Rafflesia Journal of Natural and Applied Sciences (RJNAS)
Vol. 2 No. 2 (2022): Oktober

Analysis of total phenolics and flavonoids content from methanol extract of Caesalpinia bonduc (L.) Roxb. seeds and antioxidant activity assay

Samriani Samriani (Universitas Hasanuddin)
Hasnah Natsir (Universitas Hasanuddin)
Seniwati Dali (Universitas Hasanuddin)
Abdul Wahid Wahab (Universitas Hasanuddin)
Nunuk Hariani Soekamto (Universitas Hasanuddin)
Paulina Taba (Universitas Hasanuddin)



Article Info

Publish Date
31 Oct 2022

Abstract

Caesalpinia bonduc (L.) Roxb. seed is known as gorek seed contain secondary metabolites such as alkaloids, tannins, flavonoids, and phenolics, due to the content of these secondary metabolites so that this plant has antioxidant activity. This study aimed to determine total phenolic and flavonoid contents of methanol extract of gorek C. bonduc seeds as well as antioxidant activity. Extraction of gorek C. bonduc seeds was carried out with methanol, then quantitative determination of total phenolic by the Folin-Ciocalteu method as Gallic Acid Equivalent (GAE)/gram of extract, flavonoid content by AlCl3 method as Quercetin Equivalent (QE)/gram of extract, and in vitro antioxidant activity with DPPH (2,2-diphenyl-1-picrylhydrazyl) method was in IC50 (inhibition concentration). Based on the results of research, the phytochemical screening of methanol extract of gorek C. bonduc seeds showed alkaloids, flavonoids, saponins, tannins, and phenolics. The determination of the total phenolic and flavonoid content were obtained 0.55 ± 0.02 mgGAE/g and 0.50 ± 0.02 mgQE/g, respectively. The antioxidant activity of methanol extracts and ascorbic acid showed IC50 values were 3770.77 ppm and 3.36 ppm respectively.

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Journal Info

Abbrev

rjna

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Medicine & Pharmacology Public Health

Description

Rafflesia Journal of Natural and Applied Sciences (RJNAS) with e-ISSN: 2808-3806 is an open access and peer reviewed journal published by Department of Chemistry, Universitas Bengkulu, Indonesia. This journal is published two times per year (April and October). RJNAS publishes research papers, ...