Jurnal Medika Veterinaria
Vol 14, No 1 (2020): J.Med.Vet

12. Antibacterial Activity of Ethanol Extract of Siamih Leaf (Ageratum conyzoides) on Staphylococcus aureus bacteria

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Herrialfian Herrialfian (Laboratorium Biokimia Fakultas Kedokteran Hewan Universitas Syiah Kuala)
M. Isa (Laboratorium Biokimia Fakultas Kedokteran Hewan Universitas Syiah Kuala)
Darmawi Darmawi (Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala)
Fakhrurrazi Fakhrurrazi (Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Syiah Kuala)
Abdul Harris (Laboratorium Farmakologi Fakultas Kedokteran Hewan Universitas Syiah Kuala)



Article Info

Publish Date
16 Jul 2020

Abstract

The aim of this research is to know the effect of siamih leaf ethanol extract (Ageratum conyzoides) on the growth of Staphylococcus aureus bacteria. The bacteria which is  used are obtained from the Microbiology Laboratory of the Faculty of Veterinary Medicine of Syiah Kuala University on isolate bacteria of the honey bear wound (Helarctos malayanus). Bacterial identification is done by culturing the isolate on lateral NA media then it is planted on MSA media and  staining Gram, catalase test and hemolysis test. The extraction is done by maceration with 96% ethanol solvent. Extract of Siamih leaf  is done by phytochemical test. The results of pythritic test of Siamih leaf show that Siamih leaf contains alkaloid compounds, steroids, terpenoids, flavonoids and tannins. The extract concentration which is used is 20%, 40%, 60% and 80%. Nergative control by using aquades and positive controls using Amoxicillin with three repetitions. The method used is disc diffusion or Kirby-Bauer method. The results show that Siamih leaf ethanol extract inhibit the growth of Staphylococcus aureus bacteria at concentrations of 20% (0,7 mm), 40% (1,4 mm), 60% (2,2 mm), and 80 % (3,0 mm). Thus it can be concluded that the extract of siamih leaf ethanol has inhibitory power to Staphylococcus aureus bacteria with low category.

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Journal Info

Abbrev

JMV

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Description

Jurnal Medika Veterinaria is published biannually in February and August. The scope of this journal includes Veterinary Medicine, Animal Science, Science, and Medicine. The journal accepts original research papers, case study, and review article. All submissions are evaluated by peer reviewers who ...