Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering)
Vol 12, No 4 (2023): December 2023

Application of Pb-Resistant Bacteria to Reduce Pb-Accumulation in Brassica sp. on Pb-Contaminated Soil

Beauty Laras Setia Pertiwi (Postgraduate Student Soil and Water Management Study Program, Faculty of Agriculture, Brawijaya University)
Yulia Nuraini (Department of Soil Science, Faculty of Agriculture, Brawijaya ¬University)
Sugeng Prijono (Department of Soil Science, Faculty of Agriculture, Brawijaya ¬University)
Reni Ustiatik (Department of Soil Science, Faculty of Agriculture, Brawijaya ¬University)



Article Info

Publish Date
02 Dec 2023

Abstract

Brassica sp. is a horticultural crop with high demand for consumption. To meet the demand, farmers apply intensive farming to increase yields and prevent plant pests and diseases that cause yield loss. Agrochemical applications in the form of inorganic fertilizers and pesticides contribute to lead (Pb) contamination in agricultural soils and increase lead (Pb) content in the cultivated plants. Nowadays, using bacteria for remediation (bioremediation) is environmentally friendly and effective in cleaning pollutants by converting organic Pb into inorganic Pb which is less toxic. This study aims to explore Pb-resistant bacteria that can reduce Pb-accumulation on food crops such as Brassica sp. grown in Pb-contaminated soil. This study isolated 15 isolates that survived on nutrient agar containing 1,000 mg/L Pb(NO3)2. The study showed 2 potential Pb-resistant bacteria that reduced Pb accumulation in Brassica sp. up to 30.5%. The bacteria are gram-positive bacilli and non-human pathogens (PT-3 and PT-5). DNA barcode identification results showed the isolates identified as Bacillus altitudinis (PT-3) and Bacillus wiedmannii (PT-5). Aplication of the bacteria increases the shoot length and fresh weight of Brassica sp. Application of the bacteria improves food crops quality by reducing heavy metals accumulation, such as Pb. Thus, the bacteria are potential as biofertilizers for reducing agrochemicals use in intensive agriculture areas and preventing environmental destruction and food contamination. Keywords:   Bioremediation, Brassica sp., Inorganic Fertilizers, Lead-Resistant Bacteria, Pesticides. 

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Journal Info

Abbrev

JTP

Publisher

Subject

Agriculture, Biological Sciences & Forestry Engineering

Description

Jurnal Teknik Pertanian Lampung or Journal of Agricultural Engineering (JTEP-L) is a peer-reviewed open-access journal. The journal invites scientists and engineers throughout the world to exchange and disseminate theoretical and practice-oriented researches in the whole aspect of Agricultural ...