Jurnal Natural
Volume 18, Number 2, June 2018

MOLECULAR IDENTIFICATION OF SHOREA JOHORENSIS IN KETAMBE RESEARCH STATION, GUNUNG LEUSER NATIONAL PARK

Nir Fathiya (Master Program of Biology, Biology Department, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Jalan Tgk Chik Pante Kulu No. 5, Banda Aceh 23111, Indonesia)
Essy Harnelly (Biology Department, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Jalan Tgk Chik Pante Kulu No. 5, Banda Aceh 23111, Indonesia)
Zairin Thomy (Biology Department, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Jalan Tgk Chik Pante Kulu No. 5, Banda Aceh 23111, Indonesia)
Iqbar Iqbar (Biology Department, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Jalan Tgk Chik Pante Kulu No. 5, Banda Aceh 23111, Indonesia)



Article Info

Publish Date
06 Jun 2018

Abstract

Shorea johorensis is one of the red meranti plants in Ketambe Research Station, Gunung Leuser National Park, Aceh Tenggara. Currently, Shorea johorensis also is well known as a major source of valuable commercial timber. This research aimed to analyze the phylogenetic of Shorea johorensis based on chloroplast and nuclear DNA in Ketambe Research Station so that it can be known the relationship of Shorea johorensis with other species Dipterocarpaceae in GenBank database. The research was conducted from July 2015 to August 2016 in Ketambe Research Station and Forestry and Forest Genetics Laboratory of Molecular, Bogor Agricultural University. The method used quadrat sampling technique with purposive sampling and experimental laboratory that consisted of DNA extraction, PCR, electrophoresis, and sequencing. The data analysis was done using BioEdit, MEGA6, and BLAST. The results showed that the phylogenetic tree of Shorea johorensis based on the rbcL and matK showed that Shorea johorensis was closely related with some species Hopea; but the phylogenetic tree based on psbA-trnH, 5.8S rRNA, ITS2, and 28S rRNA showed that Shorea johorensis was closely related with Shorea robusta.Keywords: Chloroplast dna, Ketambe Research Station,Nuclear DNA, Shorea johorensis

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Journal Info

Abbrev

natural

Publisher

Subject

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Immunology & microbiology Neuroscience Physics

Description

Jurnal Natural (JN) aims to publish original research results and reviews on sciences and mathematics. Jurnal Natural (JN) encompasses a broad range of research topics in chemistry, pharmacy, biology, physics, mathematics, statistics, informatic and ...