Garuda - Garba Rujukan Digital

KERAGAMAN GENETIK AKSESI EKINASE (Echinacea purpurea (L.) Moench) HASIL SELEKSI MASSA TAHUN I MELALUI ANALISIS RAPD Subositi, Dyah; Widiyastuti, Yuli
Buletin Kebun Raya Vol 16, No 2 (2013): Buletin Kebun Raya Vol. 16 (2) Juli 2013
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Ekinase (Echinacea purpurea (L.) Moench) a medicinal plant that has immunostimulatory activity. This plant has been cultivating in Tawangmangu region by Medicinal Plant and Traditional Medicine Research and Development Office since 2002. Ten accessions of E. purpurea were found based on their morphological variation, three accessions of them are selected as promising accessions namely BH2, BHU3 dan BHU5. The objective of this research was to observe the genetic diversity ofÂ thoseÂ promising accessions and 8 variants accession from mass selection year I using RAPD analysis. Those accessions were amplified using 10 RAPD primers. A total of 64 scorable fragments were generated from 9 RAPD primers, among which 48 fragments (75%) were polymorphic. The Dice coefficient was used to calculated the genetic similarity and UPGMA was used to generate the dendogram. The genetic similarity index among accessions evaluated ranged from 75,49-84,21% thus indicating that low level of genetic diversity. RAPD analysis proved to be efficient for genetic diversity of ekinase accessions from mass selection year I.

KERAGAMAN GENETIK DRINGO (Acorus calamus L.) YANG DIGUNAKAN SEBAGAI TUMBUHAN OBAT PADA BEBERAPA ETNIS DI INDONESIA BERDASARKAN INTER-SIMPLE SEQUENCE REPEATS (ISSR) Subositi, Dyah; Mujahid, Rohmat; Widiyastuti, Yuli
Buletin Kebun Raya Vol 18, No 2 (2015): Buletin Kebun Raya Vol. 18 (2) July 2015
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Dringo (Acorus calamus L.) used as medicinal plant in Indonesian ethnic groups. Those information based on Ristoja 2012 research. The objective of Ristoja was to provide a database of local ethnomedicine knowledge, herbal formula and medicinal plant in Indonesia. Inter Simple Sequence Repeats (ISSR) markers were used to assess the genetic diversity of Dringo from 20 selected ethnic groups in Indonesia. Ten selected ISSR primers generated 82 amplified fragments with 51,2% were polymorphic. Dice coefficient was used to calculate similarity index and UPGMA was used to construct a dendogram. The genetic similarity index among accessions ranged from 76,7-100% thus indicating that low level of genetic diversity in dringo. Genetic diversity database can be useful for medicinal plant mapping and conservation especially for in situ conservation.

KERAGAMAN GENETIK AKSESI EKINASE (Echinacea purpurea (L.) Moench) HASIL SELEKSI MASSA TAHUN I MELALUI ANALISIS RAPD Subositi, Dyah; Widiyastuti, Yuli
Buletin Kebun Raya Vol 16, No 2 (2013): Buletin Kebun Raya Vol. 16 (2) July 2013
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Ekinase (Echinacea purpurea (L.) Moench) a medicinal plant that has immunostimulatory activity. This plant has been cultivating in Tawangmangu region by Medicinal Plant and Traditional Medicine Research and Development Office since 2002. Ten accessions of E. purpurea were found based on their morphological variation, three accessions of them are selected as promising accessions namely BH2, BHU3 dan BHU5. The objective of this research was to observe the genetic diversity ofÂ thoseÂ promising accessions and 8 variants accession from mass selection year I using RAPD analysis. Those accessions were amplified using 10 RAPD primers. A total of 64 scorable fragments were generated from 9 RAPD primers, among which 48 fragments (75%) were polymorphic. The Dice coefficient was used to calculated the genetic similarity and UPGMA was used to generate the dendogram. The genetic similarity index among accessions evaluated ranged from 75,49-84,21% thus indicating that low level of genetic diversity. RAPD analysis proved to be efficient for genetic diversity of ekinase accessions from mass selection year I.

Karakterisasi Genetik Tempuyung (Sonchus arvensis L.) Berdasarkan Penanda Molekuler Sequence-Related Amplified Polymorphism Subositi, Dyah; Mujahid, Rohmat
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Tempuyung (Sonchus arvensis L.) is known as an important medicinal plant used as a diuretics andantihypertensives. This plant is widely distributed in Indonesia. Genetic diversity of tempuyung is importantinformation as a database for further research especially in medicinal plant standardization. The objective of thisstudy was to analyse genetic characterization of tempuyung based on SRAP (Sequence-related amplifiedpolymorphism) molecular markers. Thirteen samples were collected from 8 different locations and amplified using5 primer SRAP combinations. Similarity matrix was calculated using Dice coefficient. Unweighted Pair GroupMethod Using Arithmetic Mean (UPGMA) cluster analysis was performed to develop a dendrogram. The resultindicates that there was a genetic variation among tempuyung accessions and divided into 4 clusters with similarityindex of 0,7719. Citeureup and Turen3 accessions were the most closely similar with similarity index of 0,8936. Inconclusion, SRAP markers may serve as an efficient and effective tools to analyze the genetic diversity amongtempuyung accessions.Keywords: genetic characterization, tempuyung (Sonchus arvensis L.), SRAP

KERAGAMAN GENETIK ALANG-ALANG (Imperata cylindrica (L.) Beauv.) BERDASARKAN MARKA INTER-SIMPLE SEQUENCE REPEATS (ISSR) Subositi, Dyah; Widodo, Harto
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Alang-alang (Imperata cylindrica (L.) Beauv.) has been widely used as a medicinal plant to treat some diseases, such as fever, headache, and diuretic. Nowadays, there is no information of genetic diversity of this plant used in herbal formula by ethnic groups in Indonesia. The main objective of this study was to asses genetic diversity of alang-alang from 18 selected ethnic groups in Indonesia based on Inter Simple Sequence Repeats (ISSR). Location of sample collection was identified by using data on Research on Medicinal Plant (Ristoja) 2012. Total DNA genome was isolated and ISSR primer screening were done on collected samples. Ten selected ISSR primers produced 74 amplified DNA fragments 58, fragments (78.4 %) were polymorphic. Dice index similarity was used to construct UPGMA dendrogram. The genetic similarity indexing which among accessions was ranged from 70.5â90.5% thereby indicating a low level of genetic diversity occurred in alang-alang. The results of this study also showed that ISSR markers were able to genetically differentiate alang-alang accessions by which this information can be useful for further researchs such as for standardization of medicinal plants.Â

KARAKTERISASI GENETIK TEMPUYUNG (SONCHUS ARVENSIS L.) BERDASARKAN PENANDA MOLEKULER SEQUENCE-RELATED AMPLIFIED POLYMORPHISM Subositi, Dyah; Mujahid, Rohmat
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v9i2.163

Tempuyung (Sonchus arvensis L.) is known as an important medicinal plant used as a diuretics andantihypertensives. This plant is widely distributed in Indonesia. Genetic diversity of tempuyung is importantinformation as a database for further research especially in medicinal plant standardization. The objective of thisstudy was to analyse genetic characterization of tempuyung based on SRAP (Sequence-related amplifiedpolymorphism) molecular markers. Thirteen samples were collected from 8 different locations and amplified using5 primer SRAP combinations. Similarity matrix was calculated using Dice coefficient. Unweighted Pair GroupMethod Using Arithmetic Mean (UPGMA) cluster analysis was performed to develop a dendrogram. The resultindicates that there was a genetic variation among tempuyung accessions and divided into 4 clusters with similarityindex of 0,7719. Citeureup and Turen3 accessions were the most closely similar with similarity index of 0,8936. Inconclusion, SRAP markers may serve as an efficient and effective tools to analyze the genetic diversity amongtempuyung accessions.Keywords: genetic characterization, tempuyung (Sonchus arvensis L.), SRAP

Medicinal Plants used for Antihypercholesterolemia in Ethnic Groups of Celebes Island, Indonesia: Pemanfaatan Tumbuhan Obat sebagai Antihiperkolesterolemia di Berbagai Etnis di Pulau Sulawesi, Indonesia Rahmawati, Nuning; Haryanti, Sari; Yanti Marfuatush Sholikhah, Ika; Subositi, Dyah; Widodo, Harto; Mujahid, Rohmat; Widiyastuti, Yuli
Jurnal Tumbuhan Obat Indonesia Vol. 16 No. 2 (2023): December 2023
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v16i2.1025

This study aimed to identify the utilization of medicinal plants used by selected traditional healers who met inclusion criteria to treat high cholesterol levels in ethnic groups of Celebes Island, Indonesia. Data collection was carried out through interviews, observation, and sample collection. This study revealed 67 concoction information and identified the use of 48 plant species distributed in 32 families among 39 healers in 20 ethnic groups in Celebes Island. Peperomia pellucida (L.) Kunth (UV=0.21), lamiaceae (15.63%), and leaves (57.35%) were determined as the most prominent species, family, and plant part used. The most widely prescribed rule of use is internal administration (94.03%) with a frequency of 3x1 per day (44.78%) for a week to a month (56.72%). However, conservation efforts need to be initiated immediately since more than 50% of healers made no cultivation efforts

Autentikasi Centella asiatica (L.) Urb. (Pegagan) dan Adulterannya Berdasarkan Karakter Makroskopis, Mikroskopis, dan Profil Kimia Maruzy, Anshary; Budiarti, Mery; Subositi, Dyah
Jurnal Kefarmasian Indonesia VOLUME 10, NOMOR 1, FEBRUARI 2020
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v10i1.1830

Pegagan or Centella asiatica (L.) Urb. empirically used as medicinal plant and scientifically can be used as antihypertension based on the results of the Jamu Saintification. Raw materials from C. asiatica have the potential to occur adulteration or incorrect use of plants species. There are two species of plants that have the potential to become adulterants of C. asiatica, namely Hydrocotyle verticillata and Merremia emarginata. Thus, the purpose of this study is to authenticate from Pegagan by comparing the macroscopic, microscopic, and chemical profiles of C. asiatica, H. verticillata, and M. emarginata. The method of data collection is done by purposive sampling using form intruments to record macroscopic and microscopic characters, while the chemical profiles are tested using thin layer chromatography methods to determine the content of asiaticosides in both potential adulterants species. The research was conducted at three sampling points: Central Java (Tawangmangu), West Java (Bogor), and East Java (Malang), while for laboratory research activities carried out at B2P2TOOT, Tawangmangu. The results showed macroscopically unique and easy distinguishing characters between C. asiatica and potential adulterant species H. verticillata is a leaf shape, whereas C. asiatica with M. emarginata are leaf-type appearance characters. The character of the simplicia leaf shape between C. asiatica and H. verticillata can be a distinct differentiator characters. Anatomical characters and powder fragments that can be used as a differentiator characters between C. asiatica and H. verticillata are the presence of pith on the stalk and stolon, the type of stomata, and the presence of trichomes. Authentication of simplicia powder of H. verticillata as an adulterant potential of C. asiatica with TLC, can be easily known by the absence of asiaticosides in these simplicia so that H. verticillata cannot be a substitute species for C. asiatica

ANTIMALARIAL MEDICINAL PLANTS USED BY TRADITIONAL HEALERS IN BENGKULU PROVINCE OF INDONESIA Susanti, Dian; Putra, Aditya Dwi Permana; Safrina, Devi; Wijaya, Nur Rahmawati; Adi, M Bakti Samsu; Mujahid, Rohmat; Rukmana, Rizal Maarif; Subositi, Dyah; Haryanti, Sari; Siswanto, Usman; Widiyastuti, Yuli
BIOTROPIA Vol. 31 No. 3 (2024): BIOTROPIA Vol. 31 No. 3 December 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.3.2318

Article Highlights- This research provides information on the use of medicinal plants for treating malaria among ethnic groups in Bengkulu Province.- This research was part of RISTOJA (Research on Medicinal Plants), national research conducted by the Ministry of Health of the Republic of Indonesia.- Several compounds found in medicinal plants used by traditional healers in the Bengkulu Province have been scientifically proven to be effective as antimalarial medication.AbstractMalaria is a disease with a high mortality rate and is still a significant problem in Indonesia and globally. One of the provinces in Indonesia that uses a diversity of medicinal plants to treat malaria is Bengkulu Province. This research aims to inventory and evaluate the use of medicinal plants as malaria drugs in Bengkulu Province. The research methods used were observation, interviews, and the collection of samples. Respondents were traditional healers from seven ethnic groups in Bengkulu. Plant species, use value (UV) parameters, fidelity level (FL), family use value (FUV), relative frequency of citation (RFC), and plant parts value (PPV) were analyzed. This study found 32 specific herbal concoctions and identified the use of 47 plant species in 28 families used by 20 healers in 6 ethnic groups of Bengkulu. The species Carica papaya, Peronema canescens, and Tinospora crispa have the highest use value, fidelity level, and relative frequency of citation. The Lamiaceae and Caricaceae families have the highest family use value (0.15). The leaves (43.9%) and barks (13.6%) were the most frequently utilized parts of medicinal plants to overcome malaria. Decoction was the method most widely used by traditional healers in Bengkulu to prepare conventional medicine formula.

Respon Pertumbuhan, Produksi dan Kualitas Daun Duduk (Desmodium triquetrum (L.) D.C.) terhadap Ketinggian Tempat Budidaya Fauzi; Subositi, Dyah
Jurnal Jamu Indonesia Vol. 4 No. 2 (2019): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v4i2.129

Daun duduk (Desmodium triquetrum (L.) DC.) merupakan tumbuhan obat yang mengandung senyawa flavonoid, saponin, polifenol dan trigonelin. Tanaman ini di Indonesia tersebar di pulau Jawa, Madura dan Sumatera. Tumbuh dari daerah dataran rendah sampai dataran tinggi pada ketinggian 5 - 1.500 m dpl, sampai saat ini belum banyak dibudidayakan. Penelitian ini bertujuan untuk mengetahui ketinggian tempat tumbuh yang optimal bagi pertumbuhan, produksi dan kualitas daun duduk. Penelitian dilaksanakan di kebun percobaan Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional pada bulan Januari – September 2016. Percobaan menggunakan Rancangan Acak Kelompok, faktor perlakuan yang dicobakan adalah ketinggian lokasi penanaman yaitu 200, 450 dan 1.200 m dpl. Hasil penelitian menunjukkan bahwa ketinggian tempat berpengaruh nyata terhadap pertumbuhan, produksi dan kadar flavonoid daun duduk. Tinggi tanaman tertinggi (179,90 cm) diperoleh pada penanaman di ketinggian 1.200 m dpl. Jumlah dan bobot kering daun tertinggi dihasilkan pada penanaman di ketinggian 200 m dpl. Kadar sari larut air (19,73 %), kadar sari larut etanol (18,28 %), dan kadar flavonoid (0,249 %) yang tertinggi diperoleh di ketinggian 450 m dpl.

Title

Found 15 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 15 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 15 Documents
Search