Kalpataru
Vol. 31 No. 1 (2022)

Prehistoric Human Forensic DNA Analysis From Lore Highlands, Central Sulawesi With Present Day Human DNA

Najlaa Illiyyien Hadid (Faculty of Biology, University of Gadjah Mada, Yogyakarta, Indonesia)
Indra Lesmana (Faculty of Biology, University of Gadjah Mada, Yogyakarta, Indonesia)
Niken Satuti Nur Handayani (Faculty of Biology, University of Gadjah Mada, Yogyakarta, Indonesia)
Dwi Yani Yuniawati Umar (Research Center for Prehistoric and Historical Archaeology, BRIN, Indonesia)



Article Info

Publish Date
30 Jun 2022

Abstract

Mitochondrial DNA or mtDNA(s) are inherited maternally, in other words they can only be inherited through females in a family, which makes them frequently used in forensic studies. In this research, the Hypervariable-I part (HV-1) in the D-Loop region of the ancient forensic samples acquired from Lore Highlands will be compared with present day human DNA from the same region. The steps conducted in this research includes DNA extraction, mtDNA amplification with specific primers, sequencing, and phylogenetic analysis. The analysis were done between 4 ancient forensic samples; A, B, G, and J, and 33 comparison sequences from GenBank, including ancient forensic sample acquired from Tadulako site, Lore Highlands, from previous research. The result of genetic distance analysis showed that the distance between 37 samples were very close; with difference ranging from 0,02% - 0,13%. The analysis also gives a clue about Austronesian relation with Australomelanesian. The result from phylogenetic tree reconstructions (maximum-likelihood and neighbor-joining) showed little differences. However, there is a small significant difference detected from the neighbour-joining tree construction result and will be discussed further in this paper.

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Journal Info

Abbrev

kalpataru

Publisher

Subject

Arts Humanities Environmental Science Physics Social Sciences

Description

KalpataruĀ is an open access and peer reviewed scientific publication on the prehistory of Southeast Asia and its surrounding areas with the scope of materials such as culture, humans, and environment during the prehistoric and proto historic periods. The perspectives from cross disciplines other ...