DETECTION OF CRYPTOSPORIDIUM SPECIES IN CATTLE AND HANDLERS BY MOLECULAR TECHNIQUE IN WASIT PROVINCE

Murtadha Kadhim Hasan (a:1:{s:5:"en_US"s:79:"Department of Biology College of science for women University of Babylon ,Iraq"})
Zahraa Hamid Mohan (Department of Anatomy, Hammurabi College of Medicine, University of Babylon ,Iraq)
Farah Mohammed Saeed (Department of Biology College of science for women University of Babylon ,Iraq)
Oras Abdulsayed Mahdi (Department of Biology College of science for women University of Babylon ,Iraq)
Rasha Hamzah Abdulhussein (Pediatric Department, Hammurabi College of Medicine, University of Babylon, Iraq)
Sarah Hussein hamzah (Department of Biology College of science for women University of Babylon ,Iraq)
Mustafa basim Kareem (Alkut hospital for Gynecology and Pediatrics Iraq wasit)

JMGCB
Vol. 1 No. 3 (2024): Journal of Medical Genetics and Clinical Biology

I collected 300 cow feces samples (130 males - 170 females) and used molecular analysis to detect the parasite. The results showed significant differences (p ≤ 0.05) between the infection rate using particle technology of 38% (38/100) and 41% (41/100) in cows and breeders, respectively, while traditional microscopic methods showed 21% (21/100) and 17% (17/100) in cows and breeders, respectively. DNA sequence analyzes of 10 isolates from infected cows identified four species of the Cryptosporidium parasite genus which included: C. parvum (6/10), C. andersoni (2/10), C. bovis (1/10) and C. ryanae (1/10. The species C. andersoni, C. parvum, C. bovis and C. ryanae have been genetically confirmed through DNA sequence analysis, and it is considered the first recording of these species in local cattle in Iraq. In breeders, three types of the Cryptosporidium parasite were recorded through DNA sequence analysis in 10 isolates from infected individuals, which included: C. parvum (2/10), C. andersoni (5/10) and C. hominis (3/10). It is considered the first recording of this species among breeders in Iraq.