Recovery of vitamin D levels by cholecalciferol supplementation on obese rats

Veonika, Dea Anenta (Sebelas Maret University)
Wiboworini, Budiyanti (Sebelas Maret University)
Muthmainah, Muthmainah (Univerisitas sebelas Maret)

Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics)
VOLUME 12 ISSUE 1, 2024

ABSTRAKLatar Belakang: Obesitas disebabkan oleh penumpukan lemak dalam tubuh karena faktor biologis, psikososial, dan perilaku. Prevalensi obesitas mencapai 42,24% di Amerika Serikat, sementara overweight dan obesitas mencapai 59% di Eropa. Obesitas dapat menyebabkan defisiensi vitamin D melalui berbagai mekanisme.Tujuan: Untuk mengatahui pengaruh pemberian suplementasi cholecalciferol terhadap kadar 25(OH)D pada tikus obesitas. Metode: Dalam penelitian ini, kami melakukan penelitian  true experiment with.        pre-post test control group design. Penelitian ini menganalisis 3 kelompok tikus galur Sprague Dawley jantan yang diinduksi dengan diet tinggi lemak tinggi fruktosa (HFHF)  menjadi tikus obesitas. Masing-masing kelompok mendapatkan cholecalciferol sebanyak 2,500 IU/200gr/hari pada kelompok P1, 5,000IU/200gr/hari pada kelompok P2, dan 10,000 IU/200gr/hari pada kelompok P3 selama 8 minggu. Tikus kemudian dianalisis kadar serum 25(OH)D sebelum dan sesudah perlakuan.Hasil: Suplementasi cholecalciferol secara signifikan meningkatkan kadar vitamin D pada setiap kelompok intervensi yang diberikan cholecalciferol. Rerata kadar 25(OH)D kelompok P1,P2, dan P3 sebelum perlakuan  berturut-turut adalah 29,43±0,83 ng/mL, 28,61±1,57 ng/mL, dan 28,86±1,46 ng/mL. Rerata kadar 25(OH)D setelah suplementasi cholecalciferol untuk kelompok P1,P2, dan P3 berturut-turut adalah 74,27±0,77 ng/mL, 100,30±1,48 ng/mL, dan 126,73±2,30 ng/mL. Ada perbedaan yang signifikan antara nilai 25(OH)D sebelum dan sesudah perlakuan  pada ketiga kelompok intervensi dengan nilai (p<0,05). Kesimpulan: Pemberian cholecalciferol mampu meningkatkan kadar 25(OH)D pada tikus jantan yang diinduksi obesitas. KATA KUNCI: cholecalciferol; obesitas; vitamin D ABSTRACT Background: Obesity is caused by the accumulation of fat in the body due to biological, psychosocial and behavioral factors. The prevalence of obesity reaches 42.24% in the United States, while overweight and obesity reaches 59% in Europe. Obesity can cause vitamin D deficiency through various mechanisms.Objectives: To determine the effect of cholecalciferol supplementation on 25(OH)D levels in obese mice.Methods: In this research, we conducted a true experiment research with pre-post test control group design. This study analyzed 3 groups of male Sprague Dawley rats that were induced by a high-fat, high-fructose (HFHF) diet to become obese rats. Each group received 2,500 IU/200gr/day of cholecalciferol in group P1, 5,000IU/200gr/day in group P2, and 10,000 IU/200gr/day in group P3 for 8 weeks. The mice were then analyzed for serum 25(OH)D levels before and after treatment.Results: Cholecalciferol supplementation significantly increased vitamin D levels in each intervention group given cholecalciferol. The mean 25(OH)D levels in groups P1, P2, and P3 before treatment were 29.43 ± 0.83 ng/mL, 28.61 ± 1.57 ng/mL, and 28.86 ± 1.46, respectively. ng/mL. The mean 25(OH)D levels after cholecalciferol supplementation for groups P1, P2, and P3 were 74.27±0.77 ng/mL, 100.30±1.48 ng/mL, and 126.73±2 respectively. .30 ng/mL. There was a significant difference between the 25(OH)D values before and after treatment in the three intervention groups with values (p<0.05).Conclusion: Administration of cholecalciferol can increase 25(OH)D levels in male mice that are induced by obesity. KEYWORDS: cholecalciferol, vitamin D, obesity