176

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The purpose of this study was to find tolerant genotypes. This research was conducted on 16 chilli genotypes from UIN Suska Riau Laboratory of Breeding and Genetics. Using four specific primers wich were TIL, DREB2A, P5CS, and Primary Pair 2 (BLAS). Results of the study showed amplifikasi of chilli DNA could be obtained using TIL and Pair 2. Primer however th corsponding result were obtain only by using TI to be drought tolerant, while 5 genotypes were found drought intolerant. The drought tolerant genotypes were (RFC-005), (RFC-006), (G-UIN- 016), (G-UIN-015), (G-UIN-065), (UIN-097), (UIN-035), (UIN-100), (UIN-099), (UIN-037) and (UIN-096). While 5 intolerant genotypes were (G-UIN-019), (G-UIN-008), (G-UIN-038), (RFC-013) and (UIN-036).
Genetic Diversity of Eurycoma longifolia Jack Based on Random Amplified Polymorphic DNA Marker Rosmaina Rosmaina; Zulfahmi Zulfahmi
Jurnal Manajemen Hutan Tropika Vol. 19 No. 2 (2013)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (594.192 KB) | DOI: 10.7226/jtfm.19.2.138

Abstract

Eurycoma longifolia Jack is one of the extensively exploited medicinal plants in Indonesia. The objectives of this study were to obtain information on genetic diversity and population genetic structure of E. longifolia to formulate effective conservation plan. RAPD marker was used to assess the genetic diversity of E. longifolia collected from 5 natural populations in Riau Province. A total of 25 plants were analyzed using 5 RAPD primers, which amplified produced 44 scored DNA bands. The mean observed number of alleles per locus (No), number of effective alleles (Ne), and percentage of polymorphic loci (PPL) of E. longifolia were 1.57, 1.34, and 56.80%, respectively. The degree of differentiation among populations of E. longifolia was 0.31 (Ht = 0.29; Hs = 0.20).  The mean value of estimated gene flow among populations of E. longifolia was 1.11 individual per generation. The UPGMA dendogram formed 2 significant clusters. The first cluster consisted of Pelalawan and Kampar populations, while the second cluster was formed from Kuansing, Rohul, and Rohil population. The genetic diversity information in this study is very important to perform efficient conservation and effective future management of its genetic resources.  
Korelasi dan Analisis Lintas Beberapa Karakter Tanaman Cabai (Capsicum annuum L.) pada Kondisi Normal dan Tercekam Kekeringan (Correlations and Path Analysis of Some Characters in Chili Pepper (Capsicum annuum L.) Under Normal and Drought Stress) nFN - Rosmaina; nFN Sobir; nFN Parjanto; Ahmad Yunus
Jurnal Hortikultura Vol 29, No 2 (2019): Desember 2019
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v29n2.2019.p147-158

Abstract

Cekaman air merupakan salah satu faktor pembatas pertumbuhan dan produksi tanaman termasuk cabai (Capsicum annuum L.). Produktivitas tanaman merupakan karakter yang kompleks sehingga hubungan antarkarakter perlu diketahui untuk mendapatkan kriteria seleksi yang tepat untuk perbaikan tanaman. Penelitian ini bertujuan mengetahui kriteria seleksi terhadap produksi tanaman cabai toleran kekeringan pada fase pembungaan. Penelitian disusun menggunakan Rancangan Acak Lengkap dengan tiga taraf cekaman air, yaitu 100% kapasitas lapang (kontrol), 50% kapasitas lapang (medium stress), dan 25% kapasitas lapang (extreme stress). Hasil analisis korelasi menunjukkan bahwa berbagai karakter yang diamati memperlihatkan perbedaan asosiasi dengan level cekaman kekeringan. Karakter panjang akar tidak dapat digunakan sebagai kriteria seleksi tanaman cabai yang toleran terhadap kekeringan karena panjang akar tidak berkorelasi secara signifikan dengan bobot buah per tanaman pada 50% dan 25% kapasitas lapang. Berdasarkan korelasi dan analisis lintas pada kondisi normal (100% kapasitas lapang) dan kondisi tercekam (50% kapasitas lapang), karakter jumlah buah dan persentase fruit set berkorelasi positif dan berpengaruh langsung terhadap bobot buah per tanaman sehingga dapat digunakan sebagai kriteria seleksi untuk perbaikan tanaman cabai yang toleran kekeringan.KeywordsCekaman air; Cekaman kekeringan; Kriteria seleksi; Cabai; Capsicum annuum LAbstractWater stress is one of limiting factors for plant growth and production, including chili (Capsicum annuum L.). Plant productivity is a complex character so the relationship between characters needs to be known to get the right selection criteria for crop improvement. This study aimed to determine the selection criteria for the production of drought-tolerant chili pepper at the flowering phase. The research arranged under completely randomized design with three levels of water stress, namely 100% water field capacity (control), 50% water field capacity (medium stress) and 25% water field capacity (extreme stress). The results of the correlation analysis showed that the various characters observed exhibited differences associations with drought stress levels. Root length cannot be used for selection criterium of drought tolerant on chili because it was no positive significantly correlated to fruit weight per plant at 50% and 25% water field capacity. Based on correlation and path analysis in normal and drought conditions, the number of fruits and percentage of fruit set can be used as selection criteria for genetic improvement of drought-tolerant on chili plants because these characters were significantly positively correlated and had a direct effect on fruit weight of the plant.
KEPADATAN DAN POLA PENYEBARAN PASAK BUMI (Eurycoma longifolia Jack) DI ZONA ALAMAN KUYANG, HUTAN LARANGAN ADAT KENEGARIAN RUMBIO ZULFAHMI ZULFAHMI; NELAWATI NELAWATI; ROSMAINA ROSMAINA
Jurnal Agroteknologi Vol 6, No 1 (2015): Agustus 2015
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v6i1.1375

Abstract

The objective of this research was to know the density and distribution pattern of E. longifolia in the Alaman Kuyang zone of the forest reserve of Kenegerian Rumbio. The research used purposive sampling method. The observation plot is made in square plot  which plot size was 20 x50 m.  Results of this study showed that density  of E. longifolia in Alaman Kuyang zone of forest reserve of Kenegarian Rumbio was 130 individual ha-1, which seedling phase is more dominance than  sapling and pole phases. The density of E.longifolia was low due to increasing the harvesting of this species from forest reserve of Kenegarian Rumbio, and addition poor of mother trees in area study. Distribution pattern of E. longifolia were clumped with Morisita Index value > 1, this could be explained since seed of E. longifolia dispersed and grew not far from mother trees.
LAJU MULTIPLIKASI TUNAS NENAS (Ananas comosus L. Merr) PADA MEDIA DASAR MURASHIGE AND SKOOG HASIL PERLAKUAN BA DAN NAA SECARA IN VITRO Rosmaina Rosmaina
Jurnal Agroteknologi Vol 1, No 1 (2010): Agustus 2010
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v1i1.49

Abstract

The objectives of this research are to study shoots multiplication rate of Pineapple (Ananas comosus L. Merr) on Murashige and Skoog (MS) basal medium from BA, NAA and its combination treatment by in vitro culture. The experimental design was completely randomize design with two factorials. The first factor is BA consists of 0.00 μM, 4.44 μM, 8.88 μM, 13.32 μM and 17.76 μM, and the second factor is NAA consist of 0.00 μM, 0.50 μM, 1.00 μM and 2.00 μM. The result of this study showed that BA, NAA and its combination could increase the shoot multiplication rate of pineapple on basal medium Murashige and Skoog. BA and NAA combination produce high multiplication rate than BA without NAA or NAA no added of BA. The best treatment in this study was 4.44 mM NAA + 0.5 mM NAA, produce 107.001,20 planlets/year in Murashige and Skoog basal Medium with uniform, normal and healthy planlet.
Keanekaragaman Tanaman Kantong Semar (Nepenthes spp.) di UIN SUSKA Riau Pitcher Plant (Nepenthes spp) Diversity in the UIN SUSKA RIAU Rosmaina Rosmaina; Zulfahmi Zulfahmi; Rizka Rosa
Jurnal Agroteknologi Vol 2, No 2 (2012): Februari 2012
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v2i2.126

Abstract

Forest of the UIN SUSKA Riau was harboring most of the genetic resources, one of them is pitcher plants (Nepenthes spp). Pitcher plants have high economic value because of they were ornamental and medicinal plants. This research aims to determine population density, diversity index, evenness index and dominance index of Nepenthes in the campus forest of UIN SUSKA Riau. This research used method was purposive sampling with square Jalur method. The result of this study found two species of Nepenthes in the campus of UIN SUSKA Riau, namely Nepenthes gracillis and Nepenthes ampularia. The average of population density of Nepenthes gracillis and Nepenthes ampularia were 2000 individuals/ha and 200 individuals/ha, respectively. The Diversity index of Nepenthes in this study was low (H’ <1), while average of evenness index (E) of Nepenthes was 0.352.