Journal of Research in Pharmacy and Pharmaceutical Sciences
Vol 2 No 1 (2023): Vol.2 No.1 JUNE (2023)

ANTIBIOTIC SUSCEPTIBILITY TEST OF PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS WITH DISK DIFFUSION AND DILUTION METHOD

Raffi Joe Bhagaskara (UPN Veteran Jakarta)
Alyaa Shofura Ahmad (UPN Veteran Jakarta)
Syifa Regina Prasetyawan (UPN Veteran Jakarta)
Dewi Amelia (UPN Veteran Jakarta)
Deri Putra Pratama (UPN Veteran Jakarta)
Innayah Marda Hidayyah Tulloh (UPN Veteran Jakarta)
Naadhira Alya Putri (UPN Veteran Jakarta)
Indira Damayanti Putri (UPN Veteran Jakarta)
Alya Layyina Rabbani (UPN Veteran Jakarta)
Fathan Mubiin Ibnu Ismail (UPN Veteran Jakarta)
Jihan Tria Astuti (UPN Veteran Jakarta)
Naufal Brian Atmaja (UPN Veteran Jakarta)



Article Info

Publish Date
25 Jun 2023

Abstract

The susceptibility test is a test used to measure bacteria's sensitivity and vulnerability towards antibiotics. This study was to determine sensitivity of Pseudomonas aeruginosa and Staphylococcus aureus towards amoxicillin, neomycin, and sulfanilamide. In this study, the methods used in susceptibility tests are disk diffusion method and serial dilution. The disk diffusion method is a method with paper disks that already saturated the antibiotics, with one paper disk for each antibiotic, put the disk on the agar media that had been inoculated by the bacteria, then incubated and measured the inhibition zone. The dilution method was done by making a series of antibiotic dilutions in liquid media which added microbes inside. This test is to estimate the smallest concentration or Minimum Inhibitory Concentration (MIC) in the dilution series with no bacterial growth. The diffusion test shows no clear visible zone at all, so Pseudomonas aeruginosa and Staphylococcus aureus are resistant to amoxicillin, neomycin and sulfanilamide. The dilution test shows the clearest sample was on 0,25% concentrate, so amoxicillin's Minimum Inhibitory Concentration towards Staphylococcus aureus is 0,25%.

Copyrights © 2023






Journal Info

Abbrev

JRPPS

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Other

Description

The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of pharmacy. This journal encompasses original article, review article and short communications in the area of pharmacy, including: Pharmacognosy and ...