INDONESIAN JOURNAL OF PHARMACY
Vol 18 No 3, 2007

Antioxidant–free radical scavenging of flavonoid from The Leaves of Stelechocarpus burahol (Bl.) Hook f. & Th.

Sunarni, Titik (Faculty of Pharmacy Setia Budi University Jl. Letjen Sutoyo Mojosongo – Solo, 57127, Phone. 0271-852518, HP:08121520941)
Pramono, Suwidjiyo (Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta)
Asmah, Ratna (Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta)



Article Info

Publish Date
01 Jul 2007

Abstract

The activity testing of flavonoid compounds as antioxidant and as scavenger of free radical, isolated from the Stelechocarpus burahol leaves had been performed. The aqueous extract of the leaves was concentrated and then suspended in ethanol to produce ethanolic fraction. The fraction was chromatographed on several paper chromatography systems to produce isolate with chromatographic purity.The all isolated flavonoid was identified by paper chromatography system. Especially, the B4b isolate were identified further using spectrometer UV-Vis, FT-IR and ¹H-NMR. Their antioxidant activities were done by the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method.The all isolated flavonoid showed activity as DPPH scavenger. Among these isolate, B4b exhibited a strong free radical scavenging with an EC50 value of 6.43 μg/mL. They were identified and B4b isolate was pressumed as flavon with hydroxyl group on C-3, C-7, C-3', C-4' and methyl on C-5.Key words : Stelechocarpus burahol, flavonoid, antioxidant, DPPH

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Journal Info

Abbrev

3

Publisher

Subject

Medicine & Pharmacology

Description

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas ...