Poltekita : Jurnal Ilmu Kesehatan
Vol. 17 No. 4 (2024): February

Detection of Dengue Virus in Female Aedes aegypti Mosquito using Reverse Transcription-polymerase Chain Reaction (RT-PCR) in West Jakarta

Paiman, Dessy (Unknown)
Setiani, Ony (Unknown)
Budiyono, Budiyono (Unknown)



Article Info

Publish Date
11 Feb 2024

Abstract

Dengue hemorrhagic fever (DHF) is an acute disease caused by dengue virus infection which is a clinical manifestation of bleeding, transmitted through the bite of a female mosquito Aedes aegypti. The incidence of dengue fever is still a health problem in developing countries, including Indonesia. There are several ways to detect the presence of dengue virus, namely by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The purpose of this study was to detect Dengue virus in adult female Aedes aegypti mosquitoes using RT-PCR. The type of research is a descriptive survey to determine the number of dengue virus transmission in Aedes aegypti mosquitoes in Kembangan Village, West Jakarta, especially in RW02 RT 1-10. There are 62 Aedes aegypties found in 100 houses that were selected by simple random sampling. Totally 23 female Aedes aegypties were identified from a total of 62. Moreover, the result showed that all Aedes aegypti mosquitoes caught in were negative for dengue virus. There are several reasons for the limitation of RT-PCR that caused zero findings, including potential false negatives, sensitivity, low quality control measures. Moreover, the small sample size and seasonal reason also played a role in impacting the zero result.

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Journal Info

Abbrev

JIK

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Nursing Public Health

Description

Poltekita : Jurnal Ilmu Kesehatan (e-ISSN: 2527-7170, p-ISSN: 1907-459X) is a peer-reviewed open access scientific journal published by Poltekkes Kemenkes Palu. The scope for Poltekita: Jurnal Ilmu Kesehatan includes nutrition, midwifery, nursing, public health, environmental health, food, and ...