DETECTION OF OQXAB EFFLUX PUMPS GENE IN ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIA ISOLATED FROM PREGNANT WOMEN SUFFERED FROM URINARY TRACT INFECTIONS AND DIABETES MELLITUS IN BASRAH

Amna Ali Nasir (Department of Biology, College of Science, University of Basrah, Iraq)
Adnan A.Al-Mousawi (Department of Biology, College of Science, University of Basrah, Iraq)
Zainab R. Abdul-Hussein (Department of Pathological analysis, College of Science, University of Basrah, Iraq)

JMGCB
Vol. 1 No. 4 (2024): Journal of Medical Genetics and Clinical Biology

Considerably, pregnant women with diabetes mellitus are highly susceptible to urinary tract infections. The aim of the study is investigate the incidence of OqxAB efflux pumps gene among Escherichia coli and Klebsiella pneumonia isolates from urine of pregnant women suffered from urinary tract infections and Diabetes mellitus. E.coli and K.pneumonia were identified using conventional methods and confirmed by specific 16S rDNA primers. Antibiotic susceptibility test of E. coli and K. pneumonia isolates against several antibiotics were examined using Vitek®-2 compact system .The production of efflux pump by E. coli and K. pneumonia isolates were investigated phenotypically using ethidium bromide-agar cartwheel method (EtBr CW). Moreover, OqxAB Efflux Pumps genes were tested. The results showed that among 100 urine samples, 40 urine samples showed positive bacterial growth where 21(51%) E coli isolates, 8(20%) K. pneumonia isolates. In terms of antibiotic sensitivity, all E.coli and K.pneumonia isolates were resistant to ampicillin, piperacillin and ticarcillin, respectively. The prevalence of OqxAB efflux pump genes frequency ranges from 72% in E. coli and 50% in K. pneumonia.