Abstract The aim of the research is looking for probiotic bacteria strategy for depresing the growth of patogenic bacteria in digestion of grouper fish. Some of anti bacteria can be produced by bacteria, such as protease, bacteriocia (germicidine, pyrocydia, circulin), amilase lipase, and lyzozym. In this case, Bacillus sp. is chosen because it can produce antibacteria againts Vibrio sp. and probiotic multifactor effect, that is enzyme production and competition of nutrient and space.
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