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MAPPING OF SOIL DEGRADATION POTENCY IN PADDY FIELD WONOGIRI, INDONESIA Mujiyo Mujiyo; Rahayu Rahayu; Reni Ustiatik; Dhika Sri Anggrahini
Sains Tanah - Jurnal Ilmu Tanah dan Agroklimatologi Vol 13, No 1 (2016)
Publisher : Faculty of Agriculture, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15608/stjssa.v13i1.481

Abstract

Sustainability of paddy field becomes the main concern as the media of biomass production, thus it is needed a data and information about land characteristics to find out its degradation. Mapping of soil degradation potency in paddy field is an identification of initial soil condition to discover the land degradation potency. Mapping was done by overlaying map of soil, slope, rainfall and land use with standard procedures to obtain its value and status of soil degradation potency. Area mapping is an effective land for biomass production (natural forest, mixed farm, savanna, paddy field, shrub and dry field) with approximately 43,291.00 hectares (ha) in Sidoharjo, Girimarto, Jatipurno, Jatisrono, Jatiroto, Tirtomoyo, Nguntoronadi and Ngadirojo District. The result shows that soil degradation potency (SDP) in Districts of Sidoharjo, Girimarto, Jatipurno, Jatisrono, Jatiroto, Tirtomoyo, Nguntoronadi and Ngadirojo are very low (DP I) 0.00 ha (0.00%), low (DP II) 20,702.47 ha (47.82%), moderate (DP III) 15,823.80 ha (36,55%), high (DP IV) 6,764.73 ha (15.63%) and very high (DP V) 0.00 ha (0.00%). Paddy field is 22,036.26 ha or about 50.90% of all area as effective biomass production, its SDP considers as low (DP II) 16,021.04 ha (37.01%) and moderate (DP III) 6,015.22 ha (13,89%). Paddy field has a low SDP because it is commonly lies on flat area and conservation method by the farmer is maintaining the paddy bund and terrace. This study needs an advanced study to identify actual SDP through detail verification in the field, and also support by soil sample analysis in the laboratory. 
Kajian Bioinformatika Penghambatan Alosterik Asemanan Dan Glukomanan Terhadap C-JUN NH2 Terminal Kinase (JNK) Dewi Ratih Tirto Sari; Reni Ustiatik; Jatmiko Eko Witoyo; Gabriella Chandrakirana Krisnamurti; Yohanes Bare
SPIZAETUS: JURNAL BIOLOGI DAN PENDIDIKAN BIOLOGI Vol 2, No 2 (2021): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v2i2.47

Abstract

cJun NH2 terminal Kinase (JNK) merupakan protein kinase family MAPK yang berperan dalam j alur pensinyalan penyakit metabolisme, salah satunya dalam regulasi faktor resiko obesitas. Penelitian in bertujuan untuk mengeksplorasi potensi asemanan dan glukomanan dalam menghambat JNK sebagai antidiabetes.metode pendekatan molecular docking digunakan untuk mengidentifikasi interaksi antara senyawa asemanan dan glukomanan terhadap protein JNK. Asemanan dan glukomanan berikatan di sisi aktif yang berbeda satu sama lain. Residu sisi aktif asemanan berada di close gate protein JNK, sedangkan glukomanan menunjukkan sisi aktif jalur ikatan inhibitor dari JNK. Asemanan dan glukomanan menghambat aktivitas JNK dengan berikatan di sisi non-katalitik dan diprediksi penghambatan protein JNK oleh kedua senyawa secara alosterik yang dapat merubah konformasi protein JNK. Selain itu, asemanan berikatan dengan kuat terhadap protein JNK dengan jenis ikatan hydrogen, interaksi hidrofobik dan elektrostatik dengan energi ikatan yang lebih rendah dari glukomanan – JNK. Penelitian disimpulkan bahwa senyawa asemanan dan glukomanan berpotensi sebagai antiobesitas dengan peranannya sebagai inhibitor terhadap protein JNK.
Soil quality status under Hazton’s paddy farming: A case study in Banyumas Regency, Indonesia Supriyadi Supriyadi; Reni Ustiatik; Brilliant Mukti; Slamet Minardi; Hery Widijanto; Muchammad Bima Gegana Sakti
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 19, No 2 (2022): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v19i2.58375

Abstract

Soil quality is the ability of the soil to perform its function, such as providing nourishment to the plants. However, intensive paddy farming, such as Hazton’s paddy farming method, is suspected to deteriorate soil quality status and degrade land sustainability. This study aimed to analyze soil quality under Hazton’s paddy farming. This study was conducted on paddy fields in Banyumas Regency using a randomized block design with treatment consisting of 1) conventional method as a control, 2) Hazton’s method + organic fertilizer, 3) Hazton’s method + organic fertilizer + decomposer, and 4) Hazton’s method + organic fertilizer + decomposer + leaf fertilizer. Soil quality was determined according to a minimum data set (MDS) that consisted of organic C, pH, total N, available phosphorus (P) and potassium (K), base saturation (BS), cation exchange capacity (CEC), bacterial density, soil respiration, and C/N ratio. The MDS was scored and calculated using the soil quality index formula and then classified from very low to very high (<0.19-1). This study highlighted that the soil quality in paddy farm using Hazton’s method in Banyumas Regency ranged from low (0.444) to very low (0.308). The application of organic fertilizer is not sufficient enough to refill the nutrient pool equal to harvested plant biomass. This leads to soil quality deterioration and affects land sustainability. Therefore, yield and biomass production should be included as soil quality indicators in future studies. Additionally, further soil degradation can be avoided by continuously assessing soil quality and the necessary conservation measures for preventing and minimizing further land degradation can be applied.
Molecular Docking Approach Of Brewed Coffee Compounds As Neuroprotective Agent Dewi Ratih Tirto Sari; Reni Ustiatik; Siti Zamilatul Azkiyah
Natural Science Vol 9, No 1 (2023)
Publisher : Universitas Islam Negeri Imam Bonjol Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15548/nsc.v9i1.5778

Abstract

Coffee is the most popular beverage in the community and the main export commodity in Indonesia. coffee has a special flavor due to roasting and brewing techniques. This study identified the potential neuroprotective effect of eight compounds in brewed coffee through acetylcholinesterase inhibition by molecular docking. Molecular docking approach was conducted in this study. Eight compounds involved 2-Ethyl- 3,5 – dimethylpyrazine, Guaiacol, 2-Isobutyl-3- methoxypyrazine, 2- Methylpropanal, 3-Methyl – 2 – butene – 1 - thiol, 3- Methylbutanal, Furaneol, and 2 – Furfurylthiol were retrieved their structure from PubChem NCBI database and redocked with acetylcholinesterase by Molegro virtual docker version 5.0. then, the docking results were visualized and analyzed by PyMol 2.3 and Discovery Studio version 21.1.1. the docking studies performed that eight brewed coffee compound showed interaction with acetylcholinesterase at the protein gate and active sites. Even the interaction was not found in catalytic triad, the interaction of ligands – protein change the conformation and prevent the breaking down of acetylcholine to acetic acid and choline. This study suggested that eight brewed coffee compounds potentially as neuroprotective effect trough blocking acetylcholinesterase protein.
PENGARUH PENAMBAHAN ABU TERBANG BATU BARA DAN PUPUK KANDANG AYAM TERHADAP SERAPAN P DAN PERTUMBUHAN TANAMAN JAGUNG PADA ULTISOL Muhammad Miftakhul Falah; Reni Ustiatik; Yulia Nuraini
Jurnal Tanah dan Sumberdaya Lahan Vol. 10 No. 2 (2023)
Publisher : Departemen Tanah, Fakultas Pertanian, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtsl.2023.010.2.21

Abstract

Maize is one of the world's food crops besides wheat and rice, which is strategic to increase production. Ultisol is a type of soil spread across Indonesia with a land area distribution of 45,794,000 ha and has the potential to be used to increase corn crop production. This study aimed to elucidate the effect of the combination of coal fly ash (CFA) and chicken manure (CM) on P uptake and growth of maize grown on an Ultisol. The CFA doses applied were 0, 10 and 20 t ha-1 of coal fly ash, while the CM doses applied were 0, 10 and 20 t ha-1 of chicken manure. Observations included soil pH, total P, available P and P uptake by maize. The results of the study showed an increase in soil pH, but still showed a value below 5,5. The addition of coal fly ash and chicken manure as much as 20 t ha-1 provided the highest P availability and showed the highest P nutrient uptake value by corn crops. The application of coal fly ash and chicken manure can increase the availability of P better than without the addition of chicken manure. But, the application of coal fly ash up to 20 t ha-1 also inhibited the growth of maize by marking a decrease in the results of measuring the dry weight and height of maize plants.
Application of Pb-Resistant Bacteria to Reduce Pb-Accumulation in Brassica sp. on Pb-Contaminated Soil Beauty Laras Setia Pertiwi; Yulia Nuraini; Sugeng Prijono; Reni Ustiatik
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 12, No 4 (2023): December 2023
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v12i4.863-874

Abstract

Brassica sp. is a horticultural crop with high demand for consumption. To meet the demand, farmers apply intensive farming to increase yields and prevent plant pests and diseases that cause yield loss. Agrochemical applications in the form of inorganic fertilizers and pesticides contribute to lead (Pb) contamination in agricultural soils and increase lead (Pb) content in the cultivated plants. Nowadays, using bacteria for remediation (bioremediation) is environmentally friendly and effective in cleaning pollutants by converting organic Pb into inorganic Pb which is less toxic. This study aims to explore Pb-resistant bacteria that can reduce Pb-accumulation on food crops such as Brassica sp. grown in Pb-contaminated soil. This study isolated 15 isolates that survived on nutrient agar containing 1,000 mg/L Pb(NO3)2. The study showed 2 potential Pb-resistant bacteria that reduced Pb accumulation in Brassica sp. up to 30.5%. The bacteria are gram-positive bacilli and non-human pathogens (PT-3 and PT-5). DNA barcode identification results showed the isolates identified as Bacillus altitudinis (PT-3) and Bacillus wiedmannii (PT-5). Aplication of the bacteria increases the shoot length and fresh weight of Brassica sp. Application of the bacteria improves food crops quality by reducing heavy metals accumulation, such as Pb. Thus, the bacteria are potential as biofertilizers for reducing agrochemicals use in intensive agriculture areas and preventing environmental destruction and food contamination. Keywords:   Bioremediation, Brassica sp., Inorganic Fertilizers, Lead-Resistant Bacteria, Pesticides. 
Lead (Pb)-Resistant Bacteria Improve Brassica chinensis Biomass and Reduce Pb Concentration in Pb-Contaminated Soil Pertiwi, Beauty Laras Setia; Ustiatik, Reni; Nuraini, Yulia
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86174

Abstract

Applications of inorganic fertilisers  and pesticides frequently increase lead (Pb) content in the soil and food crops. This study aims to isolate Pb-resistant bacteria and test the isolated bacteria in reducing Pb concentration and increasing biomass production of Brassica chinensis on Pb-contaminated soil.  Soil and plant samples were collected from agricultural land in Batu City, East Java, Indonesia. The isolated bacteria were tested for Pb resistance and then characterised  according to 16S rRNA Sequence. A pot trial with a completely randomised  block design consisting of 9 treatments and 3 replications was set to determine the effect of Pb-resistant bacteria inoculation on Pb residue, plant growth, and soil nutrients. The result showed that the isolated Pb-resistant bacteria were Bacillus wiedmannii and Bacillus altitudinis. The bacteria were resistant to Pb up to 10,000 mg/L PbNO3. Inoculation of the bacteria increased B. chinensis growth and biomass production, namely increasing the number of leaves (12%) and dry weight (35%).  Also, the bacteria reduced Pb residue in the soil by up to 88%. Moreover, soil essential nutrients such as total nitrogen, available phosphorus, and exchangeable potassium increased (12%, 73%, and 200%, respectively) after the application of Pb-resistant bacteria. The bacteria have the potential for bioremediation of Pb-contaminated soils on a large scale due to the bacteria prevent Pb uptake by food crops such as B. chinensis by reducing Pb content in the soil, which is good for food safety and environmental sustainability. 
Pengaruh Kombinasi Pupuk Organik dan Anorganik terhadap Sifat Fisik dan Kimia Tanah serta Produksi Padi pada Lahan Kering yang Disawahkan Putra, Rizky Eka; Rayes, Mochtar Lutfi; Kurniawan, Syahrul; Ustiatik, Reni
Agrikultura Vol 35, No 1 (2024): April, 2024
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v35i1.53686

Abstract

Lahan kering yang disawahkan memiliki proses pelumpuran dan penggenangan yang menyebabkan perbedaan kondisi sifat fisik tanah sehingga menurunkan kesuburan tanah, salah satunya yaitu kerusakan struktur tanah. Upaya untuk meningkatkan kesuburan tanah pada lahan tersebut dapat dilakukan dengan meningkatkan C-Organik, salah satunya yaitu aplikasi pupuk organik (asam amino dan asam humat). Aplikasi pupuk organik yang dikombinasikan pupuk anorganik dengan dosis yang tepat juga berpotensi untuk memperoleh produksi tanaman yang optimal. Tujuan penelitian ini yaitu untuk menganalisis perbedaan karakteristik tanah pada lahan kering yang disawahkan serta menganalisis pengaruh pemberian kombinasi pupuk organik dan anorganik terhadap kesuburan tanah dan produksi padi pada lahan kering yang disawahkan. Penelitian dilakukan di Kebun Percobaan Jatimulyo, Universitas Brawijaya. Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan 9 perlakuan dan 3 kali ulangan. Parameter yang diukur yaitu pH, C-Organik, kapasitas tukar kation (KTK), Kdd, kejenuhan basa, berat isi, tekstur, jumlah anakan produktif, dan berat gabah kering panen. Hasil penelitian menunjukkan terdapat perubahan sifat fisik dan kimia tanah akibat perubahan pengolahan dari lahan kering menjadi lahan sawah. Aplikasi pupuk organik dan anorganik memberikan pengaruh nyata terhadap sifat kimia seperti pH, C-Organik, N-total, P-tersedia, Kdd dan KTK. Aplikasi pupuk organik dan anorganik juga meningkatkan produksi padi sebesar 39-59% dengan perlakuan dosis terbaik yaitu 75% pupuk dasar anorganik + 100% pupuk organik.
Bio-physico-chemical Soil Characteristic: Intensive Tillage vs. No Tillage Reni Ustiatik; Ayu Putri Ariska; Resa Kharisma Ramadhan; Novryanti Rizqi Aziz; Syamsu Ridzal Indra Hadi; R Muhammad Yusuf Adi Pujo Nugroho; Maydella Vista Putri Rinandy; Muhammad Taufik Hidayat; Wikan Agung Nugroho; Syahrul Kurniawan
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 13, No 4 (2024): December 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i4.1196-1205

Abstract

Soil tillage has both positive and negative impacts on soil quality and crop productivity. Efforts to reduce the negative impacts of intensive soil tillage are urgently needed. This study aims to analyze the impact of intensive soil tillage on soil fertility parameters (pH, available P, organic-C, soil compaction, aggregate stability, and soil biodiversity). This research was conducted in two land uses: agriculture and forest land. The research design was descriptive-explorative through surveys and direct field observations. The sample points were determined using stratified random sampling with 3 replications (24 samples). Parameters analyzed in this study were soil compaction, aggregate stability, soil pH, soil available-P, and soil biodiversity (total microbial, soil meso-and-macrofauna). The results showed that intensive tillage affected the soil microbial population, aggregate stability, pH, and available-P (p<0.05). The negative impact of intensive soil tillage reduced total soil microbes by 59.37%. The soil macro and mesofauna found at the study site were earthworms and mycorrhizae, which had a higher density on non-tillage land, with trees as the main vegetation. This encourages efforts to introduce conservation soil tillage to maintain soil biodiversity before more severe damage occurs. Keywords: Intensive agriculture, Soil degradation, Soil fertility, Soil quality, Soil structure.
Effectiveness of Various Types of Manure and Inorganic Fertilizers on Populations of N-fixing and P-Solubilizing Bacteria and Nutrient Uptake of Maize in Inceptisol Alda Inayah A. Hi. Usman; Reni Ustiatik; Sri Rahayu Utami; Yulia Nuraini
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 13, No 3 (2024): September 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i3.880-889

Abstract

Bacteria are classified as non-symbiotic N-fixing and P-solubilizing play an important role in enhancing soil quality and plant growth. This study aims to analyze various types of manures and inorganic fertilizers on N-fixing and P-solubilizing bacteria population and their relationship with N and P uptake of maize in Inceptisol. The manure used was poultry, goat, and cow manure with doses of 0 t/ha, 10 t/ha, and 20 t/ha, respectively, and inorganic fertilizer NPK Phonska 15-15-15 dose 0 kg/ha; 150 kg/ha (50% of recommendation dose) and 300 kg/ha (100% of recommendation dose). Application of manure (poultry, goat, and cow manure) increased N-fixing and P-solubilizing bacteria population. The application of various types of manure and inorganic fertilizers has a significant impact on N and P uptake. The highest N and P uptake were 29.92 kg/ha and 2.63 kg/ha, respectively. Manure application can reduce inorganic fertilizer dose by up to 50%, increasing plant production efficiency and environmental sustainability. Keywords: Bacteria population, Fertilizer optimization, Inceptisol, Nutrient uptake.