Chemical investigation on Pseudoceratina purpurea collected from Banyuwangi Indonesia

Triana Hertiani (Bagian Biologi Farmasi,Fakultas Farmasi UGM Jl. Sekip Utara Yogyakarta,55281)
RuAngelie Edrada (Institut für Pharmazeutische Biologie and Biotechnologie, Henrich-Heine Universität Düsseldorf, Universitätsstrasse 1 Geb. 26.23, 40225, Düsseldorf, Germany)
Rob W.M. Van Soest (Institute for Systematics and Ecology, University of Amsterdam, P.O. Box 94766, 1090 GT Amsterdam, The Netherlands)
Werner E.G. Muller (Institut für Physiologische Chemie und Pathobiochemie, Johannes-Guttenberg Universität, Duesbergwerg 6, 55128 Mainz, Germany.)
Sudarsono . (Pharmaceutical Biology Dept., Faculty of Pharmacy, Gadjah Mada University, Sekip Utara, 55281 Jogjakarta, Indonesia)
Peter Proksch (Institut für Pharmazeutische Biologie and Biotechnologie, Henrich-Heine Universität Düsseldorf, Universitätsstrasse 1 Geb. 26.23, 40225, Düsseldorf, Germany)

INDONESIAN JOURNAL OF PHARMACY
Vol 20 No 1, 2009

Chemical investigation on marine sponge Pseudoceratina purpurea collected from Banyuwangi, Indonesia has been performed. This study was aimed to isolate and to identify structures of the sponge secondary metabolites as well as to test their cytotoxicity activity on mouse lymphoma cell line L5178Y.Isolation procedure was performed by using different chromatography techniques. NMR spectroscopy and mass spectrometry methods were used to identify the compounds chemical structures. Cytotoxicity of the isolates was tested on mouse lymphoma cell line L5178Y by using the microculture tetrazolium (MTT) assay.This study yielded five known tyrosine-derived alkaloids, two of which, aplysamine-2 (1) and aeroplysinin-1 (2) showed growth inhibition of mouse lymphoma cell line L5178Y with IC50 value of 1.7 mg/mL and 0.57 mg/mL, respectively.Key words: Pseudoceratina purpurea, sponge, alkaloids.