Buletin Kebun Raya
Vol 16, No 2 (2013): Buletin Kebun Raya Vol. 16 (2) July 2013

KERAGAMAN GENETIK AKSESI EKINASE (Echinacea purpurea (L.) Moench) HASIL SELEKSI MASSA TAHUN I MELALUI ANALISIS RAPD

Subositi, Dyah (Unknown)
Widiyastuti, Yuli (Unknown)



Article Info

Publish Date
01 Jul 2013

Abstract

Ekinase (Echinacea purpurea (L.) Moench) a medicinal plant that has immunostimulatory activity. This plant has been cultivating in Tawangmangu region by Medicinal Plant and Traditional Medicine Research and Development Office since 2002. Ten accessions of E. purpurea were found based on their morphological variation, three accessions of them are selected as promising accessions namely BH2, BHU3 dan BHU5. The objective of this research was to observe the genetic diversity of  those  promising accessions and 8 variants accession from mass selection year I using RAPD analysis. Those accessions were amplified using 10 RAPD primers. A total of 64 scorable fragments were generated from 9 RAPD primers, among which 48 fragments (75%) were polymorphic. The Dice coefficient was used to calculated the genetic similarity and UPGMA was used to generate the dendogram. The genetic similarity index among accessions evaluated ranged from 75,49-84,21% thus indicating that low level of genetic diversity. RAPD analysis proved to be efficient for genetic diversity of ekinase accessions from mass selection year I.

Copyrights © 2013






Journal Info

Abbrev

BKR

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Buletin Kebun Raya adalah publikasi ilmiah resmi dari Pusat Konservasi Tumbuhan Kebun Raya - LIPI. Jurnal ini terbit 2 kali setiap tahun, 2 nomor setiap volume, berisi tulisan ilmiah hasil penelitian, ulasan, atau gagasan asli tentang konservasi tumbuhan, biologi tumbuhan, dan pengembangan ...