ROLE OF CYTOTOXIC T LYMPHOCYTE IN ADENOVIRUS INFECTION

Yasameen Waleed AL-Abedi (College of science Department of forensic science)
Ali Abdulhussein Alsaeedi (Medical Laboratory Technique Department, Kut University College, Al Kut, Wasit, Iraq)
Alaa Abdalhadi Halboti (Medical Laboratory Technique Department, Kut University College, Al Kut, Wasit, Iraq)

JMGCB
Vol. 1 No. 3 (2024): Journal of Medical Genetics and Clinical Biology

The successful development of adenovirus vectors for vaccines and gene therapy will require a better understanding of the host immune response. Using the ELISPOT assay to measure IFN-g-secreting CD8þ cells, we identify immunodominant epitopes of the adenovirus hexon and DNA-binding protein in BALB/c and C57BL/6 mice. The T-cell response to the intramuscular administration of adenovirus serotype 5 peaks within a few weeks and gradually declines but is still detectable after 12 weeks. A second administration did not substantially increase the number of reactive T cells. The CD8þ T-cell response was also similar between wild type and E1-deleted adenovirus. When B-cell-deficient mice were injected with adenovirus encoding the gene for secreted alkaline phosphatase, sera phosphatase activity was reduced more quickly in mice pre-exposed to adenovirus. These results add to the evidence that cell-mediated immunity is a substantial barrier to therapeutic adenoviral vectors and provide more quantitative tools to measure cellular immune responses to adenovirus.