AGRIVITA, Journal of Agricultural Science
Vol 45, No 3 (2023)

Diversity of Culturable Bacterial Gut Microbiome of Oryctes rhinoceros Larvae and Their Potency as Source of Protease and Lipase Enzymes

Luqman Qurata Aini (Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang, East Java, Indonesia (Scopus ID: 35071053600))
Fibrianti Shinta Dewi (Plant Pathology Study Program, Faculty of Agriculture, Universitas Brawijaya, Malang, East Java, Indonesia)
Irisa Trianti (Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang, East Java, Indonesia)
Ito Fernando (Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang, East Java, Indonesia)
Yogo Setiawan (Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang, East Java, Indonesia)



Article Info

Publish Date
31 Oct 2023

Abstract

Oryctes rhinoceros is an important pest of various coconut and palm species. Studies on the bacterial gut microbiome of O. rhinoceros larvae and their potential use as producers of industrial enzymes are still limited. This study aims to examine the diversity and abundance of culturable bacterial microbiome in the gut of O. rhinoceros and their potential to produce protease and lipase. This study consisted of several stages, i.e., collection of O. rhinoceros larvae from coconut plantations; isolation of culturable bacteria from larval gut; protease and lipase enzyme activity assays; and partial characterization of culturable gut bacteria in terms of morphology, physiology, and biochemistry. There were seven bacteria that were isolated from the gut of O. rhinoceros, which were differentiated by their morphotype. The seven bacterial isolates also showed variations in physiological, and biochemical characteristics, and the larval guts were dominated by Gram-positive bacteria. The Shannon diversity index was 1.33, evenness was 0.682, richness was 7, and the abundance of each type of bacteria varied between 2x105 and 9.1x1010. Bacteria UBOG1, UBOG3, and UBOG5 were able to produce protease, but all seven bacteria were unable to produce lipase. Thus, bacterial isolates UBOG1, UBOG3, and UBOG5 are potential to be used in industry.

Copyrights © 2023






Journal Info

Abbrev

AGRIVITA

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research ...