Biosaintifika: Journal of Biology & Biology Education
Vol. 16 No. 2 (2024): August 2024

DNA Barcoding of Ornamental Crab Geosesarma in South-Slope Mount Slamet Central Java, Indonesia

Elly Tuti Winarni (Faculty of Biology, Jenderal Soedirman University. Jl. dr. Soeparno 63 Grendeng, Purwokerto 53122, Central Java, Indonesia)
Aswi Andriasari Rofiqoh (Faculty of Biology, Jenderal Soedirman University. Jl. dr. Soeparno 63 Grendeng, Purwokerto 53122, Central Java, Indonesia)
Dian Bhagawati (Faculty of Biology, Jenderal Soedirman University. Jl. dr. Soeparno 63 Grendeng, Purwokerto 53122, Central Java, Indonesia)
Anastasia Endang Pulungsari (Faculty of Biology, Jenderal Soedirman University. Jl. dr. Soeparno 63 Grendeng, Purwokerto 53122, Central Java, Indonesia)
Hanan Hassan Alsheikh Mahmoud (College of Natural Resources and Environmental Studies, Department of Fisheries Science, University of Bahri Khartoum, 12217 Sudan)
Agus Nuryanto (Faculty of Biology, Jenderal Soedirman University. Jl. dr. Soeparno 63 Grendeng, Purwokerto 53122, Central Java, Indonesia)



Article Info

Publish Date
15 Aug 2024

Abstract

Geosesarma shows intraspecific carapace color variation, which might lead to species misidentification. The problem can be solved using DNA barcoding. There is one research about Geoserarma from the southern slopes of Mount Slamet, but samples were only collected from the Banjaran River for morphological identification. Here, we collected samples from wider areas covering south slope and applied molecular identification. This research aims to assess Geosesarma diversity in south-slope Mount Slamet Central Java, Indonesia based on the cytochrome c oxidase 1 gene barcoding. Surveys were carried out at six sites. Taxonomic identification was done using the barcoding technique. Four morphotypes were obtained during the research. Three morphotypes with the square carapace were identified as Geosesarma, while the remaining one morphotype was included in Parathelphusa. The three Geosesarma morphotypes were barcoded as Geosersarma dennerle because their genetic identity was more than 97% of the G. dennerle sequence in Boldsystems. In contrast, the Parathelphusa morphotype was barcoded as P. convexa with a genetic identity of 97.50%. It can be concluded that the Geosesarma crab on the south-slope Mount Slamet only consists of one species but has carapace and claw color variations. The data are essential for Geosesarma market development and conservation in the region.

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Journal Info

Abbrev

biosaintifika

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Biosaintifika: Journal of Biology & Biology Education {PISSN 2085-191X| EISSN 2338-7610} published scientific papers on the results of biology and biology education research {see Focus and Scope}. Editor accepts the article has not been published in other media with the writing format as listed on ...