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All Journal Poltekita : Jurnal Ilmu Kesehatan
Dessy Paiman
Sekolah Kejuruan Keperawatan, Jayapura
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Nursing Public Health

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Detection of Dengue Virus in Female Aedes aegypti Mosquito using Reverse Transcription-polymerase Chain Reaction (RT-PCR) in West Jakarta Paiman, Dessy; Setiani, Ony; Budiyono, Budiyono
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 4 (2024): February
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i4.3471

Abstract

Dengue hemorrhagic fever (DHF) is an acute disease caused by dengue virus infection which is a clinical manifestation of bleeding, transmitted through the bite of a female mosquito Aedes aegypti. The incidence of dengue fever is still a health problem in developing countries, including Indonesia. There are several ways to detect the presence of dengue virus, namely by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The purpose of this study was to detect Dengue virus in adult female Aedes aegypti mosquitoes using RT-PCR. The type of research is a descriptive survey to determine the number of dengue virus transmission in Aedes aegypti mosquitoes in Kembangan Village, West Jakarta, especially in RW02 RT 1-10. There are 62 Aedes aegypties found in 100 houses that were selected by simple random sampling. Totally 23 female Aedes aegypties were identified from a total of 62. Moreover, the result showed that all Aedes aegypti mosquitoes caught in were negative for dengue virus. There are several reasons for the limitation of RT-PCR that caused zero findings, including potential false negatives, sensitivity, low quality control measures. Moreover, the small sample size and seasonal reason also played a role in impacting the zero result.
Co-Authors Budiyono Budiyono Daniel Lantang SATRIYAS ILYAS Setiani, Ony