Isolation of bacterial pathogen Escherichia coli o157:h7 in seafood samples and detection of flich7 gene by using PCR

Marlina . (Faculty of Pharmacy Andalas University, Padang Indonesia)
Elidahanum Husni (Faculty of Pharmacy Andalas University, Padang Indonesia)
Fitri Amalinda (Faculty of Pharmacy Andalas University, Padang Indonesia)
Son Radu (Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Selangor, Malaysia)
Mitsuaki Nishibuchi (Center for Southeast Asian Studies, Kyoto University, Kyoto 606-8501, Japan)

INDONESIAN JOURNAL OF PHARMACY
Vol 20 No 2, 2009

Escherichia coli O157:H7 is an important human pathogen causing haemorrhagic colitis, haemolytic uraemic syndrom and thrombotic thrombocytopenic purpura.In this study, P. pelagicus dan P. merguensis samples were collected from markets in Padang from Sept 2007 until Feb 2008. For isolation of the bacteria, samples were firstly enriched in mEC media, followed by plating onto CHROMAgar 0157 for E.coli O157:H7. Consequently, the suspected colonies were confirmed by polymerase chain reaction (PCR) assay using primers specific for O157:H7 to detect a fliCH7 gene. Only 15 of them confirmed as E. coli O157:H7 in PCR assay.Key words: Escherichia coli O157:H7, CHROMAgar 0157, fliCH7 gene, PCR