Effect of Topical Application of Black Seed Oil (Nigella Sativa) on Wound Healing in Diabetic Rat Ulcer Model

Eliza Arman (Faculty of Farmacy, Andalas University, Padang, Indonesia/ Department of Medical Laboratory Technology, STIKES Syedza Saintika, Padang, Indonesia)
Almahdy A (Faculty of Farmacy, Andalas University, Padang, Indonesia)
Putri Dafiriani (Department of Biomedical, STIKES Syedza Saintika, Padang, Indonesia)
Dedy Almasdy (Faculty of Farmacy, Andalas University, Padang, Indonesia)

Majalah Kedokteran Andalas
Vol 46, No 10 (2024): Supplementary April 2024

Objective : This study aims to evaluate the use of topical black seed oil on wound healing in a diabetic rat model. Method:10 experimental rats were induced with streptozotocin at a dose of 55 mg/kg BW. Diabetic rats were anesthetized and then the wound was made with a biopsy push with a diameter of 10 mm. The rats were divided into 2 groups, namely the untreated group and the topical treatment group with 1 ml of 100% Black Seed Oil every day for 14 days. Wound healing evaluation was carried out on days 7 and 14. Gene expression analysis was performed using molecular technique RT PCR an histopathologically using He staining. Data analysis used T test. Result: On day 7 there was a decrease in TNF expression with p <0.05, while on day 14 it also showed a decrease in TNF alpha expression with p <0.01. Histopathological showed positive development by showing better wound healing with complete epithelialization, granulation with lower inflammatory cells, higher fibroblasts and collagen compared to the control group. Conclusion :Black seed oil can increase the speed of wound healing in diabetic rat models by inhibiting TNF alpha expression.