Isolation of cytotoxic substance from Kaliapsis sponge

Erna Prawita Setyowati (Faculty of Pharmacy, Universitas Gadjah Mada Sekip Utara Yogyakarta, 55281. Phone: [phone])
Umar Anggara Jenie (Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta Indonesian Institute of Science)
Sudarsono . (Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta)
Broto Kardono (Indonesian Institute of Science)
Rachmaniar Rahmat (Indonesian Institute of Science)
Edy Meiyanto (Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta)

INDONESIAN JOURNAL OF PHARMACY
Vol 18 No 4, 2007

An isolation of cytotoxic substance of Kaliapsis sponge has been conducted. The substance was isolated using maceration, partition, vacuum liquid chromatography and preparative thin layer chromatograpy methods.The result of research showed that peak 1 has the most cytotoxic activity. Cytotoxicity test with MTT [3 (4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromide] reagen on myeloma cell showed that the peak 1 had a high activity against myeloma cell. It has IC50 equal to 0,18 μg/mL.Key word: Kaliapsis sponge, cytotoxic, myeloma cell