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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrikultura Sains Tanah JTSL (Jurnal Tanah dan Sumberdaya Lahan) Journal of Tropical Biodiversity and Biotechnology Jurnal Penelitian Pendidikan IPA (JPPIPA) SAINS TANAH - Journal of Soil Science and Agroclimatology Dinamisia: Jurnal Pengabdian Kepada Masyarakat Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Spizaetus: Jurnal Biologi dan Pendidikan Biologi Natural Science: Jurnal Penelitian Bidang IPA dan Pendidikan IPA
Ustiatik, Reni
Departemen Ilmu Tanah, Fakultas Pertanian, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Physics Social Sciences Other

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Isolation of Mercury-Resistant Endophytic and Rhizosphere Microorganisms from Grasses in Abandoned Gold Mining Area Reni Ustiatik; Yulia Nuraini; Suharjono; Eko Handayanto
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 49 No. 1 (2021): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (595.264 KB) | DOI: 10.24831/jai.v49i1.32356

Abstract

Terdapat sekitar 900 titik pertambangan emas skala kecil (PESK) di Indonesia yang memperoleh emas melalui teknik amalgamasi dan sianidasi. Teknik amalgamasi menyebabkan pencemaran merkuri (Hg) di tanah. Penelitian ini merupakan penelitian pendahuluan (preliminary study) yang bertujuan untuk mengisolasi mikroorganisme endofit dan rhizosfer resisten Hg dari rumput pionir yang tumbuh di tanah yang tercemar Hg. Mikroorganisme paling berpotensi akan diaplikasikan pada fitoremediasi merkuri di penelitian selanjutnya. Sampel rumput pionir diambil dari lahan pertanian bekas kawasan pertambangan emas dengan teknik amalgamasi di Desa Bonjeruk, Kecamatan Jonggat, Kabupaten Lombok Tengah, Nusa Tenggara Barat. Total mikroorganisme dihitung menggunakan Colony Forming Unit (CFU) atau Standard Plate Count. Koloni mikroorganisme dikarakterisasi berdasarkan ciri morfologi. Mikroorganisme endofit dan rizosfer yang resisten Hg berhasil diisolasi dari rumput pionir (Cynodon dactylon dan Eleusine indica) di lokasi penelitian. Koloni mikroorganisme rizosfer sangat beragam secara morfologi dibandingkan dengan mikroorganisme endofit berdasarkan jumlah mikroorganisme terisolasi, berturut-turut 20 isolat dan 17 isolat. Kepadatan mikroorganisme rizosfer lebih tinggi (96%) dibandingkan mikroorganisme endofit (4%). Kepadatan bakteri dan jamur rizosfer masing-masing adalah 47x103 dan 2x103 CFU g-1 sedangkan kepadatan bakteri endofit dan jamur masing-masing hanya 2x103 dan 1x103 CFU g-1.
MAPPING OF SOIL DEGRADATION POTENCY IN PADDY FIELD WONOGIRI, INDONESIA Mujiyo Mujiyo; Rahayu Rahayu; Reni Ustiatik; Dhika Sri Anggrahini
Sains Tanah - Jurnal Ilmu Tanah dan Agroklimatologi Vol 13, No 1 (2016)
Publisher : Faculty of Agriculture, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15608/stjssa.v13i1.481

Abstract

Sustainability of paddy field becomes the main concern as the media of biomass production, thus it is needed a data and information about land characteristics to find out its degradation. Mapping of soil degradation potency in paddy field is an identification of initial soil condition to discover the land degradation potency. Mapping was done by overlaying map of soil, slope, rainfall and land use with standard procedures to obtain its value and status of soil degradation potency. Area mapping is an effective land for biomass production (natural forest, mixed farm, savanna, paddy field, shrub and dry field) with approximately 43,291.00 hectares (ha) in Sidoharjo, Girimarto, Jatipurno, Jatisrono, Jatiroto, Tirtomoyo, Nguntoronadi and Ngadirojo District. The result shows that soil degradation potency (SDP) in Districts of Sidoharjo, Girimarto, Jatipurno, Jatisrono, Jatiroto, Tirtomoyo, Nguntoronadi and Ngadirojo are very low (DP I) 0.00 ha (0.00%), low (DP II) 20,702.47 ha (47.82%), moderate (DP III) 15,823.80 ha (36,55%), high (DP IV) 6,764.73 ha (15.63%) and very high (DP V) 0.00 ha (0.00%). Paddy field is 22,036.26 ha or about 50.90% of all area as effective biomass production, its SDP considers as low (DP II) 16,021.04 ha (37.01%) and moderate (DP III) 6,015.22 ha (13,89%). Paddy field has a low SDP because it is commonly lies on flat area and conservation method by the farmer is maintaining the paddy bund and terrace. This study needs an advanced study to identify actual SDP through detail verification in the field, and also support by soil sample analysis in the laboratory. 
Kajian Bioinformatika Penghambatan Alosterik Asemanan Dan Glukomanan Terhadap C-JUN NH2 Terminal Kinase (JNK) Dewi Ratih Tirto Sari; Reni Ustiatik; Jatmiko Eko Witoyo; Gabriella Chandrakirana Krisnamurti; Yohanes Bare
SPIZAETUS: JURNAL BIOLOGI DAN PENDIDIKAN BIOLOGI Vol 2, No 2 (2021): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v2i2.47

Abstract

cJun NH2 terminal Kinase (JNK) merupakan protein kinase family MAPK yang berperan dalam j alur pensinyalan penyakit metabolisme, salah satunya dalam regulasi faktor resiko obesitas. Penelitian in bertujuan untuk mengeksplorasi potensi asemanan dan glukomanan dalam menghambat JNK sebagai antidiabetes.metode pendekatan molecular docking digunakan untuk mengidentifikasi interaksi antara senyawa asemanan dan glukomanan terhadap protein JNK. Asemanan dan glukomanan berikatan di sisi aktif yang berbeda satu sama lain. Residu sisi aktif asemanan berada di close gate protein JNK, sedangkan glukomanan menunjukkan sisi aktif jalur ikatan inhibitor dari JNK. Asemanan dan glukomanan menghambat aktivitas JNK dengan berikatan di sisi non-katalitik dan diprediksi penghambatan protein JNK oleh kedua senyawa secara alosterik yang dapat merubah konformasi protein JNK. Selain itu, asemanan berikatan dengan kuat terhadap protein JNK dengan jenis ikatan hydrogen, interaksi hidrofobik dan elektrostatik dengan energi ikatan yang lebih rendah dari glukomanan – JNK. Penelitian disimpulkan bahwa senyawa asemanan dan glukomanan berpotensi sebagai antiobesitas dengan peranannya sebagai inhibitor terhadap protein JNK.
Soil quality status under Hazton’s paddy farming: A case study in Banyumas Regency, Indonesia Supriyadi Supriyadi; Reni Ustiatik; Brilliant Mukti; Slamet Minardi; Hery Widijanto; Muchammad Bima Gegana Sakti
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 19, No 2 (2022): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v19i2.58375

Abstract

Soil quality is the ability of the soil to perform its function, such as providing nourishment to the plants. However, intensive paddy farming, such as Hazton’s paddy farming method, is suspected to deteriorate soil quality status and degrade land sustainability. This study aimed to analyze soil quality under Hazton’s paddy farming. This study was conducted on paddy fields in Banyumas Regency using a randomized block design with treatment consisting of 1) conventional method as a control, 2) Hazton’s method + organic fertilizer, 3) Hazton’s method + organic fertilizer + decomposer, and 4) Hazton’s method + organic fertilizer + decomposer + leaf fertilizer. Soil quality was determined according to a minimum data set (MDS) that consisted of organic C, pH, total N, available phosphorus (P) and potassium (K), base saturation (BS), cation exchange capacity (CEC), bacterial density, soil respiration, and C/N ratio. The MDS was scored and calculated using the soil quality index formula and then classified from very low to very high (<0.19-1). This study highlighted that the soil quality in paddy farm using Hazton’s method in Banyumas Regency ranged from low (0.444) to very low (0.308). The application of organic fertilizer is not sufficient enough to refill the nutrient pool equal to harvested plant biomass. This leads to soil quality deterioration and affects land sustainability. Therefore, yield and biomass production should be included as soil quality indicators in future studies. Additionally, further soil degradation can be avoided by continuously assessing soil quality and the necessary conservation measures for preventing and minimizing further land degradation can be applied.
Molecular Docking Approach Of Brewed Coffee Compounds As Neuroprotective Agent Dewi Ratih Tirto Sari; Reni Ustiatik; Siti Zamilatul Azkiyah
Natural Science Vol 9, No 1 (2023)
Publisher : Universitas Islam Negeri Imam Bonjol Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15548/nsc.v9i1.5778

Abstract

Coffee is the most popular beverage in the community and the main export commodity in Indonesia. coffee has a special flavor due to roasting and brewing techniques. This study identified the potential neuroprotective effect of eight compounds in brewed coffee through acetylcholinesterase inhibition by molecular docking. Molecular docking approach was conducted in this study. Eight compounds involved 2-Ethyl- 3,5 – dimethylpyrazine, Guaiacol, 2-Isobutyl-3- methoxypyrazine, 2- Methylpropanal, 3-Methyl – 2 – butene – 1 - thiol, 3- Methylbutanal, Furaneol, and 2 – Furfurylthiol were retrieved their structure from PubChem NCBI database and redocked with acetylcholinesterase by Molegro virtual docker version 5.0. then, the docking results were visualized and analyzed by PyMol 2.3 and Discovery Studio version 21.1.1. the docking studies performed that eight brewed coffee compound showed interaction with acetylcholinesterase at the protein gate and active sites. Even the interaction was not found in catalytic triad, the interaction of ligands – protein change the conformation and prevent the breaking down of acetylcholine to acetic acid and choline. This study suggested that eight brewed coffee compounds potentially as neuroprotective effect trough blocking acetylcholinesterase protein.
PENGARUH PENAMBAHAN ABU TERBANG BATU BARA DAN PUPUK KANDANG AYAM TERHADAP SERAPAN P DAN PERTUMBUHAN TANAMAN JAGUNG PADA ULTISOL Muhammad Miftakhul Falah; Reni Ustiatik; Yulia Nuraini
Jurnal Tanah dan Sumberdaya Lahan Vol. 10 No. 2 (2023)
Publisher : Departemen Tanah, Fakultas Pertanian, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtsl.2023.010.2.21

Abstract

Maize is one of the world's food crops besides wheat and rice, which is strategic to increase production. Ultisol is a type of soil spread across Indonesia with a land area distribution of 45,794,000 ha and has the potential to be used to increase corn crop production. This study aimed to elucidate the effect of the combination of coal fly ash (CFA) and chicken manure (CM) on P uptake and growth of maize grown on an Ultisol. The CFA doses applied were 0, 10 and 20 t ha-1 of coal fly ash, while the CM doses applied were 0, 10 and 20 t ha-1 of chicken manure. Observations included soil pH, total P, available P and P uptake by maize. The results of the study showed an increase in soil pH, but still showed a value below 5,5. The addition of coal fly ash and chicken manure as much as 20 t ha-1 provided the highest P availability and showed the highest P nutrient uptake value by corn crops. The application of coal fly ash and chicken manure can increase the availability of P better than without the addition of chicken manure. But, the application of coal fly ash up to 20 t ha-1 also inhibited the growth of maize by marking a decrease in the results of measuring the dry weight and height of maize plants.
Together with Farmers, Exploring Adaptive and Economically Valued VegetationPost-Eruption of Mount Semeru: Bersama Petani Menggali Vegetasi Adaptif dan Bernilai Ekonomi Pasca ErupsiGunungSemeru Kurniawan Sigit Wicaksono; Reni Ustiatik; Sri Rahayu Utami; Zaenal Kusuma; Mochtar Lutfi Rayes; Istika Nita
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 6 (2023): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v7i6.14655

Abstract

Mount Semeru eruption in 2020 and 2021 damaged 851 ha of productive land. Restoration of the affected lands requires information such as wide area, distribution, and eruption material characteristics. This community service aimed to provide a database as an overview of pyroclastic materials characteristics and elucidate potential-and-adaptive plants and microbes on post-Mt. Semeru eruptions. The volcanic ash contains significant elements (Al, Si, K, Ca, Ti, and Fe) and minor elements (V, Cr, Mn, Cu, Zn, Sr, Ba, Eu, and Re). Some of the plants that have survived post-eruption are annual shrubs and herbaceous plants such as grasses, so these plants can be used to restore the affected lands fertility after the Mt Semeru eruption. Based on economic evaluation and conditions in the field, the recommended adaptive plants are Pennisetum puparium and Musa paradisiaca. Both plants provide economic benefits for animal feed and farmers' income during post-eruption land recovery.
Application of Pb-Resistant Bacteria to Reduce Pb-Accumulation in Brassica sp. on Pb-Contaminated Soil Beauty Laras Setia Pertiwi; Yulia Nuraini; Sugeng Prijono; Reni Ustiatik
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 12, No 4 (2023): December 2023
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v12i4.863-874

Abstract

Brassica sp. is a horticultural crop with high demand for consumption. To meet the demand, farmers apply intensive farming to increase yields and prevent plant pests and diseases that cause yield loss. Agrochemical applications in the form of inorganic fertilizers and pesticides contribute to lead (Pb) contamination in agricultural soils and increase lead (Pb) content in the cultivated plants. Nowadays, using bacteria for remediation (bioremediation) is environmentally friendly and effective in cleaning pollutants by converting organic Pb into inorganic Pb which is less toxic. This study aims to explore Pb-resistant bacteria that can reduce Pb-accumulation on food crops such as Brassica sp. grown in Pb-contaminated soil. This study isolated 15 isolates that survived on nutrient agar containing 1,000 mg/L Pb(NO3)2. The study showed 2 potential Pb-resistant bacteria that reduced Pb accumulation in Brassica sp. up to 30.5%. The bacteria are gram-positive bacilli and non-human pathogens (PT-3 and PT-5). DNA barcode identification results showed the isolates identified as Bacillus altitudinis (PT-3) and Bacillus wiedmannii (PT-5). Aplication of the bacteria increases the shoot length and fresh weight of Brassica sp. Application of the bacteria improves food crops quality by reducing heavy metals accumulation, such as Pb. Thus, the bacteria are potential as biofertilizers for reducing agrochemicals use in intensive agriculture areas and preventing environmental destruction and food contamination. Keywords:   Bioremediation, Brassica sp., Inorganic Fertilizers, Lead-Resistant Bacteria, Pesticides. 
Farmers' Bussiness as Usual Increase Lead (Pb) Level in the Soil: a Case Study Horticulture Land in Batu, Indonesia Reni Ustiatik; Beauty Laras Setia Pertiwi; R. M. Yusuf Adi Pujo Nugroho; Georona Kusma Albarki; Siti Nur ‘Azizah; Ayu Putri Ariska; Yulia Nuraini; Syahrul Kurniawan
Jurnal Penelitian Pendidikan IPA Vol 10 No 1 (2024): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i1.4719

Abstract

Lead (Pb) is a toxic heavy metal for humans. Agrochemical applications contribute to Pb contamination and accumulation in plants which is harmful to humans. This study aims to investigate the habits of horticultural farmers in applying inorganic fertilizers and pesticides intensively on Pb concentration in the soil and cultivated plants, and its effect on plant growth. The measured parameters consisted of soil and plant Pb content, soil pH, organic C, total N, available P, and exchangeable K. This study used a randomized block design consisting of 4 treatments (Control (KT); (PR); (TN); and (TP)). The results showed that horticultural farmers' dosage of inorganic fertilizers exceeded Indonesia Ministry of Agriculture recommendation. The application of inorganic fertilizers and pesticides by farmers (business as usual) increased Pb content in plants up to 31.46 mg/kg. Besides the application of inorganic fertilizers increases fertilizer residues in the soil by up to 65%, which increases environmental pollution even though the application of inorganic fertilizers increases plant growth and production (shoot length,  the number of leaves, and plant dry weight). Thus, wisely applying agrochemicals is required in order to support plant growth and production without further environmental problems.
Development of Conservation Agriculture on Sloping Land for Potato Commodities in Wonokitri Village, Pasuruan, East Java: Pengembangan Pertanian Konservasi Pada Lahan Berlereng Untuk Komoditas Kentang di Desa Wonokitri, Pasuruan, Jawa Timur Istika Nita; Muhamad Taufiq Hidayat; Awang Satya Kusuma; Kurniawan Sigit Wicaksono; Lenny Sri Nopriani; Reni Ustiatik; Sativandi Riza; Sri Rahayu Utami; Syamsu Ridzal Indra Hadi; Anggraini Aurina Putri; Aksha Karunia Satria
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 3 (2024): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v8i3.19815

Abstract

Sistem pertanian pada dataran tinggi mempunyai keunikan dan tantangan tersendiri, termasuk kawasan pertanian di Desa Wonokitri, Pasuruan, Jawa Timur. Kesesuain lahan untuk budidaya komoditas hortikultura menyebabkan praktek pertanian yang digunakan termasuk intensif melalui pengolahan tanah, pola tanam, penggunaan pupuk hingga pengendalian hama penyakit. Komoditas utama pada lahan ini adalah kentang, selain syarat tumbuh terpenuhi juga dikarenakan nilai jual kentang yang tinggi. Namun, yang menjadi tantangan adalah praktek penanaman dilakukan pada lahan berlereng dan tidak disertai dengan penerapan tindakan konservasi. Kegiatan pengabdian masyarakat ini bertujuan untuk mengembangkan pertanian koservasi yang berbasis kearifan lokal pada lahan berlereng di Desa Wonokitri, Pasuruan, Jawa Timur. Metode yang digunakan mencakup observasi lapangan, analisis tanah, dan Focus Group Discussion (FGD) dengan petani setempat. Sifat tanah di lahan pertanian wonokitri termasuk dalam tekstur lempung berdebu, berat isi tanah 0,62 g cm-3, porositas tinggi (> 50%), permeabilitas cepat (>12,7 cm jam-1) dan kemantapan agregat sangat stabil sekali (>2 mm), sehingga secara fisik sangat baik dalam menopang pertumbuhan tanaman kentang. Namun, sistem pertanian berlerang tanpa konservasi yang dilakukan sangat berpotensi menurunkan produktivitas lahan di masa mendatang. Oleh sebab itu, berdasarkan kondisi aktual lahan, kesesuaian tanaman dan sosial ekonomi dirumuskan tindakan konservasi yang dapat diterima yakni dengan penanaman pada bedengan searah lereng yang dilengkapi dengan saluran pengelak dan saluran pembuangan air (SPA) serta bak penampung untuk menangkap limpasan permukaan dan sedimen. Pada setiap petak lahan ditanami tanaman penguat guna meningkatkan kestabilan lereng dan perlindungan lahan. Program ini menunjukkan potensi besar dalam meningkatkan kesadaran dan penerapan konservasi tanah oleh petani setempat
Co-Authors Aksha Karunia Satria Alda Inayah A. Hi. Usman Anggraini Aurina Putri Awang Satya Kusuma Ayu Putri Ariska Ayu Putri Ariska Azis, Novrianty Rizqi Bare, Yohanes Beauty Laras Setia Pertiwi Brilliant Mukti Cahyo Prayogo, Cahyo Dewi Ratih Tirto Sari Dewi Ratih Tirto Sari Dhika Sri Anggrahini Eko Handayanto Gabriella Chandrakirana Krisnamurti Georona Kusma Albarki Hery Widijanto Istika Nita Jatmiko Eko Witoyo Kurniawan Sigit Wicaksono Kurniawan Sigit Wicaksono Maydella Vista Putri Rinandy Mochtar Lutfi Rayes Mochtar Lutfi Rayes Muchammad Bima Gegana Sakti Muhamad Taufiq Hidayat Muhammad Miftakhul Falah Muhammad Taufik Hidayat Mujiyo Mujiyo Novryanti Rizqi Aziz R Muhammad Yusuf Adi Pujo Nugroho R. M. Yusuf Adi Pujo Nugroho Rahayu Rahayu Resa Kharisma Ramadhan Rizky Eka Putra, Rizky Eka Sativandi Riza Siti Nur ‘Azizah Siti Zamilatul Azkiyah Slamet Minardi Sri Rahayu Utami Sri Rahayu Utami Sugeng Prijono Suharjono Supriyadi Supriyadi Syahrul Kurniawan Syahrul Kurniawan Syamsu Ridzal Indra Hadi Wikan Agung Nugroho Yulia Nuraini Yulia Nuraini Yulia Nuraini Zaenal Kusuma