BS endophytic Fungi is a Fungi isolated from the flower of the bitter plant (A. Paniculata) which is known to produce various secondary metabolites which are reported to have antibacterial bioactivity. This study aimed to determine the antibacterial activity of extract from endophytic Fungi BS isolated from bitter flower (A. Paniculata) on brown rice growing media against Streptococcus mutans, Staphylococcus aureus, and Pseudomonas aeruginosa bacteria. The method used is endophytic Fungi BS grown on media extracted by maceration method with solvent. The antibacterial activity test of the extract was carried out by disc diffusion method. The results obtained showed that the extract of the endophytic Fungi BS grown on brown rice media gave activity to inhibit the growth of Streptococcus mutans, Staphylococcus aureus, and Pseudomonas aeruginosa bacteria at concentrations of 10%, 30%, and 50%. In conclusion, the extract of BS endophytic Fungi isolated from A. paniculata flowers has potential as an antibacterial
                        
                        
                        
                        
                            
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