ISOLATION, SELECTION AND MOLECULAR IDENTIFICATION OF ANTIBIOTIC PRODUCING ACTINOMYCETES

Tri Ratna Sulistiyani (Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jalan Raya Bogor-Jakarta Km. 46, Cibinong 16911 Indonesia)
Nunuk Widhyastuti (Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jalan Raya Bogor-Jakarta Km. 46, Cibinong 16911 Indonesia)

Widyariset
Vol 14, No 3 (2011): Widyariset

Microorganisms is one of the biodiversity that is priceless. One type of microorganisms is actinomycetes. Actinomycetes is a potential source of bioactive compounds producing high comercial value in pharmaceuticals and agricultures. The results showed that 44 Actinomycetes isolates has the ability to produce secondary metabo-lites antibiotics. They were active against M. luteus, S. aureus, E. coli, B. subtilis, S. cerevisiae, and C. albicans. Morphological studied indicated that the A16.1 isolate belonged to the genus Streptomyces. The identifi cation result based on 16S rDNA sequence is S. hygroscopicus subsp. azalomyceticus with 97% similarity level.